Diverse responses of pqqC‐ and phoD‐harbouring bacterial communities to variation in soil properties of Moso bamboo forests

Abstract

Phosphate-mobilizing bacteria (PMB) play a critical role in the regulation of phosphorus availability in the soil. The microbial genes pqqC and phoD encode pyrroloquinoline quinone synthase and bacterial alkaline phosphatase, respectively, which regulate inorganic and organic phosphorus mobilization, and are therefore used as PMB markers. We examined the effects of soil properties in three Moso bamboo forest sites on the PMB communities that were profiled using high-throughput sequencing. We observed differentiated responses of pqqCand phoD-harbouring PMB communities to various soil conditions. There was significant variation among the sites in the diversity and structure of the phoDharbouring community, which correlated with variation in phosphorus levels and non-capillary porosity; soil organic carbon and soil water content also affected the structure of the phoD-harbouring community. However, no significant difference in the diversity of pqqC-harbouring community was observed among different sites, while the structure of the pqqC-harbouring bacteria community was affected by soil organic carbon and soil total nitrogen, but not soil phosphorus levels. Overall, changes in soil conditions affected the phoDharbouring community more than the pqqCharbouring community. These findings provide a new insight to explore the effects of soil conditions on microbial communities that solubilize inorganic phosphate and mineralize organic phosphate.

Characterization of an extremophile bacterial acid phosphatase derived from metagenomics analysis

“…At 30°C, the protein displayed maximal activity between pH 4.5 and 7.5, retaining approximately 80% activity at pH 3.5 and 40% at pH 8.5 (Figure 1). These findings suggest that FS6 exhibits unusual acid phosphatase characteristics, differing from the bell-shaped pH rate profile typically observed in acid phosphatases according to Van Etten (1982) and other researchers (Shi et al, 2022;Du Plessis et al, 2002;Reilly et al, 2009). This distinct behaviour classifies FS6 as a unique class C acid phosphatase.…”

Diversity and functional traits of indigenous soil microbial flora associated with salinity and heavy metal concentrations in agricultural fields within the Indus Basin region, Pakistan

“…It is unclear why soil D also was enriched in nirK , though it is interesting to note that this soil also had greater abundance of microbes with the gene for pyrroloquinoline-quinone synthase ( pqqC ), and the highest levels of SOM ( Table 1 ). The pqqC gene plays a critical role in SOM cycling, and not surprisingly, is often linked with greater SOC and total N levels ( Shi et al, 2022 ).…”

Phosphate-Solubilizing Bacteria: Advances in Their Physiology, Molecular Mechanisms and Microbial Community Effects

“…Yellow sandalwood rhizosphere soil ACP, ALP [98] Enterobacter sp. rhizosphere soil of moso bamboo ACP [99] 3.1.…”

“…Moreover, pqqA and pqqC are the two key genes; the gene pqqA consists of 22 amino acids, the peptides of glutamic and tyrosine that provide carbon and nitrogen to the PQQ biological system, and the gene pqqC that encodes the pyrroloquinoline synthase C (PQQC) gene, which catalyzes the conversion of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9dicarboxylic acid to pyrroloquinoline quinone (PQQ) [109]. It has been shown that the pqqC gene is highest in many PSB, such as Burkholderia (8.98-16.69%), Azotobacter (5.27-11.05%), Pseudomonas aeruginosa (3.31-9.28%) and Mycobacterium (1.25-9.8%), and the pqqC gene has also been identified to track the inorganic phosphate-solubilizing ability marker genes of microorganisms [99]. Joshi et al found 16 bacterial isolates with 82 bp pqqC gene amplification positivity and 6 bacterial isolates with 72 bp pqqA gene amplification positivity.…”