Diverse cellular transformation capability of overexpressed genes in human hepatocellular carcinoma

Huang, Jian-Jia; Chao, Chuan-Chuan; Su, Teh-Li; Yeh, Shiou–Hwei; Chen, Ding‐Shinn; Chen, Chiung‐Tong; Chen, Pei‐Jer; Jou, Yuh–Shan

doi:10.1016/j.bbrc.2004.01.151

Cited by 137 publications

(107 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In accordance with our study elevated CLIC1 levels have been reported in hepatocellular carcinoma (Huang et al, 2004) and gastric carcinoma (Chen et al, 2007) and in gastric cancer this was strongly correlated with lymph node metastasis, lymphatic invasion, perineural invasion and pathological staging indicating that CLIC1 could be a potential prognostic marker (Chen et al, 2007). Otherwise, the literature contains little data on this protein and its relationship to cancer.…”

Section: Other Proteinssupporting

confidence: 63%

Differential tissue-specific protein markers of vaginal carcinoma

et al. 2009

View full text Add to dashboard Cite

The objective was to identify proteins differentially expressed in vaginal cancer to elucidate relevant cancer-related proteins. A total of 16 fresh-frozen tissue biopsies, consisting of 5 biopsies from normal vaginal epithelium, 6 from primary vaginal carcinomas and 5 from primary cervical carcinomas, were analysed using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry. Of the 43 proteins identified with significant alterations in protein expression between non-tumourous and tumourous tissue, 26 were upregulated and 17 were downregulated. Some were similarly altered in vaginal and cervical carcinoma, including cytoskeletal proteins, tumour suppressor proteins, oncoproteins implicated in apoptosis and proteins in the ubiquitin -proteasome pathway. Three proteins were uniquely altered in vaginal carcinoma (DDX48, erbB3-binding protein and biliverdin reductase) and five in cervical carcinoma (peroxiredoxin 2, annexin A2, sarcomeric tropomyosin kappa, human ribonuclease inhibitor and prolyl-4-hydrolase beta). The identified proteins imply involvement of multiple different cellular pathways in the carcinogenesis of vaginal carcinoma. Similar protein alterations were found between vaginal and cervical carcinoma suggesting common tumourigenesis. However, the expression level of some of these proteins markedly differs among the three tissue specimens indicating that they might be useful molecular markers.

show abstract

Section: Other Proteinssupporting

confidence: 63%

Differential tissue-specific protein markers of vaginal carcinoma

et al. 2009

View full text Add to dashboard Cite

show abstract

“…However, its association with tumour is less obvious. Transcripts encoding 2 other antigens with cancer-related serological profiles, HSPCA and galectin 4, have been reported to be overexpressed in 45% (20 of 45) (Huang et al, 2004b) and 80% (four of five) (Kondoh et al, 1999) in HCC patients, respectively; their upregulation in HCC may associate with occurrence and progression of tumour. As regulator for premRNA splicing, overexpression of RNPC2 and SR140 protein may also be of aetiological significance in HCC.…”

Section: Discussionmentioning

confidence: 99%

“…Sequencing analysis showed that the 52 clones represented 30 distinct antigens (Table 1). Of these 30 antigens, one is the CT antigen CAGE protein, and the other 13 antigens, which had previously been reported to be involved in tumorigenesis or suspected to be involved in tumour progression, including CDC37 (Stepanova et al, 2000), MIF (Li et al, 2004), galectin 4 (Huflejt and Leffler, 2004), galectin 8 (Bidon-Wagner and Le Pennec, 2004), PINCH (WangRodriguez et al, 2002), SPRY2 (Lee et al, 2004a), HSPCA (Becker et al, 2004;Huang et al, 2004b), transgelin 2 (Shields et al, 2002), HDAC2 , H factor (Junnikkala et al, 2000), AAT (Huang et al, 2004a), B factor (Perou et al, 1999), PIBF (Lachmann et al, 2004). Three other antigens (SR140 protein, SFRS2IP, RNPC2) may be involved in the regulation of alternative mRNA splicing, PSMA7 is related to hepatitis B and hepatitis C viral replication (Zhang et al, 2000;Kruger et al, 2001), and the remaining 12 antigens have no known association with cancer or hepatitis B or hepatitis C.…”

Section: Serex Defined Hcc-associated Antigensmentioning

confidence: 99%

“…The mRNA expression levels of nine antigens, CAGE, transgelin2, HDAC2, RNPC2, PSMD1, HSPCA, PSMA7, U2-associated SR140 protein and galectin 4, were upregulated in HCC, whereas those of the other five antigens, HIMAP4, H factor, C5, AAT and B factor, were downregulated. To validate the cDNA microarray results, with the exception of CAGE, as well as HSPCA and galectin 4, which have been already reported to be overexpressed in HCC (Huang et al, 2004b;Huflejt and Leffler, 2004), the mRNA expression levels of seven antigens out of the remaining 11 antigens were further measured by real-time quantitative RT -PCR. Altered mRNA expression was defined as two-fold differences in the expression level in HCC relative to paired adjacent noncancerous tissue.…”

Section: Expression Patterns Of the Mrna Transcripts Encoding Tumour mentioning

confidence: 99%

See 1 more Smart Citation

Identification and analysis of tumour-associated antigens in hepatocellular carcinoma

Wang

et al. 2005

Br J Cancer

View full text Add to dashboard Cite

To identify tumour and tumour-associated antigens in patients with hepatocellular carcinoma (HCC) one may find potential diagnostic markers and immunotherapeutic targets. In the current study, 30 distinct antigens reactive with serum IgG from HCC patients were identified by serological analysis of cDNA expression libraries (SEREX). The mRNA expression patterns of 14 of these 30 antigens were altered in cancer as further revealed by cDNA microarray, with upregulation for nine and downregulation for five antigens. One of the upregulated antigens was cancer-testis (CT) antigen (CAGE), which had been previously reported to be expressed exclusively in normal gametogenic tissues and aberrantly expressed in a variety of cancer cells. In our study, CAGE mRNA was expressed in 39.4% of HCC patients, 73.3% of patients with gastric cancer and 30.8% of patients with colorectal cancer. Antibodies against CAGE protein were detected in approximately 5.1% of the sera from HCC patients, 8.3% of that from gastric cancer patients and 7.3% of that from colorectal cancer patients. The relative high incidence of CAGE in cancer cells makes it a potential target for vaccine design. Another antigen of great interest is transgelin 2. The overexpression of transgelin 2 mRNA in a large per cent (69%) of HCC points to its potential as a diagnostic marker for HCC.

show abstract

“…Both ischaemia and heat shock inhibit overall levels of protein synthesis. A recent report (Huang et al, 2004) demonstrated over-expression of Trip1 in a variety of hepatic carcinoma cell lines.…”

Section: Trip1mentioning

confidence: 99%

Representational difference analysis of cDNA identifies novel genes expressed following preconditioning of the heart

Fauchon

Pell²,

Baxter³

et al. 2005

Exp Mol Med

View full text Add to dashboard Cite

Preconditioning of the myocardium rapidly induces a number of transcription factors, which are likely to be responsible for a cascade of transcriptional changes underlying the development of delayed adaptation. Identifying these changes provides insight into the molecular pathways elicited by sub-lethal ischaemia and the mechanism leading to delayed adaptation. Genes up-regulated in rabbit myocardium in vivo by ischaemic preconditioning following reperfusion for 2 h, 4 h and 6 h posttreatment were identified by representational difference analysis of cDNA (cDNA. RDA). The area of the left ventricle rendered ischaemic by preconditioning or the equivalent area of sham-treated animals was isolated and cDNA.RDA performed. Three novel genes and six genes with known function where identified, including the TGFβ receptor interacting protein 1, the α isoform of the A subunit of PP2 and the cap binding protein NCBP1. To determine whether expression of these genes correlated with preconditioning per se, expression was measured in myocardium after both ischaemic as well as heat shock induced preconditioning following 2 h, 4 h, and 6 h reperfusion. These genes were induced in rabbit myocardium in vivo by both ischaemia and heat shock, consistent with a fundamental role in the development of delayed adaptation. The well described role of PP2 in modulating the mitogen-activated protein kinase pathway and promoting cell survival is consistent with our previous work, which identified the reperfusion injury salvage kinase pathway in mediating the protective effects of ischaemic preconditioning. Expression of Trip1 and Ncbp1 also implicates TGFβ signalling pathways and RNA processing and transport in delayed adaptation to stress in the myocardium.

show abstract

Diverse cellular transformation capability of overexpressed genes in human hepatocellular carcinoma

Cited by 137 publications

References 41 publications

Differential tissue-specific protein markers of vaginal carcinoma

Differential tissue-specific protein markers of vaginal carcinoma

Identification and analysis of tumour-associated antigens in hepatocellular carcinoma

Representational difference analysis of cDNA identifies novel genes expressed following preconditioning of the heart

Contact Info

Product

Resources

About