Diverse human rhinoviruses A and C from children with respiratory infections in Kuala Lumpur, Malaysia

Chan, Yoke Fun; Jafar, Faizatul Lela; Nathan, Anna-Marie; Bruyne, Jessie Anne de; Hassan, Ardalinah; Nor'e, Siti Sarah; AbuBakar, Sazaly; Sam, I‐Ching

doi:10.1016/j.jinf.2012.03.011

Cited by 5 publications

(8 citation statements)

References 13 publications

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“…It is possible that HRV-B was associated with milder disease and therefore had a lower frequency of detection in hospital-based studies. In support of this theory are several other studies which reported low numbers of HRV-B in hospitalized patients [8,18,25,26]. However, a recent study also found a low prevalence of HRV-B in the community [27] suggesting that the low frequency of HRV-B may be related to biological fitness rather than selection bias.…”

Section: Discussionmentioning

confidence: 71%

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Patient characteristics and severity of human rhinovirus infections in children

Lauinger

Bible²,

Halligan³

et al. 2013

Journal of Clinical Virology

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Section: Discussionmentioning

confidence: 71%

“…However, there were recent suggestions that HRV could be associated with severe respiratory tract infections (RTI), acute asthma exacerbations [1,2], recurring wheeze [3] and lower RTI (LRTI) [4][5][6][7]. Dependent on the study, the reported incidence of HRV infections in children ranges from <10% in RTI [8] to 90% in acute asthma [9].…”

Section: Introductionmentioning

confidence: 99%

Patient characteristics and severity of human rhinovirus infections in children

Lauinger

Bible²,

Halligan³

et al. 2013

Journal of Clinical Virology

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“…Studies have revealed that HRV-C has been detected in patients with respiratory tract infections worldwide 1 12 13 14 15 16 17 18 19 . Although the clinical manifestations of HRV-C infections vary in different areas 4 20 , this infection is mainly associated with cough and asthma exacerbations 2 21 , acute lower respiratory tract infections, bronchiolitis, and pneumonia, particularly in children 4 22 .…”

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confidence: 99%

Molecular Epidemiology of Human Rhinovirus in Children with Acute Respiratory Diseases in Chongqing, China

Wang

et al. 2014

Sci Rep

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Human rhinovirus-C (HRV-C) has been increasingly detected in patients with acute respiratory diseases (ARDs). Prolonged surveillance was performed on children with ARD to investigate the molecular epidemiology and clinical characteristics of HRV in Chongqing, China. Nasopharyngeal aspirates (NPA) were collected from hospitalized children with ARD during 2009–2012. HRV-C was genotyped by sequencing the VP4/VP2 coding region. Among the 1,567 NPAs obtained, 223 (14.2%) were HRV positive, and 75.3% of these 223 NPAs were co-infected with other viruses. HRV-A (54.7%) and HRV-C (39.9%) accounted for the majority of HRV infections. Logistic regression models demonstrated significant associations between HRV-A, HRV-C, and asthma attacks, as well as between HRV-C and wheezing. A phylogenetic tree showed that HRV-C2 was the predominant type of HRV-C, followed by HRV-C43, HRV-C1, and HRV-C17. Three novel genotypes were proposed on the basis of a low identity with the known HRVs. Our results showed that HRV-A and HRV-C were the predominant types of HRV infection, and HRV-C showed a high genetic variation in Chongqing, China. HRV infection was associated with asthma attacks and wheezing; furthermore, HRV infections played a minor role in causing severe pneumonia. This knowledge provides information for the prevention and control of HRV associated with ARDs.

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“…Seven nasopharyngeal aspirate (NPA) samples were collected from children with respiratory tract infection at University Malaya Medical Center (UMMC) in Malaysia between October 2010 and May 2011 (Medical Ethic Approval no. 788.3) (Chan et al, 2012 ). These samples were previously confirmed as HRV-C based on the sequence of the VP4/VP2 region (Chan et al, 2012 ).…”

Section: Methodsmentioning

confidence: 99%

“…788.3) (Chan et al, 2012 ). These samples were previously confirmed as HRV-C based on the sequence of the VP4/VP2 region (Chan et al, 2012 ). RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen, Germany) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Comparative Genetic Analyses of Human Rhinovirus C (HRV-C) Complete Genome from Malaysia

et al. 2016

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Human rhinovirus-C (HRV-C) has been implicated in more severe illnesses than HRV-A and HRV-B, however, the limited number of HRV-C complete genomes (complete 5′ and 3′ non-coding region and open reading frame sequences) has hindered the in-depth genetic study of this virus. This study aimed to sequence seven complete HRV-C genomes from Malaysia and compare their genetic characteristics with the 18 published HRV-Cs. Seven Malaysian HRV-C complete genomes were obtained with newly redesigned primers. The seven genomes were classified as HRV-C6, C12, C22, C23, C26, C42, and pat16 based on the VP4/VP2 and VP1 pairwise distance threshold classification. Five of the seven Malaysian isolates, namely, 3430-MY-10/C22, 8713-MY-10/C23, 8097-MY-11/C26, 1570-MY-10/C42, and 7383-MY-10/pat16 are the first newly sequenced complete HRV-C genomes. All seven Malaysian isolates genomes displayed nucleotide similarity of 63–81% among themselves and 63–96% with other HRV-Cs. Malaysian HRV-Cs had similar putative immunogenic sites, putative receptor utilization and potential antiviral sites as other HRV-Cs. The genomic features of Malaysian isolates were similar to those of other HRV-Cs. Negative selections were frequently detected in HRV-Cs complete coding sequences indicating that these sequences were under functional constraint. The present study showed that HRV-Cs from Malaysia have diverse genetic sequences but share conserved genomic features with other HRV-Cs. This genetic information could provide further aid in the understanding of HRV-C infection.

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Diverse human rhinoviruses A and C from children with respiratory infections in Kuala Lumpur, Malaysia

Cited by 5 publications

References 13 publications

Patient characteristics and severity of human rhinovirus infections in children

Patient characteristics and severity of human rhinovirus infections in children

Molecular Epidemiology of Human Rhinovirus in Children with Acute Respiratory Diseases in Chongqing, China

Comparative Genetic Analyses of Human Rhinovirus C (HRV-C) Complete Genome from Malaysia

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