Diverse impact of a probiotic strain,<i> Lacticaseibacillus paracasei </i>Shirota, on peripheral mononuclear phagocytic cells in healthy Japanese office workers: a randomized, double-blind, controlled trial

Abstract

Mononuclear phagocytic cells (MPCs) are classified into monocytes/macrophages and dendritic cells (DCs) based on their functions. Cells of MPCs lineage act as immune modulators by affecting effector cells, such as NK cells, T cells, and B cells. This study aimed to investigate the effects of Lacticaseibacillus paracasei strain Shirota (LcS) ingestion on peripheral MPCs, particularly on their expression of functional cell-surface molecules enhanced in healthy adults. Thus, twelve healthy office workers consumed a fermented milk drink containing 1.0 × 10 11 cfu of LcS (LcS-FM) or a control unfermented milk drink (CM) once a day for 6 weeks. Peripheral blood mononuclear cells (PBMCs) were prepared from blood samples, and immune cells and functional cell-surface molecules were analyzed. We observed remarkable differences in the expression of HLAABC, MICA, CD40, and GPR43 in plasmacytoid DCs (pDCs) between the LcS-FM and CM groups, whereas no difference was found in CD86 or HLA-DR expression. The LcS-FM group exhibited higher CD40 expression in both conventional DCs (cDCs) and monocytes, especially in type 2 conventional DCs (cDC2s) and classical monocytes (cMos); higher percentages of cMos, intermediate monocytes (iMos), and nonclassical monocytes; and higher numbers of cMos and iMos in PBMCs than the CM group. LcS ingestion increased the expression of HLAABC, MICA, CD40, and GPR43 in pDCs and CD40 in cDCs and Mos, particularly cDC2s and cMos. These results suggest that LcS modulates the function of MPCs that may lead to the regulation of immune effector

Optimization of Fermentation and Sensory Quality of Mango Yogurt Using Various Sucrose Concentration Fermented by Lacticaseibaciluus paracasei Strain Shirota

“…Yakult, a fermented milk drink containing a LAB strain, particularly Lacticaseibacillus paracasei strain Shirota (LcS), is known for its ability to resist gastric conditions and colonize the small intestine. This colonization results in the production of antimicrobial substances, stimulating macrophage activity and proliferation (Cook et al, 2023;Naito et al, 2023). Renowned for its health benefits, Yakult is accessible in 15 countries, including various Southeast Asian countries and Indonesia.…”

Lactobacillus paragasseri SBT2055 Activates Plasmacytoid Dendritic Cells and Improves Subjective Symptoms of Common Cold in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled Parallel-Group Comparative Trial

“…Kubo et al showed that lactoferrin and its digestive peptides activated pDCs and induced interferon-α (IFN-α) production ex vivo [6]. Moreover, some non-pathogenic bacteria, such as lactic acid bacteria (LAB), have been reported to activate pDCs in clinical trials [7][8][9][10]. The availability and functionality of LAB are well-known.…”