Diverse Physiological Functions of Cation Proton Antiporters across Bacteria and Plant Cells

Tsujii, Masaru; Tanudjaja, Ellen; Uozumi, Nobuyuki

doi:10.3390/ijms21124566

Cited by 25 publications

(12 citation statements)

References 99 publications

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“…In contrast to halophytes which tolerate higher chloroplastic Na + concentrations, photosynthesis in glycophytes (including many C 3 crop plants) becomes impaired by subtle elevation of stromal Na + from 0.21 to 0.38mM in Arabidopsis ( Mueller et al, 2014 ). The NHD1 Na + /H + antiporter on the chloroplast envelope is active in Na + extrusion ( Figure 2 ), maintaining a positive Na + gradient for other Na + -dependent carriers on the chloroplast envelope, regulating stromal pH, and contributing to salt tolerance ( Höhner et al, 2016 ; Tsujii et al, 2020 ). This suggests that, in particular, light/dark regulated Na + extrusion and Na + /HCO 3 − symport need to be synchronized.…”

Section: What Are the Energetic And Functional Requirements Of C I Uptake Systems?mentioning

confidence: 99%

Engineered Accumulation of Bicarbonate in Plant Chloroplasts: Known Knowns and Known Unknowns

et al. 2021

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Heterologous synthesis of a biophysical CO2-concentrating mechanism (CCM) in plant chloroplasts offers significant potential to improve the photosynthetic efficiency of C3 plants and could translate into substantial increases in crop yield. In organisms utilizing a biophysical CCM, this mechanism efficiently surrounds a high turnover rate Rubisco with elevated CO2 concentrations to maximize carboxylation rates. A critical feature of both native biophysical CCMs and one engineered into a C3 plant chloroplast is functional bicarbonate (HCO3−) transporters and vectorial CO2-to-HCO3− converters. Engineering strategies aim to locate these transporters and conversion systems to the C3 chloroplast, enabling elevation of HCO3− concentrations within the chloroplast stroma. Several CCM components have been identified in proteobacteria, cyanobacteria, and microalgae as likely candidates for this approach, yet their successful functional expression in C3 plant chloroplasts remains elusive. Here, we discuss the challenges in expressing and regulating functional HCO3− transporter, and CO2-to-HCO3− converter candidates in chloroplast membranes as an essential step in engineering a biophysical CCM within plant chloroplasts. We highlight the broad technical and physiological concerns which must be considered in proposed engineering strategies, and present our current status of both knowledge and knowledge-gaps which will affect successful engineering outcomes.

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Section: What Are the Energetic And Functional Requirements Of C I Uptake Systems?mentioning

confidence: 99%

Engineered Accumulation of Bicarbonate in Plant Chloroplasts: Known Knowns and Known Unknowns

et al. 2021

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“…In addition, all halophilic microorganisms contain powerful transport mechanisms, usually based on Na + /H + antiports, to expel sodium ions from inside the cell [62]. Majority of functions responsible for the survival of halophilic and halotolerant bacteria in their environment are encoded by megaplasmids [63].…”

Section: Microbiota Tolerance Strategies Associated With Halophytesmentioning

confidence: 99%

Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application

et al. 2021

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Plant growth promoting bacteria (PGPB) have been the target of intensive research studies toward their efficient use in the field as biofertilizers, biocontrol, and bioremediation agents among numerous other applications. Recent trends in the field of PGPB research led to the development of versatile multifaceted PGPB that can be used in different field conditions such as biocontrol of plant pathogens in metal contaminated soils. Unfortunately, all these research efforts lead to the development of PGPB that failed to perform in salty environments. Therefore, it is urgently needed to address this drawback of these PGPB toward their efficient performance in salinity context. In this paper we provide a review of state-of-the-art research in the field of PGPB and propose a road map for the development of next generation versatile and multifaceted PGPB that can perform in salinity. Beyond soil desalinization, our study paves the way towards the development of PGPB able to provide services in diverse salty environments such as heavy metal contaminated, or pathogen threatened. Smart development of salinity adapted next generation biofertilizers will inevitably allow for mitigation and alleviation of biotic and abiotic threats to plant productivity in salty environments.

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“…Many cation/proton antiporters have been identified in bacteria (Krulwich et al 2009;Tsujii et al 2020). Cation/ proton antiporters contribute to maintaining the appropriate intracellular pH homeostasis particularly in alkaline environments that require import of hydrogen ions from a comparatively proton-poor external environment.…”

Section: Co-transport Mechanismsmentioning

confidence: 99%

Genomic analysis of a functional haloacid-degrading gene of Bacillus megaterium strain BHS1 isolated from Blue Lake (Mavi Gölü, Turkey)

Wahhab

Samsulrizal

Edbeib³

et al. 2021

Ann Microbiol

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Purpose Bacillus megaterium strain BHS1, isolated from an alkaline water sample taken from Mavi Gölü (Blue Lake, Turkey), can grow on minimal medium containing 2,2-dichloropropionic acid. We characterized this bacterium at the genomic level. Methods The HiSeq platform was used to carry out genome sequencing, de novo assembly, and scaffolding with strain BHS1. Next, genome data were analyzed to demarcate DNA regions containing protein-coding genes and determine the function of certain BHS1 genes. Finally, results from a colorimetric chloride ion–release assay demonstrated that strain BHS1 produces dehalogenase. Results De novo assembly of the BHS1 genomic sequence revealed a genome size of ~ 5.37 Mb with an average G+C content of 38%. The predicted nuclear genome harbors 5509 protein-coding genes, 1353 tRNA genes, 67 rRNA genes, and 6 non-coding (mRNA) genes. Genomic mapping of strain BHS1 revealed its amenability to synthesize two families of dehalogenases (Cof-type haloacid dehalogenase IIB family hydrolase and haloacid dehalogenase type II), suggesting that these enzymes can participate in the catabolism of halogenated organic acids. The mapping identified seven Na+/H+ antiporter subunits that are vital for adaptation of the bacterium to an alkaline environment. Apart from a pairwise analysis to the well-established L-2-haloacid dehalogenases, whole-cell analysis strongly suggested that the haloacid dehalogenase type II might act stereospecifically on L-2-chloropropionic acid, D,L-2-chloropropionic acid, and 2,2-dichloropropionic acid. Whole-cell studies confirmed the utilization of these three substrates and the gene’s role in dehalogenation. Conclusions To our knowledge, this is the first report of the full genome sequence for strain BHS1, which enabled the characterization of selected genes having specific metabolic activities and their roles in the biodegradation of halogenated compounds.

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Diverse Physiological Functions of Cation Proton Antiporters across Bacteria and Plant Cells

Cited by 25 publications

References 99 publications

Engineered Accumulation of Bicarbonate in Plant Chloroplasts: Known Knowns and Known Unknowns

Engineered Accumulation of Bicarbonate in Plant Chloroplasts: Known Knowns and Known Unknowns

Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application

Genomic analysis of a functional haloacid-degrading gene of Bacillus megaterium strain BHS1 isolated from Blue Lake (Mavi Gölü, Turkey)

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