Diverse nitrogen signals activate convergent ROP2-TOR signaling in Arabidopsis

Liu, Yanlin; Duan, Xingwu; Zhao, Xiaolei; Ding, Wenlong; Wang, Yaowei; Xiong, Yan

doi:10.1016/j.devcel.2021.03.022

Cited by 91 publications

(120 citation statements)

References 66 publications

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“…Different from these observations in spl7-1, TOR activity is decreased under nitrogen starvation (Liu et al, 2021). While TOR is involved in the phosphorylation of a variety of targets in several downstream signaling pathways, phosphorylation of S6K is responsive to sucrose and glucose (Xiong and Sheen, 2012;Dobrenel et al, 2016) and thus a suitable marker for TOR activity in our conditions.…”

Section: Figures To 4)mentioning

confidence: 63%

Energy status-promoted growth and development of Arabidopsis require copper deficiency response transcriptional regulator SPL7

Schulten

Pietzenuk

Quintana

et al. 2021

Preprint

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Copper (Cu) is a cofactor of around 300 Arabidopsis proteins including photosynthetic and mitochondrial electron transfer chain enzymes critical for ATP production and carbon fixation. Plant acclimation to Cu deficiency requires the transcription factor SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 (SPL7). We report that in the wild type and in the spl7-1 mutant, respiratory electron flux via Cu-dependent cytochrome c oxidase remained unaffected under both normal and low-Cu cultivation conditions. Contrary to the wild type, supplementing Cu-deficient media with exogenous sugar failed to stimulate growth of spl7-1. The spl7-1 mutant accumulated carbohydrates including the signaling sugar trehalose 6-phosphate, as well as ATP and NADH, also under normal Cu supply and without sugar supplementation. Late flowering of spl7 1 was in agreement with its attenuated sugar responsiveness. Functional TOR and SnRK1 kinase signaling in spl7-1 suggested against fundamental defects in these energy-signaling hubs. Sequencing of chromatin immunoprecipitates combined with transcriptomics identified direct targets of SPL7-mediated positive regulation, including FE SUPEROXIDE DISMUTASE1 (FSD1), COPPER-DEFICIENCY-INDUCED TRANSCRIPTION FACTOR1 (CITF1) and uncharacterized bHLH23 (CITF2), as well as an enriched upstream GTACTRC motif. In summary, transducing energy availability into growth and reproductive development requires the function of SPL7. Our results could help to increase crop yields, especially on Cu-deficient soils.

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Section: Figures To 4)mentioning

confidence: 63%

Energy status-promoted growth and development of Arabidopsis require copper deficiency response transcriptional regulator SPL7

Schulten

Pietzenuk

Quintana

et al. 2021

Preprint

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“… Note: Rapamycin and several secondary generation mTOR specific inhibitors (e.g. AZD-8055, Torin1 and Torin2), have been successfully used for plant TOR signaling studies ( Fu et al., 2021 ; Li et al., 2017 ; Liu et al., 2021 ; Montané and Menand, 2019 ; Xiong et al., 2013 ). The optimized treatment time and concentration for a specific inhibitor could be experimentally verified based on the experimental design and purposes (See an example in Extended Data Figure 2 C, ( Fu et al., 2021 )).…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… (G) Seedlings grown in inorganic nitrogen-containing medium (+ N) for 4–12 DAG maintained relatively high and stable TOR kinase activity. 9 DAG of 35S::S6K1-HA seedlings were treated with 5 μM Torin2 for 1 h. Figure reprinted with permission from Liu et al. (2021) .…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… Note: The liquid medium culture systems described in this protocol can generate stable mitotic quiescent seedlings to study the underlying mechanisms of nutrient-TOR signaling in the regulation of cell proliferation in the shoot and root apexes, and have been used to study the role of glucose-TOR signaling in root meristem cell proliferation ( Fu et al., 2021 ; Xiong et al., 2013 ), and the role of nitrogen-TOR signaling in shoot apex cell proliferation ( Liu et al., 2021 ). This protocol has broad utility and can also be applied, with some modifications, to study other molecular functions of plant nutrient signaling including cell elongation ( Yuan et al., 2020 ), cell division interaction with environmental signals ( Li et al., 2017 ), and metabolite mediated circadian regulation ( Zhang et al., 2019 ).…”

Section: Before You Beginmentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Xiong et al. (2013) and Liu et al. (2021) .…”

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Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings

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Comprehensive insight into an amino acid metabolic network in postharvest horticultural products: a review

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Amino acid (AA) metabolism plays a vital role in the central metabolism of plants. In addition to protein biosynthesis, AAs are involved in secondary metabolite biosynthesis, signal transduction, stress response, defense against pathogens, flavor formation, and so on. Besides these functions, AAs can be degraded into precursors or intermediates of the tricarboxylic acid cycle to substitute respiratory substrates and restore energy homeostasis, as well as directly acting as signal molecules or be involved in the regulation of plant signals to delay senescence of postharvest horticultural products (PHPs). AA metabolism and its role in plants growth have been clarified; however, only a few studies about their roles exist concerning the postharvest preservation of fruit and vegetables. This study reviews the potential functions of various AAs by comparing the difference in AA metabolism at the postharvest stage and then discusses the crosstalk of AA metabolism and energy metabolism, the target of rapamycin/sucrose nonfermenting‐related kinase 1 signaling and secondary metabolism. Finally, the roles and effect mechanism of several exogenous AAs in the preservation of PHPs are highlighted. This review provides a comprehensive insight into the AA metabolism network in PHPs. © 2023 Society of Chemical Industry.

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Diverse nitrogen signals activate convergent ROP2-TOR signaling in Arabidopsis

Cited by 91 publications

References 66 publications

Energy status-promoted growth and development of Arabidopsis require copper deficiency response transcriptional regulator SPL7

Energy status-promoted growth and development of Arabidopsis require copper deficiency response transcriptional regulator SPL7

Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings

Comprehensive insight into an amino acid metabolic network in postharvest horticultural products: a review

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