Divergent effect of glucagon antibodies on arginine and glucose-stimulated insulin secretion in the rat

Tan, K.S.; Atabani, G.

doi:10.1007/bf00280888

Cited by 14 publications

(2 citation statements)

References 16 publications

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“…Arginine is a potent stimulator of insulin secretion both in vivo (Fajans et al, 1967;Efendic et al, 1971) and in vitro (Milner, 1970). Recent work, using anti-glucagon antibodies to control for the effect of this hormone (Tan, Atabani and Marks, 1985), suggests that arginine stimulates both glucagon and insulin secretion in a step-wise manner, with glucagon acting as a mediator or potentiator of the insulinotropic effect. The hyperglycaemic effect of glucagon may explain the transient rise in mean blood glucose after arginine seen in our experiments.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of Portal Versus Systemic Venous Drainage in Murine Foetal Pancreatic Islet Transplantation

Mandel

1986

Aust J Exp Biol Med

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Summary.We have compared the efficiency of foetal islet graft growth and function in renal subcapsular (systemic drainage) and splenic (portal drainage) sites. Age-matched female BALB/c streptozotocin (STZ)-diabetic mice were grafted either under the left renal capsule or on to the hilar surface of the spleen with one half of a syngeneic 17-day gestation foetal -pancreas which had been in organ culture for 2 weeks. Gain in body weight, return to jiormoglycaemia and to normal glycosylated haemoglobin levels were not significantly different between the two groups. However, when an intraperitoneal arginine challenge was performed the portally grafted mice showed more normal responses than the systemically grafted mice. Although still not normal after the arginine challenge, mean blood glucose values were lower and serum insulin values higher in the spleen grafted animals than in the renal subcapsular graft group. The total amount of insulin was assayed by extraction of both the graft and the pancreas of each animal. The amount of insulin in the grafts from the spleen was not significantly different from those in the renal subcapsular site. We conclude that the efficiency of a graft draining into the portal circulation is greater than an equivalent sized graft draining systemically when a maxima! challenge is applied.

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Section: Discussionmentioning

confidence: 99%

A Comparison of Portal Versus Systemic Venous Drainage in Murine Foetal Pancreatic Islet Transplantation

Mandel

1986

Aust J Exp Biol Med

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“…Six mol/g of histidine lowered plasma insulin levels to 0.8 + 0.1 ng/ml. In mammals, the stimulatory action of arginine on insulin secretion is mediated through glucagon (Tan et al 1985). Therefore, acute glucagon insufficiency, caused by anti-GLU sera, eliminates the arginine effect on insulin.…”

Section: Effects Of Pancreatic Hormones On Fish Growth and Metabolismmentioning

confidence: 99%

Physiology of fish endocrine pancreas

Plisetskaya

1989

Fish Physiol Biochem

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From the very beginning of physiological studies on the endocine pancreas, fish have been used as experimental subjects. Fish insulin was one of the first vertebrate insulins isolated and one of the first insulins whose primary and then tertiary structures were reported. Before a second pancreatic hormone, glucagon, was characterized, a physiologically active 'impurity', similar to that in mammalian insulin preparations, was found in fish insulins.Fish have become the most widely used model for studies of biosynthesis and processing of the pancreatic hormones. It seems inconceivable, therefore, that until the recent past cod and tuna insulins have been the only purified piscine islet hormones available for physiological experiments. The situation has changed remarkably during the last decade.In this review the contemporary status of physiological studies on the fish pancreas is outlined with an emphasis on the following topics: 1) contents of pancreatic peptides in plasma and in islet tissue; 2) actions of piscine pancreatic hormones in fish; 3) specific metabolic consequences of an acute insufficiency of pancreatic peptides; 4) functional interrelations among pancreatic peptides which differ from those of mammals. The pitfalls, lacunae and the perspectives of contemporary physiological studies on fish endocrine pancreas are outlined.

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Metabolic changes in coho and chinook salmon resulting from acute insufficiency in pancreatic hormones

1989

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Acute deficiency in pancreatic peptides (insulin, somatostatin-25, glucagon, and glucagon-like peptide) was invoked for 9-12 hr in coho, Oncorhynchus kisutch, and chinook, O. tshawytscha, salmon by administration of specific antisera raised against purified salmon hormones. Insulin-deficient fish were hyperglycemic, had diminished glycogen content in the liver (Plisetskaya et al., '88a, elevated liver triacylglycerol lipase activity, and higher concentration of plasma triiodothyronine (T3) compared to a control group of fish injected with nonspecific rabbit serum. After immunoneutralization of somatostatin-25, fish remained normoglycemic, with higher liver glycogen content, decreased lipase activity, and elevated plasma levels of insulin, while the levels of T3 declined. The induced deficiency in glucagon family peptides led to comparatively smaller changes: liver glycogen content was increased after anti-glucagon-like peptide (aGLP) injection and transient hyperglycemia was apparent following anti-glucagon (aGLU) administration. Circulating levels of insulin remained unaffected for at least 9 hr following aGLU and aGLP treatments. The velocity of pyruvate kinase at 2.5 mM phosphoenolpyruvate (V2.5) was depressed, especially after the combined administration of aGLU + aGLP. The effectiveness of immunoneutralization experiments was greatly dependent on the particular stage of the fish life cycle. Antisera against fish pancreatic peptides proved to be a suitable tool in the studies of hormonal regulation of fish metabolism.

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Divergent effect of glucagon antibodies on arginine and glucose-stimulated insulin secretion in the rat

Cited by 14 publications

References 16 publications

A Comparison of Portal Versus Systemic Venous Drainage in Murine Foetal Pancreatic Islet Transplantation

A Comparison of Portal Versus Systemic Venous Drainage in Murine Foetal Pancreatic Islet Transplantation

Physiology of fish endocrine pancreas

Metabolic changes in coho and chinook salmon resulting from acute insufficiency in pancreatic hormones

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