Diverse strategies adopted by nature for regulating purine biosynthesis via fine-tuning of purine metabolic enzymes

Singh, Sawraj; Anand, Ruchi

doi:10.1016/j.cbpa.2022.102261

Cited by 5 publications

(2 citation statements)

References 53 publications

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“…In addition, the compounds identi ed by KEGG lacked adenosine and adenine. Adenine is a nitrogenous base found in microbial RNA, while adenosine is a nucleoside composed of adenine and ribose sugar, both precursors to the cellular energy molecule adenosine triphosphate (ATP) 41 . This phenomenon could be attributed to NH 4 + , compared with NO 3 − , more effectively stimulating the synthesis metabolism of adenine and adenosine to generate energy, which can be used by MOB and other microbes.…”

Section: In Methane Oxidation Induced By Nitrogen Formsmentioning

confidence: 99%

Enhanced Aerobic Methane Oxidation: Influence of Ammonium Introduction and Complete Nitrification Coupling on Metabolic Activity

Xu,

Bai,

Huang

et al. 2024

Preprint

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The biological process of methane (CH4) oxidation is crucial for reducing fugitive CH4 emissions into the atmosphere. Within the nitrogen cycle, ammonium (NH4+) and nitrate (NO3-) significantly influence the growth and activity of methane-oxidizing bacteria (MOB). However, the stimulatory effects of NH4+ and NO3- on aerobic CH4 oxidation and the associated metabolic remain to be comprehensively elucidated. In this study, NH4+ and NO3- were loaded onto zeolites and mixed with MOB-rich soil to conduct CH4 incubation experiments. The CH4 oxidation rate and metabolomic changes during the second cycle, characterized by distinct MOB activities, were determined. The NH4+-added group showed a significant increase in the CH4 oxidation rate (up to 1.25 mg CH4·g-1·d-1) compared with the NO3--added group. Analysis of the microbial community and metabolomics revealed that NH4+ stimulated the population of two electroactive bacterial Clostridia and Thermincolales within the Firmicutes phylum. On the metabolic level, NH4+ was noted to promote nucleotide and active amino acid metabolic pathways. Nitrospirota drove the synthesis of glucogenic and ketogenic amino acids, which could be used by the MOB in the tricarboxylic acid cycle. Additionally, this study clarified the plausible interplay between nitrification and the CH4 oxidation process. These insights could help enhance our understanding of the interaction between the global nitrogen cycle and aerobic CH4 oxidation.

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Section: In Methane Oxidation Induced By Nitrogen Formsmentioning

confidence: 99%

Enhanced Aerobic Methane Oxidation: Influence of Ammonium Introduction and Complete Nitrification Coupling on Metabolic Activity

Xu,

Bai,

Huang

et al. 2024

Preprint

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“…Proper cellular function requires the preservation of nucleotide pool integrity by maintaining a proper supply of purine and pyrimidine nucleotides while restricting noncanonical and damaged nucleotides (Evans & Guy, 2004; Kamiya, 2003; Pavlov et al, 2016; Simone, Pavlov, & Borgstahl, 2013; Singh & Anand, 2023). There are a number of enzymes that function in nucleotide pool maintenance and one such enzyme is inosine triphosphate pyrophosphatase (ITPase; reviewed in Burgis, 2016; Sakumi et al, 2010; Simone, Pavlov, & Borgstahl, 2013; Pavlov et al, 2016; Zamzami, 2022).…”

Section: Introductionmentioning

confidence: 99%

Inosine triphosphate pyrophosphatase: A guardian of the cellular nucleotide pool and potential mediator of RNA function

2023

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Inosine triphosphate pyrophosphatase (ITPase), encoded by the ITPA gene in humans, is an important enzyme that preserves the integrity of cellular nucleotide pools by hydrolyzing the noncanonical purine nucleotides (deoxy)inosine and (deoxy)xanthosine triphosphate into monophosphates and pyrophosphate. Variants in the ITPA gene can cause partial or complete ITPase deficiency. Partial ITPase deficiency is benign but clinically relevant as it is linked to altered drug responses. Complete ITPase deficiency causes a severe multisystem disorder characterized by seizures and encephalopathy that is frequently associated with fatal infantile dilated cardiomyopathy. In the absence of ITPase activity, its substrate noncanonical nucleotides have the potential to accumulate and become aberrantly incorporated into DNA and RNA. Hence, the pathophysiology of ITPase deficiency could arise from metabolic imbalance, altered DNA or RNA regulation, or from a combination of these factors. Here, we review the known functions of ITPase and highlight recent work aimed at determining the molecular basis for ITPA‐associated pathogenesis which provides evidence for RNA dysfunction.This article is categorized under: RNA in Disease and Development > RNA in Disease RNA in Disease and Development > RNA in Development

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Metabolomics study of dietary Pleurotus eryngii β-type glycosidic polysaccharide on colitis induced by dextran sodium sulfate in mice – Exploration for the potential metabolic indicators in urine and serum

Ma,

Tao,

et al. 2024

Food Chemistry

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Diverse strategies adopted by nature for regulating purine biosynthesis via fine-tuning of purine metabolic enzymes

Cited by 5 publications

References 53 publications

Enhanced Aerobic Methane Oxidation: Influence of Ammonium Introduction and Complete Nitrification Coupling on Metabolic Activity

Enhanced Aerobic Methane Oxidation: Influence of Ammonium Introduction and Complete Nitrification Coupling on Metabolic Activity

Inosine triphosphate pyrophosphatase: A guardian of the cellular nucleotide pool and potential mediator of RNA function

Metabolomics study of dietary Pleurotus eryngii β-type glycosidic polysaccharide on colitis induced by dextran sodium sulfate in mice – Exploration for the potential metabolic indicators in urine and serum

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