Divergent Gemycircularvirus in HIV-Positive Blood, France

Uch, Rathviro; Fournier, Pierre‐Edouard; Robert, Caroline; Blanc-Tailleur, Caroline; Galicher, Vital; Barre, Romain; Jordier, François; Micco, Philippe de; Raoult, Didier; Biagini, Philippe

doi:10.3201/eid2111.150486

Cited by 29 publications

(28 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other gemycircularvirus genomes have been detected in a fungi‐infected plant, sewage, and the bodies of insects as well as mammalian feces and tissues . More recently gemycircularviruses were described from human blood from an HIV‐positive patient, human cerebral spinal fluid, and feces, although these genomes were highly divergent from DB1 and DB2. The detection of gemycircularvirus genomes in plasma pools may therefore potentially reflect contamination of the plasma pools from various sources.…”

Section: Discussionmentioning

confidence: 99%

“…Beside known human viruses, we also detected two gemycircularvirus genomes in the same plasma pool. Gemycircularviruses are genetically highly diverse and have been found in a wide variety of sources but their tropism remain largely unknown except for one virus replicating in fungi (SsHADV‐1 or sclerotinia sclerotiorum hypovirulence‐associated DNA virus 1) …”

mentioning

confidence: 99%

See 1 more Smart Citation

Viral nucleic acids in human plasma pools

Zhang

Deng

et al. 2016

Transfusion

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Viral nucleic acids in human plasma pools

Zhang

Deng

et al. 2016

Transfusion

View full text Add to dashboard Cite

show abstract

“…Particle‐protected nucleic acids were recovered using the High Pure Viral Nucleic Acid Large Volume Kit (Roche Diagnostics) and resuspended in 30 μL ultrapure water. Extracted DNA/RNA templates were then converted into double‐stranded DNA using combined reverse transcription and 2‐hour Phi‐29 DNA polymerase treatments …”

Section: Methodsmentioning

confidence: 99%

Human pegivirus isolates characterized by deep sequencing from hepatitis C virus‐RNA and human immunodeficiency virus‐RNA–positive blood donations, France

Jordier

Deligny

Barre

et al. 2018

Journal of Medical Virology

Self Cite

View full text Add to dashboard Cite

Human pegivirus (HPgV, formerly GBV-C) is a member of the genus Pegivirus, family Flaviviridae. Despite its identification more than 20 years ago, both natural history and distribution of this viral group in human hosts remain under exploration. Analysis of HPgV genomes characterized up to now points out the scarcity of French pegivirus sequences in databases. To bring new data regarding HPgV genomic diversity, we investigated 16 French isolates obtained from hepatitis C virus-RNA and human immunodeficiency virus-RNA-positive blood donations following deep sequencing and coupled molecular protocols. Initial phylogenetic analysis of 5'-untranslated region (5'-UTR)/E2 partial sequences permitted to assign HPgV isolates to genotypes 2 (n = 15) and 1 (n = 1), with up to 16% genetic diversity observed for both regions considered. Seven nearly full-length representative genomes were characterized subsequently, with complete polyprotein coding sequences exhibiting up to 13% genetic diversity; closest nucleotide (nt) divergence with available HPgV sequences was in the range 7% to 11%. A 36 nts deletion located on the NS4B coding region (N-terminal part, 12 amino acids) of the genotype 1 HPgV genome characterized was identified, along with single nucleotide deletions in two genotype 2, 5'-UTR sequences.

show abstract

“…For example, the ancient caribou feces-associated virus (aCFV), which was discovered in 700-y-old caribou feces, could infect Nicotiana benthamiana (23). Gemycircularviruses, which were detected in samples of human blood, serum, cerebrospinal fluids, and brain (28,29), as well as horse liver and spleen (31), most likely replicate and are retained in human and animal cells, suggesting that humans and animals are possible hosts of these viruses. The finding that gemycircularviruses were detected in mosquitoes suggests that mosquitoes and other parasitic insects such as fleas as well as parasitic mites and ticks may serve as vectors for transmission of human and animal gemycircularviruses.…”

Section: Discussionmentioning

confidence: 99%

“…The DNA of SsHADV-1-like viruses was detected in a microbial community from the Sargasso Sea (18) and was found in fecal samples of unexplained human diarrhea (20) and in various animal feces (21)(22)(23), insects (such as mosquitoes, dragonflies, and damselflies) (19,(24)(25)(26), and plants (27). SsHADV-1-like viral DNA was found in human brain and serum samples from multiple sclerosis patients (28), in human HIV-positive blood (29), in the cerebrospinal fluids of Sri Lankan patients with unexplained encephalitis (20), in blood samples of experimental rats (30), and in the liver and spleen of a horse with fatal idiopathic hepatopathy (31). These discoveries suggest that gemycircularviruses represent a group of emerging viruses, which are widespread in nature, and some of them may impact human and animal health.…”

mentioning

confidence: 99%

Fungal DNA virus infects a mycophagous insect and utilizes it as a transmission vector

Liu

Xiè

Chéng

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

147

View full text Add to dashboard Cite

Mycoviruses are usually transmitted horizontally via hyphal anastomosis and vertically via sexual/asexual spores. Previously, we reported that a gemycircularvirus, Sclerotinia sclerotiorum hypovirulenceassociated DNA virus 1 (SsHADV-1), could infect its fungal host extracellularly. Here, we discovered that SsHADV-1 could infect a mycophagous insect, Lycoriella ingenua, and use it as a transmission vector. Virus acquired by larvae feeding on colonies of a virus-infected strain of S. sclerotiorum was replicated and retained in larvae, pupae, adults, and eggs. Virus could be transmitted to insect offspring when larvae were injected with virus particles and allowed to feed on a nonhost fungus. Virus replication in insect cells was further confirmed by inoculating Spodoptera frugiperda cells with virus particles and analyzing with RT-PCR, Northern blot, immunofluorescence, and flow cytometry assays. Larvae could transmit virus once they acquired virus by feeding on virus-infected fungal colony. Offspring larvae hatched from viruliferous eggs were virus carriers and could also successfully transmit virus. Virus transmission between insect and fungus also occurred on rapeseed plants. Virus-infected isolates produced less repellent volatile substances to attract adults of L. ingenua. Furthermore, L. ingenua was easily observed on Sclerotinia lesions in rapeseed fields, and viruliferous adults were captured from fields either sprayed with a virus-infected fungal strain or nonsprayed. Our findings may facilitate the exploration of mycoviruses for control of fungal diseases and enhance our understanding of the ecology of SsHADV-1 and other newly emerging SsHADV-1-like viruses, which were recently found to be widespread in various niches including human HIV-infected blood, human and animal feces, insects, plants, and even sewage. mycovirus | biological control | mycophagous insect | mutualism | gemycircularvirus

show abstract

Divergent Gemycircularvirus in HIV-Positive Blood, France

Cited by 29 publications

References 10 publications

Viral nucleic acids in human plasma pools

Viral nucleic acids in human plasma pools

Human pegivirus isolates characterized by deep sequencing from hepatitis C virus‐RNA and human immunodeficiency virus‐RNA–positive blood donations, France

Fungal DNA virus infects a mycophagous insect and utilizes it as a transmission vector

Contact Info

Product

Resources

About