Diverse macrophage populations mediate acute lung inflammation and resolution

Aggarwal, Neil R.; King, Landon S.; D’Alessio, Franco R.

doi:10.1152/ajplung.00341.2013

Cited by 483 publications

(435 citation statements)

References 204 publications

(230 reference statements)

Supporting

Mentioning

408

Contrasting

Unclassified

Order By: Relevance

“…Studies of lung regeneration after partial pneumonectomy support the concept that interactions among activated macrophages and lung parenchymal cells create a proliferative microenvironment (22). In the present study, regeneration was associated with marked increase in inflammatory cell expression of ARG1 and other markers of atypical macrophage activation (M2-like), including RETNLA and CD206, proposed to contribute to repair processes (22,41,42). Alveolar macrophages expressing CD206 and ARG1 were identified by immunofluorescence confocal microscopy in both Abca3-cKO mouse lung during regeneration and in lung tissue from patients with severe lung disease caused by ABCA3 mutations.…”

Section: Discussionsupporting

confidence: 71%

Alveolar injury and regeneration following deletion of ABCA3

Rindler¹,

Stockman²,

Filuta³

et al. 2017

JCI Insight

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 71%

Alveolar injury and regeneration following deletion of ABCA3

Rindler¹,

Stockman²,

Filuta³

et al. 2017

JCI Insight

View full text Add to dashboard Cite

“…Because resident immune cells play a critical role in host defense (16)(17)(18)(19)(20), we chose to evaluate the vital functions of immune cells in cryopreserved hPCLSs. We first assessed phagocytosis, a critical process for clearance of infection.…”

Section: Function Of Phagocytes and Lymphocytes In Cryopreserved Hpclssmentioning

confidence: 99%

Cryopreserved Human Precision-Cut Lung Slices as a Bioassay for Live Tissue Banking. A Viability Study of Bronchodilation with Bitter-Taste Receptor Agonists

Bai

Krishnamoorthy

Patel

et al. 2016

Am J Respir Cell Mol Biol

View full text Add to dashboard Cite

Human precision-cut lung slices (hPCLSs) provide a unique ex vivo model for translational research. However, the limited and unpredictable availability of human lung tissue greatly impedes their use. Here, we demonstrate that cryopreservation of hPCLSs facilitates banking of live human lung tissue for routine use. Our results show that cryopreservation had little effect on overall cell viability and vital functions of immune cells, including phagocytes and T lymphocytes. In addition, airway contraction and relaxation in response to specific agonists and antagonists, respectively, were unchanged after cryopreservation. At the subcellular level, cryopreserved hPCLSs maintained Ca 21 -dependent regulatory mechanisms for the control of airway smooth muscle cell contractility. To exemplify the use of cryopreserved hPCLSs in smooth muscle research, we provide evidence that bitter-taste receptor (TAS2R) agonists relax airways by blocking Ca 21 oscillations in airway smooth muscle cells. In conclusion, the banking of cryopreserved hPCLSs provides a robust bioassay for translational research of lung physiology and disease.

show abstract

“…Pathogens escaping this first line of defense are encountered and recognized by the innate immune and scavenger receptors of underlying epithelial cells and macrophages (1). Alveolar macrophages (AMs) provide a crucial defense mechanism against bacterial pathogens in the lower airways, and their phenotype and function often vary between the inflammatory state, the resolution of inflammation, and noninflammatory conditions (2). The M0/M1/M2 paradigm of macrophage activation posits that foreign antigens stimulate a reprogramming of global gene expression resulting in the initial transition of resident M0 macrophages to an M1 phenotype expressing high levels of proinflammatory cytokines and mediating antimicrobial host defense.…”

mentioning

confidence: 99%

Membrane-Tethered MUC1 Mucin Counter-Regulates the Phagocytic Activity of Macrophages

Kato

Uchino

Lillehoj

et al. 2016

Am J Respir Cell Mol Biol

View full text Add to dashboard Cite

MUC1 (MUC in human; Muc in animals) is a transmembrane mucin glycoprotein expressed in mucosal epithelial cells and hematopoietic cells. MUC1 is involved in the resolution of inflammation during airway Pseudomonas aeruginosa (Pa) infection by suppressing Toll-like receptor signaling in airway epithelial cells. Although alveolar macrophages are recognized as critical mediators of cell-mediated immunity against microorganisms inhaled into the airways, the role of MUC1 in regulating their response is unknown. The aims of this study were to determine whether macrophages express MUC1, and, if so, whether MUC1 expression might be associated with macrophage M0/M1/M2 differentiation or phagocytic activity. Human and mouse MUC1/Muc1 expression was drastically up-regulated in classically activated (M1) macrophages compared with nonactivated (M0) and alternatively activated (M2) macrophages. M1 polarization and Pa stimulation each increased MUC1 ectodomain shedding from the macrophage surface in a TNF-a-converting enzyme-dependent manner. MUC1/Muc1 deficiency in M0 macrophages increased adhesion and phagocytosis of Pa and Escherichia coli compared with MUC1/Muc1-expressing cells, and attenuation of phagocytosis by MUC1 was augmented after polarization into M1 macrophages compared with M0 macrophages. Finally, MUC1/Muc1 deficiency in macrophages increased reactive oxygen species production and TNF-a release in response to Pa compared with MUC1/Muc1-sufficient cells. These results indicate that MUC1/Muc1 expression by macrophages is predominantly in the M1 subtype, and that MUC1/Muc1 expression in these cells decreases their phagocytic activity in an antiinflammatory manner.

show abstract

Diverse macrophage populations mediate acute lung inflammation and resolution

Abstract: Aggarwal NR, King LS, D'Alessio FR. Diverse macrophage populations mediate acute lung inflammation and resolution.

Cited by 483 publications

References 204 publications

Alveolar injury and regeneration following deletion of ABCA3

Alveolar injury and regeneration following deletion of ABCA3

Cryopreserved Human Precision-Cut Lung Slices as a Bioassay for Live Tissue Banking. A Viability Study of Bronchodilation with Bitter-Taste Receptor Agonists

Membrane-Tethered MUC1 Mucin Counter-Regulates the Phagocytic Activity of Macrophages

Contact Info

Product

Resources

About