Diverse functions of uterine proteoglycans in human reproduction (Review)

Kitaya, Kotaro; Tada, Yoshihiro; Hayashi, Terumi; Taguchi, Sagiri; Funabiki, Miyako; Nakamura, Yoshitaka; Yasuo, Tadahiro

doi:10.3892/mmr.2012.826

Cited by 4 publications

(4 citation statements)

References 49 publications

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“…The cell surface heparan sulfate proteoglycan CD138 (also known as syndecan-1) has been utilized in the diagnosis of plasmacytoma as a specific and sensitive marker of bone marrow and circulating blood malignant plasmacytes (6). Immunohistochemistry for CD138 has also been observed to be suitable for the detection of endometrial stromal plasmacytes in paraffin-embedded specimens and improves the diagnosis rate of CE (1,7,8). …”

Section: Introductionmentioning

confidence: 99%

Inter-observer and intra-observer variability in immunohistochemical detection of endometrial stromal plasmacytes in chronic endometritis

Kitaya

Yasuo

2012

Experimental and Therapeutic Medicine

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Chronic endometritis (CE) is an unusual endometrial inflammation characterized by stromal plasmacyte infiltration. CE is easily missed due to its subtle symptoms and demanding histopathological examinations. Although the immunohistochemistry for the plasmacyte marker CD138 has facilitated the detection of endometrial stromal plasmacytes, the accuracy and biases of this method for CE diagnosis remain poorly understood. The aim of this study was to investigate the inter- and intra-observer variability in the immunohistochemical detection of stromal plasmacytes in the human endometrium. A total of 80 CE and 20 non-pathological archival hematoxylin-stained endometrial preparations with or without immunostaining for CD138 were evaluated independently by two experienced observers and two inexperienced observers. Endometrial stromal plasmacytes in unit areas were counted in the hematoxylin-stained and CD138-immunostained preparations. Each preparation was subdivided into 11 categories by every five plasmacyte counts. The second evaluation was performed four weeks after the first evaluation. The immunohistochemical detection method was superior to conventional histopathological evaluation in both the inter- and intra-observer agreement, irrespective of the experience level of the observers. The linear weighted κ coefficient for intra-observer agreement was higher in the experienced observers than in the inexperienced observers. The inter-observer agreement among the four observers by the immunohistochemical detection method was similarly good between the first and second evaluation. There was no significant inter- or intra-observer variability in the paired comparison of the individual samples. These findings validate the use of immunohistochemistry for CD138 as an accurate and less biased diagnostic tool for CE.

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Section: Introductionmentioning

confidence: 99%

Inter-observer and intra-observer variability in immunohistochemical detection of endometrial stromal plasmacytes in chronic endometritis

Kitaya

Yasuo

2012

Experimental and Therapeutic Medicine

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“…In spite of extensive information on the expression and functions of proteoglycans in the reproductive organs (animal models), the exact role of these molecules in human reproductive organs has not been determined (Kitaya et al. ). In this sense, our study confirms the presence and differential expression of acid proteoglycans by the Alcian blue histochemical method and, more specifically, by immunohistochemistry.…”

Section: Discussionmentioning

confidence: 99%

Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis

Godoy-Guzmán

Nuñez²,

Orihuela

et al. 2018

Journal of Anatomy

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The uterine tube (UT) is an important and complex organ of the women's reproductive system. In general, the anatomy and basic histology of this organ are well-known. However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood. The ECM is a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces. ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues. In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques. Our results showed a broad synthesis of collagens (I and III) in the stroma of the UT. The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria. Versican and decorin were found in the stroma of the muscular layer, whereas all studied proteoglycans were identified in the serosa. Curiously, biglycan was restricted to the wall of the blood vessels of the serosa and muscular layers. Furthermore, there was an immunoreaction for collagens, decorin, versican and fibromodulin in the UT peripheral nerves. The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis. However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.

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“…In the female reproductive tract CXCL1 is expressed throughout the cycling endometrium, mainly in the stroma with increasing amounts during the secretory phase, and particularly decidualization enhances the expression of CXCL1 in EnS cells [ 42 ]. Sdc-1, the coreceptor of CXCL1, is a heparan sulfate proteoglycan cell surface molecule which is localized on endometrial stromal cells, myometrium under pregnant conditions, placenta, and amniotic epithelium [ 20 , 43 , 44 ]. Known signaling pathways involved in the human implantation process are the MAPK and Wnt pathway, both playing roles in differentiation, proliferation, angiogenesis, and apoptosis at the embryo-maternal interface [ 2 , 13 ].…”

Section: Discussionmentioning

confidence: 99%

Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

Baston-Buest

Altergot-Ahmad

Pour

et al. 2017

Mediators of Inflammation

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Successful implantation of the embryo into the human receptive endometrium is substantial for the establishment of a healthy pregnancy. This study focusses on the role of Syndecan-1 at the embryo-maternal interface, the multitasking coreceptor influencing ligand concentration, release and receptor presentation, and cellular morphology. CXC motif ligand 1, being involved in chemotaxis and angiogenesis during implantation, is of special interest as a ligand of Syndecan-1. Human endometrial stromal cells with and without Syndecan-1 knock-down were decidualized and treated with specific inhibitors to evaluate signaling pathways regulating CXC ligand 1 expression. Western blot analyses of MAPK and Wnt members were performed, followed by analysis of spheroid interactions between human endometrial cells and extravillous trophoblast cells. By mimicking embryo contact using IL-1β, we showed less ERK and c-Jun activation by depletion of Syndecan-1 and less Frizzled 4 production as part of the canonical Wnt pathway. Additionally, more beta-catenin was phosphorylated and therefore degraded after depletion of Syndecan-1. Secretion of CXC motif ligand 1 depends on MEK-1 with respect to Syndecan-1. Regarding the interaction of endometrial and trophoblast cells, the spheroid center-to-center distances were smaller after depletion of Syndecan-1. Therefore, Syndecan-1 seems to affect signaling processes relevant to signaling and intercellular interaction at the trophoblast-decidual interface.

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Diverse functions of uterine proteoglycans in human reproduction (Review)

Cited by 4 publications

References 49 publications

Inter-observer and intra-observer variability in immunohistochemical detection of endometrial stromal plasmacytes in chronic endometritis

Inter-observer and intra-observer variability in immunohistochemical detection of endometrial stromal plasmacytes in chronic endometritis

Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis

Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

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