Diverse Soybean Accessions Identified with Temperature‐Sensitive Resistance to <i>Tobacco Streak Virus</i>

Hobbs, H. A.; Jossey, S.; Wang, Y.; Hartman, G. L.; Domier, Leslie L.

doi:10.2135/cropsci2011.05.0265

Cited by 8 publications

(2 citation statements)

References 30 publications

(41 reference statements)

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“…The nRT-PCR and WI qPCR assay can be used to monitor TSV infection in soybean fields and rogue infected plants to avoid yield loss and maintain seed production fields free from the virus. Another strategy to control viruses is to use resistant cultivars (Rubio et al 2020), however information about resistance to TSV is scarce in soybean germplasm/cultivars (Hobbs et al 2012;Rabedeaux et al 2005;Wang et al 2005). Viral load quantification can provide information about resistance levels in germplasm and cultivars (Rubio et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Sensitive and Specific qPCR and Nested RT-PCR Assays for the Detection of Tobacco Streak Virus in Soybean

et al. 2021

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Tobacco streak virus (TSV) is a re-emerging and understudied pathogen of soybean (Glycine max). Management of TSV is challenging due to the multiple modes of transmission, widespread susceptibility of commercial soybean, and lack of reliable diagnostic tests for the virus. Soybean plants with TSV-like, virus-like, or no symptoms were collected from commercial and research fields in seven counties in Wisconsin. Two sensitive assays were developed for the detection of TSV: a fluorescent dye-based quantitative RT-PCR (qPCR) assay and a nested RT-PCR (nRT-PCR). Tobacco streak virus was detected in 47% and 91% of symptomatic samples using the qPCR assay and the nRT-PCR assay, respectively, suggesting that the nRT-PCR assay has higher sensitivity for detecting TSV. The qPCR assay’s limit of detection was determined at 10 fg and the assay was used to estimate the viral load in TSV-symptomatic samples. The titer of TSV in these samples was determined by absolute quantification and ranged from 15 fg to 0.796 ng. The two assays reported here provide diagnostic tools for the rapid and accurate detection of TSV that can aid in monitoring outbreaks, assessing management strategies, or screening soybean cultivars/accessions for resistance to the virus.

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Section: Discussionmentioning

confidence: 99%

Sensitive and Specific qPCR and Nested RT-PCR Assays for the Detection of Tobacco Streak Virus in Soybean

et al. 2021

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“…Naturally occurring genetic resistance to TSV has been reported from the USA in soybean germplasm (Hobbs et al 2012), and from India in wild Arachis (peanut) germplasm (Kalyani et al 2007; Kalyani et al 2005) and sunflower germplasm (Karuna et al 2008;. The sunflower resistance screening by was done in a single trial with relatively low disease pressure and non-replicated plots.…”

Section: Use Of Genetic Resistancementioning

confidence: 99%

Epidemiology and genetic diversity of Tobacco streak virus and related subgroup 1 ilarviruses

Sharman¹

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A quarter of Australia's sunflower production is from the central highlands region of Queensland and is currently worth six million dollars ($AUD) annually. From the early 2000s a severe necrosis disorder of unknown aetiology was affecting large areas of sunflower crops in central Queensland, leading to annual losses of up to 20%. Other crops such as mung bean and cotton were also affected. This PhD study was undertaken to determine if the causal agent of the necrosis disorder was of viral origin and, if so, to characterise its genetic diversity, biology and disease cycle, and to develop effective control strategies.The research described in this thesis identified Tobacco streak virus (TSV; genus Ilarvirus, family Bromoviridae) as the causal agent of the previously unidentified necrosis disorder of sunflower in central Queensland. TSV was also the cause of commonly found diseases in a range of other crops in the same region including cotton, chickpea and mung bean. This was the first report from Australia of natural field infections of TSV from these four crops.TSV strains have previously been reported from other regions of Australia in several hosts based on serological and host range studies. In order to determine the relatedness of previously reported TSV strains with TSV from central Queensland, we characterised the genetic diversity of the known TSV strains from Australia. We identified two genetically distinct TSV strains from central Queensland and named them based on their major alternative hosts, TSV-parthenium from Parthenium hysterophorus and TSV-crownbeard from Verbesina encelioides. They share only 81 % totalgenome nucleotide sequence identity. In addition to TSV-parthenium and TSV-crownbeard from central Queensland, we also described the complete genomes of two other ilarvirus species. This proved that previously reported TSV strains, TSV-S isolated from strawberry and TSV-Ag from Ageratum houstonianum, were actually the first record of Strawberry necrotic shock virus from Australia, and a new subgroup 1 ilarvirus, Ageratum latent virus. Our results confirmed that the TSV strains found in central Queensland were not related to previously described strains from Australia and may represent new incursions. This is the first report of the genetic diversity within subgroup 1 ilarviruses from Australia.Based on field observations we hypothesised that parthenium and crownbeard were acting as symptomless hosts of TSV-parthenium and TSV-crownbeard, respectively. We developed strainspecific multiplex PCRs for the three RNA segments to accurately characterise the range of naturally infected hosts across central Queensland. Results described in this thesis show compelling evidence that parthenium and crownbeard are the major (symptomless) alternative hosts of TSV-4 parthenium and TSV-crownbeard. While both TSV strains had wide natural host ranges, the geographical distribution of each strain was closely associated with the respective distribution of their major alternative hosts. Both TSV strains were ...

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Preliminary studies on resistance phenotypes to Turnip mosaic virus in Brassica napus and B. carinata from different continents and effects of temperature on their expression

2014

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Diverse Soybean Accessions Identified with Temperature‐Sensitive Resistance to Tobacco Streak Virus

Cited by 8 publications

References 30 publications

Sensitive and Specific qPCR and Nested RT-PCR Assays for the Detection of Tobacco Streak Virus in Soybean

Sensitive and Specific qPCR and Nested RT-PCR Assays for the Detection of Tobacco Streak Virus in Soybean

Epidemiology and genetic diversity of Tobacco streak virus and related subgroup 1 ilarviruses

Preliminary studies on resistance phenotypes to Turnip mosaic virus in Brassica napus and B. carinata from different continents and effects of temperature on their expression

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