Diverse Molecular Genotypes of<i>Mycobacterium tuberculosis</i>Complex Isolates Circulating in the Free State, South Africa

Dijk, A. Van der Spoel van; Makhoahle, Pakiso Moses; Rigouts, Leen; Baba, Kamaldeen

doi:10.1155/2016/6572165

Cited by 8 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study revealed high diversity of strains, predominantly the non-Beijing lineages: LAM (LAM3, LAM9 and LAM11-ZWE), T (T1, T2, T3 and T2/X1), CAS (CAS1-Delhi and CAS1-KILI), MANU2, U, X (X1, X2, and X3), S including the Beijing and Beijing-like families. The most common lineages observed in this study were the LAM and the T, consistent with findings of studies in the KwaZulu Natal and the Eastern Cape [ 15 – 17 ]. The results provide an overview of the genetic profile of M. tuberculosis families across the districts suggesting similar genetic profile of M. tuberculosis families in countries bordering the Limpopo Province because of ongoing migration within the province and the neighbouring countries.…”

Section: Discussionsupporting

confidence: 91%

“…Multiple mycobacterial lineages have been reported in several provinces of South Africa comprising of the Beijing and non-Beijing genotypes [ 8 , 14 – 16 ]. However, the studies on South African genetic diversity of M. tuberculosis have been pitched at provincial levels, and most researchers have not paid any attention to such diversity within the provinces except for the study in three areas of the Free State Province [ 17 ]. Furthermore, Limpopo Province of South Africa shares its borders with Zimbabwe to the north, Mozambique to the east and Botswana to the west.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity and distribution of Mycobacterium tuberculosis genotypes in Limpopo, South Africa

et al. 2017

View full text Add to dashboard Cite

BackgroundTuberculosis remains a major health problem and knowledge of the diversity of Mycobacterium tuberculosis strains in specific geographical regions can contribute to the control of the disease. This study describes the genetic profile of M. tuberculosis in five districts of Limpopo Province.MethodsA total 487 isolates were collected from the National Health Laboratory Services from all regions/districts of Limpopo Province. Only 215 isolates were confirmed to be M. tuberculosis by Bactec Mycobacterium Growth Indicator Tube 960® and Rhodamine-Auramine staining. Isolates were subcultured on Löwenstein-Jensen medium agar slants to validate purity. They were spoligotyped and data analysed using the international spoligotyping database 4 (SpolDB4).ResultsOf the 215 isolates, 134 (62.3%) were genotyped into 21 genotype families while 81 (37.7%) were orphans. The 81 orphans were further subjected to resolution employing SpolDB3/RIM. Overall, the study revealed a high diversity of strains of 32 predominantly the non-Beijing lineages: the LAM- LAM3 (9.8%), LAM9 (4.7%) and LAM11- ZWE (3.3%), the T-T1(15.0%), T2 (0.9%), T2-T3 (1.4%), the CAS-CAS1-Delhi 5 (1.9%) and CAS1-KILI (1.4%) the MANU2 (1.4%), U (0.5%), X-X1(1.4%), X3 (1.9%), S (9.8%), CAS (1.4%), LAM7(0.9%), T3(0.5%), LAM8(4.7%), T4(1.4%), X2(0.4%), AI5(1.9%), LAM1(0.5%), FAMILY33 (1.9%), EAI4(1.4%), M. microti (1.9%). The Beijing and Beijing-like families were (14.9%) and (0.9%), respectively. A total of 28(13%) clusters and 77(36%) unique cases were identified. Beijing strain (SIT 1) formed the biggest cluster constituting 14%, followed by LAM3 (SIT 33), T1 (SIT 53) and LAM4 (SIT 811) with 7%, 5.1% and 2.8%, respectively. The Beijing family was the only genotype found in all the five districts and was predominant in Mopani (18.8%), Sekhukhune (23.7%) and Vhembe (23.3%). Dominant genotypes in Capricorn and Waterberg were LAM3 (11.9%) and T1 (13.3%), respectively.ConclusionA wide diversity of lineages was demonstrated at district level. A high number of clusters per district provided evidence of on-going transmission in this Province.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Genetic diversity and distribution of Mycobacterium tuberculosis genotypes in Limpopo, South Africa

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The LAM lineage (18.8%) was the subsequent most predominant in our study. This is not surprising considering the fact that this genotype has been discovered to be widespread in Eastern Cape and Free State Provinces [54,55]. This suggests there is a continuous TB transmission strain still ongoing throughout the province.…”

Section: Discussionmentioning

confidence: 90%

Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium Tuberculosis Strains Isolated From Patients in Rural Eastern Cape Province

Faye¹,

Hosu²,

Sineke³

et al. 2023

Preprint

View full text Add to dashboard Cite

Drug-resistant tuberculosis (DR-TB) remains a major public health problem in South Africa. Gene mu-tations and the genotyping of DR-TB in a rural Eastern Cape Province have not previously been studied. Hence, we sought to detect prevalent mutations linked to DR-TB profiles, identify genetic diversity, and assign lineages. Sputum specimens were obtained from 1157 patients suspected with tuberculosis. They were assayed for rifampin-resistance using Xpert® MTB/RIF.and detection of mutations conferring re-sistance to anti-TB drugs was carried out using GenoType MTBDRplus VER 2.0. Thereafter, 441 isolates were spoligotyped. The most prevalent rifampin resistance-conferring mutations were in rpoB codon S531L. The INH resistant strains, 54.5% had mutations in katG gene at codon S315TB and 24.7% with mutations in inhA gene at codons C-15TB. Furthermore 69.9% strains displayed mutations involving both rpoB and katG genes, while 24.6% strains displayed mutations involving both rpoB and inhA genes. The overall prevalence of heteroresistance was 17.9%. Spoligotyping analysis generated 410 patterns of iso-lates which were found in the international database, with Beijing family being predominant. The diversity of mutations in the study provides information for investigating the evolutional lineages of M. tuberculosis isolates. The recognition frequency of rpoB, katG and inhA mutations in different study areas may help to guide decision‑making about standardization of treatment regimens or individualized treatment in areas where these mutations have been found. This calls for increased drug resistance surveillance and rein-forcement of TB control in this area.

show abstract

“…In our investigation, the LAM lineage was the next most common (18.8%) after the Beijing strain. This is not unexpected, given that this genotype has been found to be widespread in the provinces of the Eastern Cape and the Free State [ 62 , 63 ]. This suggests that there is still continuous TB transmission throughout the province.…”

Section: Discussionmentioning

confidence: 94%

The Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium tuberculosis Strains Isolated from Patients in the Rural Eastern Cape Province

Faye

Hosu

Oostvogels

et al. 2023

Infectious Disease Reports

View full text Add to dashboard Cite

Drug-resistant tuberculosis (DR-TB) is still a major public health concern in South Africa. Mutations in M. tuberculosis can cause varying levels of phenotypic resistance to anti-TB medications. There have been no prior studies on gene mutations and the genotyping of DR-TB in the rural Eastern Cape Province; hence, we aimed to identify DR-TB mutations, genetic diversity, and allocated lineages among patients in this area. Using Xpert® MTB/RIF, we assessed the rifampin resistance of sputum samples collected from 1157 patients suspected of having tuberculosis. GenoType MTBDR plus VER 2.0 was used for the detection of mutations causing resistance to anti-TB medications. The next step was to spoligotype 441 isolates. The most prevalent rifampin resistance-conferring mutations were in rpoB codon S531L in INH-resistant strains; the katG gene at codon S315TB and the inhA gene at codon C-15TB had the most mutations; 54.5% and 24.7%, respectively. In addition, 24.6% of strains showed mutations in both the rpoB and inhA genes, while 69.9% of strains showed mutations in both the katG and rpoB genes. Heteroresistance was seen in 17.9% of all cases in the study. According to spoligotyping analysis, Beijing families predominated. Investigation of the evolutionary lineages of M. tuberculosis isolates can be carried out using the information provided by the study’s diversity of mutations. In locations wherein these mutations have been discovered, decision-making regarding the standardization of treatment regimens or individualized treatment may be aided by the detection frequency of rpoB, katG, and inhA mutations in various study areas.

show abstract

Diverse Molecular Genotypes ofMycobacterium tuberculosisComplex Isolates Circulating in the Free State, South Africa

Cited by 8 publications

References 36 publications

Genetic diversity and distribution of Mycobacterium tuberculosis genotypes in Limpopo, South Africa

Genetic diversity and distribution of Mycobacterium tuberculosis genotypes in Limpopo, South Africa

Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium Tuberculosis Strains Isolated From Patients in Rural Eastern Cape Province

The Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium tuberculosis Strains Isolated from Patients in the Rural Eastern Cape Province

Contact Info

Product

Resources

About