Diverse effects of essential (n-6 and n-3) fatty acids on cultured cells

Grammatikos, Stephanos I.; Subbaiah, Papasani V.; Victor, Thomas A.; Miller, William M.

doi:10.1007/978-94-011-0257-5_5

Cited by 15 publications

(18 citation statements)

References 106 publications

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“…One of these effects can be caused by altering the membrane fluidity and thus affecting any process that is mediated via the membrane (Grammatikos et al 1994a). In this context, inhibition of oocyte maturation in LA-treated COCs may be mediated by altering the cumulus cell membrane response to gonadotrophin stimulation.…”

Section: Discussionmentioning

confidence: 99%

Impact of linoleic acid on bovine oocyte maturation and embryo development

Marei¹,

Wathes²,

2010

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Linoleic acid (LA; 18:2 n-6) is the most abundant fatty acid in bovine follicular fluid, and it was previously reported that LA concentration significantly decreases when follicle size increases. This suggests that LA may have a role in the regulation of oocyte maturation. The present study investigated the effect of LA supplementation on bovine oocyte maturation and early embryo development in vitro. Treatment of cumulus-oocyte complexes (COCs) with LA significantly inhibited cumulus cell expansion and retarded development of the oocytes to the metaphase II (MII) stage in a dose-dependent manner. This effect was reversible, and the oocytes developed to the MII stage after extended culture in the absence of LA. Treatment of COCs with LA also resulted in a significantly lower percentage of cleaved embryos and blastocyst yield. Furthermore, COCs treated with LA had significant effects compared with controls in i) increasing prostaglandin E 2 concentration in the medium, ii) decreasing intracellular cAMP at 6 and 24 h of maturation and iii) decreasing phosphorylation of the MAPK1 and 3 at 24 h, and AKT at 6 h of maturation. In conclusion, LA supplementation to bovine oocytes during maturation altered the molecular mechanisms regulating oocyte maturation and resulted in decreased percentage of oocytes at MII stage and inhibition of the subsequent early embryo development. These data provide evidence for adverse effects of LA on oocyte development, which can be associated with dietary increased level of LA in the follicular fluid and the decline in fertility in farm animals and human.Reproduction (2010) 139 979-988

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Section: Discussionmentioning

confidence: 99%

Impact of linoleic acid on bovine oocyte maturation and embryo development

Marei¹,

Wathes²,

2010

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“…The increase in the MDA level is an important sign of oxidative stress (Kim et al 2006). The MDA levels are determined as an index of LPO and LPO is one of the main manifestations of the toxicity and carcinogenesis of many carcinogens (Grammatikos et al 1994;Rastogi et al 2001;Siu et al 2008). The marked increases in MDA content after AFB 1 -exposure could contribute to the effect of AFB 1 on antioxidant defense system.…”

Section: Discussionmentioning

confidence: 99%

Boric acid: a potential chemoprotective agent against aflatoxin b1 toxicity in human blood

Türkez

Geyikoğlu

2010

Cytotechnology

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Aflatoxin B 1 is the most potent pulmonary and hepatic carcinogen. Since the eradication of Aflatoxin B 1 contamination in agricultural products has been difficult, the use of natural or synthetic free radical scavengers could be a potential chemopreventive strategy. Boric acid is the major component of industry and its antioxidant role has recently been reported. The present study assessed, for the first time, the effectiveness of boric acid following exposure to Aflatoxin B 1 on human whole blood cultures. The biochemical characterizations of glutathione and some enzymes have been carried out in erythrocytes. Alterations in malondialdehyde level were determined as an index of oxidative stress. The sister-chromatid exchange and micronucleus tests were performed to assess DNA damages in lymphocytes. Aflatoxin B 1 treatment significantly reduced the activities of antioxidants by increasing malondialdehyde level (30.53 and 51.43%) of blood, whereas, the boric acid led to an increased resistance of DNA to oxidative damage induced by Aflatoxin B 1 in comparison with control values (P \ 0.05). In conclusion, the support of boric acid was especially useful in Aflatoxin-toxicated blood. Thus the risk on tissue targeting of Aflatoxin B 1 could be reduced ensuring early recovery from its toxicity.

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“…The effects of n-3 PUFA derived from marine oils on cell growth have previously been investigated using a variety of cultured tumor cell lines, including those derived from mammary (19,20) prostate (21), pancreatic (22,23), cervical (24), and colonic tissue (25). On the basis of this work two major theories have been proposed to account for the inhibition of tumor growth by n-3 PUFA.…”

mentioning

confidence: 98%

“…On the basis of this work two major theories have been proposed to account for the inhibition of tumor growth by n-3 PUFA. The first is that increased availability of n-3 PUFA modifies the lipoxygenase and cyclooxygenase pathways for eicosanoid synthesis so as to reduce the rate of cell proliferation (19,26). The second is that incorporation of n-3 PUFA into the membranes of cells increases their susceptibility to oxidative damage (27)(28)(29), and hence to programmed cell death (apoptosis).…”

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confidence: 99%

Effect of eicosapentaenoic acid on the proliferation and incidence of apoptosis in the colorectal cell line HT29

Clarke

Lund

Latham

et al. 1999

Lipids

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Fish oil has been shown to reduce the induction of colorectal cancer in animal models by a mechanism which may involve suppression of mitosis, increased apoptosis, or both. We used the human colonic adenocarcinoma cell line HT29 to explore the effects of the long-chain n-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) on cell proliferation and death in vitro. Cells were cultured in media containing EPA at 5, 10, and 15 microg/mL. Cell number and thymidine incorporation were used to quantify proliferation, and cell cycle effects were studied using flow cytometry. Gel electrophoresis, annexin-V binding, and morphological criteria were used to characterize apoptosis. Adherent cells and freely floating detached cells were treated as two distinct populations. In the presence of EPA at 10 and 15 microg/mL there was a marked reduction in the growth rate of adherent HT29 colonies, owing to an increased detachment of adherent cells. After treatment with 10 or 15 microg/mL EPA the proportion of adherent cells in S-phase increased, indicating either a block in late S-phase or early G2. Floating cells showed evidence of extensive DNA cleavage, but the proportion of floating cells with sub GO DNA content declined on treatment with 10 or 15 microg/mL EPA even though the number of floating cells increased. We conclude that EPA does not inhibit mitosis of adherent cells, but increases the rate at which they become detached from the substrate, probably at an early stage in the initiation of apoptosis. This mechanism may be analogous to "anoikis," or induction of apoptosis in response to loss of cell contact, and may contribute to the anticarcinogenic effects of fish oil in vivo.

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Diverse effects of essential (n-6 and n-3) fatty acids on cultured cells

Cited by 15 publications

References 106 publications

Impact of linoleic acid on bovine oocyte maturation and embryo development

Impact of linoleic acid on bovine oocyte maturation and embryo development

Boric acid: a potential chemoprotective agent against aflatoxin b1 toxicity in human blood

Effect of eicosapentaenoic acid on the proliferation and incidence of apoptosis in the colorectal cell line HT29

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