Diverse expression pattern of wheat transcription factors against abiotic stresses in wheat species

Baloğlu, Mehmet Cengiz; İnal, Behçet; Kavas, Musa; Ünver, Turgay

doi:10.1016/j.gene.2014.08.025

Cited by 46 publications

(26 citation statements)

References 42 publications

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“…Furthermore, differences in expression level of VfWRKY1 and VfWRKY2 in drought-tolerant (Hara) and -sensitive (Giza 3) cultivars could be linked to their response to drought and salt stress. Interestingly, the current data are in accordance with other studies, which found that in T. aestivum, a drought-tolerant cultivar exhibited a higher extent of up-regulation of TaWRKY10 gene in response to drought and salt stress (Baloglu et al 2014).…”

Section: Discussionsupporting

confidence: 93%

Identification and characterization of two faba bean (Vicia faba L.) WRKY transcription factors and their expression analysis during salt and drought stress

et al. 2016

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SUMMARYDrought and salinity are two major environmental factors limiting faba bean growth, leading to considerable reduction in their productivity. The WRKY gene family act as major transcription factors that might play an important role in abiotic stress tolerance. In the present study, two partial sequences sharing significant homology with known WRKY genes were isolated from faba bean by polymerase chain reaction (PCR) amplification using degenerate primers targeting the well-conserved WRKY domain. The isolated WRKY gene fragments were designated as VfWRKY1 and VfWRKY2 showing 62% similarity between them. Sequence and phylogenetic analyses revealed that VfWRKY1 and VfWRKY2 belong to WRKY group I and could be grouped with their orthologues from other plant species. The gene expression profile of VfWRKY1 and VfWRKY2 in faba bean showed that they are significantly accumulated in various plant organs. Further, quantitative real-time PCR analysis showed that both transcripts were responsive to drought and salt stress, and also they are genotype dependent, meaning that different faba bean cultivars respond in a different way to drought and salt challenge. The expression patterns obtained suggest the important roles of VfWRKY1 and VfWRKY2 in drought and salt stress response and tolerance. This knowledge might be helpful in the identification of drought-tolerant cultivars and provide potential candidate markers for faba bean breeding in order to develop osmotic-stress-tolerant cultivars.

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Section: Discussionsupporting

confidence: 93%

Identification and characterization of two faba bean (Vicia faba L.) WRKY transcription factors and their expression analysis during salt and drought stress

et al. 2016

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“…The fold transcript abundance (also referred to as the induction ratio in some published work) was calculated as recommended by the manufacturer and corresponds to 2 −ΔΔC T , where ΔΔCT = (C T , Target gene – C T , 18S) stressed – (C T , Target gene – C T , 18S) control . The 18S gene, as well as other genes encoding for rRNAs, is widely used as a reference for quantitative reverse transcription (qRT)–PCR in durum wheat as reported in several recent papers (Ciaffi et al ; Baloglu et al ). Moreover, to show that the use of random primers for cDNA synthesis and subsequent 18S quantification does not differ from the use of oligo‐dT, we made the control using either oligo‐dT or random primers for cDNA synthesis and quantification of transcript abundance of the 18S gene and of some of the genes considered in the paper.…”

Section: Methodsmentioning

confidence: 99%

Gene expression and physiological responses to salinity and water stress of contrasting durum wheat genotypes

Yousfi

Márquez

Betti

et al. 2015

JIPB

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Elucidating the relationships between gene expression and the physiological mechanisms remains a bottleneck in breeding for resistance to salinity and drought. This study related the expression of key target genes with the physiological performance of durum wheat under different combinations of salinity and irrigation. The candidate genes assayed included two encoding for the DREB (dehydration responsive element binding) transcription factors TaDREB1A and TaDREB2B, another two for the cytosolic and plastidic glutamine synthetase (TaGS1 and TaGS2), and one for the specific Na(+) /H(+) vacuolar antiporter (TaNHX1). Expression of these genes was related to growth and different trait indicators of nitrogen metabolism (nitrogen content, stable nitrogen isotope composition, and glutamine synthetase and nitrate reductase activities), photosynthetic carbon metabolism (stable carbon isotope composition and different gas exchange traits) and ion accumulation. Significant interaction between genotype and growing conditions occurred for growth, nitrogen content, and the expression of most genes. In general terms, higher expression of TaGS1, TaGS2, TaDREB2B, and to a lesser extent of TaNHX1 were associated with a better genotypic performance in growth, nitrogen, and carbon photosynthetic metabolism under salinity and water stress. However, TaDREB1A was increased in expression under stress compared with control conditions, with tolerant genotypes exhibiting lower expression than susceptible ones.

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“…In Northwest China, wheat is cultivated under rain-fed conditions and encounters severe water shortage because of the low rainfall [4]. Uncovering the sophisticated underlying mechanisms that allow wheat to adapt to various detrimental stresses has vital significance for the improvement of wheat drought tolerance [5,6]. Several transcription factor (TF) families, such as WRKY, MYB, and NAC, have been reported to play key roles in response to drought stress in plants.…”

Section: Introductionmentioning

confidence: 99%

A Wheat WRKY Transcription Factor TaWRKY46 Enhances Tolerance to Osmotic Stress in transgenic Arabidopsis Plants

Yan

Zhou

et al. 2020

IJMS

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WRKY transcription factors play central roles in developmental processes and stress responses of wheat. Most WRKY proteins of the same group (Group III) have a similar function in abiotic stress responses in plants. TaWRKY46, a member of Group III, was up-regulated by PEG treatment. TaWRKY46-GFP fusion proteins localize to the nucleus in wheat mesophyll protoplasts. Overexpression of TaWRKY46 enhanced osmotic stress tolerance in transgenic Arabidopsis thaliana plants, which was mainly demonstrated by transgenic Arabidopsis plants forming higher germination rate and longer root length on 1/2 Murashige and Skoog (MS) medium containing mannitol. Furthermore, the expression of several stress-related genes (P5CS1, RD29B, DREB2A, ABF3, CBF2, and CBF3) was significantly increased in TaWRKY46-overexpressing transgenic Arabidopsis plants after mannitol treatment. Taken together, these findings proposed that TaWRKY46 possesses vital functions in improving drought tolerance through ABA-dependent and ABA-independent pathways when plants are exposed to adverse osmotic conditions. TaWRKY46 can be taken as a candidate gene for transgenic breeding against osmotic stress in wheat. It can further complement and improve the information of the WRKY family members of Group III.

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Diverse expression pattern of wheat transcription factors against abiotic stresses in wheat species

Cited by 46 publications

References 42 publications

Identification and characterization of two faba bean (Vicia faba L.) WRKY transcription factors and their expression analysis during salt and drought stress

Identification and characterization of two faba bean (Vicia faba L.) WRKY transcription factors and their expression analysis during salt and drought stress

Gene expression and physiological responses to salinity and water stress of contrasting durum wheat genotypes

A Wheat WRKY Transcription Factor TaWRKY46 Enhances Tolerance to Osmotic Stress in transgenic Arabidopsis Plants

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