Divergent biochemical and enzymatic properties of oxalate oxidase isoforms encoded by four similar genes in rice

Li, Xiao Chun; Liao, Yuan Yang; Leung, David W. M.; Wang, Hai Yan; Chen, Bai Ling; Peng, Xin; Liu, E.E.

doi:10.1016/j.phytochem.2015.08.019

Cited by 11 publications

(7 citation statements)

References 41 publications

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“…OsOXO4 is the only gene whose transcriptional response to M. oryzae is amplified by high nitrogen. However, the role of OsOX4 in rice blast resistance is not clear (Zhang et al, 2013 ; Li et al, 2015 ). Finally, we identified in our datasets 11 genes coding for enzymes potentially involved in the TCA cycle replenishment or GABA shunt, 9 of which are not differentially expressed.…”

Section: Resultsmentioning

confidence: 99%

Increase of Fungal Pathogenicity and Role of Plant Glutamine in Nitrogen-Induced Susceptibility (NIS) To Rice Blast

Huang

Thu

et al. 2017

Front. Plant Sci.

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Highlight Modifications in glutamine synthetase OsGS1-2 expression and fungal pathogenicity underlie nitrogen-induced susceptibility to rice blast.Understanding why nitrogen fertilization increase the impact of many plant diseases is of major importance. The interaction between Magnaporthe oryzae and rice was used as a model for analyzing the molecular mechanisms underlying Nitrogen-Induced Susceptibility (NIS). We show that our experimental system in which nitrogen supply strongly affects rice blast susceptibility only slightly affects plant growth. In order to get insights into the mechanisms of NIS, we conducted a dual RNA-seq experiment on rice infected tissues under two nitrogen fertilization regimes. On the one hand, we show that enhanced susceptibility was visible despite an over-induction of defense gene expression by infection under high nitrogen regime. On the other hand, the fungus expressed to high levels effectors and pathogenicity-related genes in plants under high nitrogen regime. We propose that in plants supplied with elevated nitrogen fertilization, the observed enhanced induction of plant defense is over-passed by an increase in the expression of the fungal pathogenicity program, thus leading to enhanced susceptibility. Moreover, some rice genes implicated in nitrogen recycling were highly induced during NIS. We further demonstrate that the OsGS1-2 glutamine synthetase gene enhances plant resistance to M. oryzae and abolishes NIS and pinpoint glutamine as a potential key nutrient during NIS.

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Section: Resultsmentioning

confidence: 99%

Increase of Fungal Pathogenicity and Role of Plant Glutamine in Nitrogen-Induced Susceptibility (NIS) To Rice Blast

Huang

Thu

et al. 2017

Front. Plant Sci.

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“…To the best of our knowledge, however, such a glyoxylate dehydrogenase that can reversibly catalyse the inter-conversion between oxalyl-CoA and glyoxylate has not been identified so far in plants. In addition, oxalate can also be directly oxidised into CO 2 through the catalysis of oxalate oxidase, but this reaction seems less important for oxalate catabolism in plants, since the majority of plant species contain no such enzyme (Zhang et al 2013;Li et al 2015). The location of OCS in the oxalate metabolism network is illustrated in Fig.…”

Section: Discussionmentioning

confidence: 99%

The oxalyl‐CoA synthetase‐regulated oxalate and its distinct effects on resistance to bacterial blight and aluminium toxicity in rice

et al. 2017

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ABSTRACT• Oxalic acid is widely distributed in biological systems and known to play functional roles in plants. The gene AAE3 was recently identified to encode an oxalyl-CoA synthetase (OCS) in Arabidopsis that catalyses the conversion of oxalate and CoA into oxalyl-CoA. It will be particularly important to characterise the homologous gene in rice since rice is not only a monocotyledonous model plant, but also a staple food crop.• Various enzymatic and biological methods have been used to characterise the homologous gene.• We first defined that AAE3 in the rice genome (OsAAE3) . Chemical modification and site-directed mutagenesis analyses identified thiols as the active site residues for rice OCS catalysis, suggesting that the enzyme might be regulated by redox state. Subcellular localisation assay showed that the enzyme is located in the cytosol and predominantly distributed in leaf epidermal cells. As expected, oxalate levels increased when OCS was suppressed in RNAi transgenic plants. More interestingly, OCS-suppressed plants were more susceptible to bacterial blight but more resistant to Al toxicity.• The results demonstrate that the OsAAE3-encoded protein also acts as an OCS in rice, and may play different roles in coping with stresses. These molecular, enzymatic and functional data provide first-hand information to further clarify the function and mechanism of OCS in rice plants.

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“…As illustrated in Figure 1A, in this bypass glycolate is first oxidized to oxalate in two steps by OsGLO3, an isoform of glycolate oxidase in rice that was shown to effectively catalyze the oxidation of both glycolate and glyoxylate (Zhang et al, 2012). The oxalate produced is then completely oxidized into CO 2 and H 2 O 2 by OsOXO3, an isoform of oxalate oxidase in rice that has a stable optimum pH ranging from 6.0 to 8.0 (Li et al, 2015). H 2 O 2 produced by the three reactions is scavenged by OsCATC, an isoform of catalase in rice leaves (Zhang et al, 2016).…”

Section: Establishment Of a New Photorespiratory Bypass In Rice Chlormentioning

confidence: 99%

Engineering a New Chloroplastic Photorespiratory Bypass to Increase Photosynthetic Efficiency and Productivity in Rice

et al. 2019

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Over the past few years, three photorespiratory bypasses have been introduced into plants, two of which led to observable increases in photosynthesis and biomass yield. However, most of the experiments were carried out using Arabidopsis under controlled environmental conditions, and the increases were only observed under low-light and short-day conditions. In this study, we designed a new photorespiratory bypass (called GOC bypass), characterized by no reducing equivalents being produced during a complete oxidation of glycolate into CO 2 catalyzed by three rice-self-originating enzymes, i.e., glycolate oxidase, oxalate oxidase, and catalase. We successfully established this bypass in rice chloroplasts using a multi-gene assembly and transformation system. Transgenic rice plants carrying GOC bypass (GOC plants) showed significant increases in photosynthesis efficiency, biomass yield, and nitrogen content, as well as several other CO 2 -enriched phenotypes under both greenhouse and field conditions. Grain yield of GOC plants varied depending on seeding season and was increased significantly in the spring. We further demonstrated that GOC plants had significant advantages under high-light conditions and that the improvements in GOC plants resulted primarily from a photosynthetic CO 2 -concentrating effect rather than from improved energy balance. Taken together, our results reveal that engineering a newly designed chloroplastic photorespiratory bypass could increase photosynthetic efficiency and yield of rice plants grown in field conditions, particularly under high light.

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Divergent biochemical and enzymatic properties of oxalate oxidase isoforms encoded by four similar genes in rice

Cited by 11 publications

References 41 publications

Increase of Fungal Pathogenicity and Role of Plant Glutamine in Nitrogen-Induced Susceptibility (NIS) To Rice Blast

Increase of Fungal Pathogenicity and Role of Plant Glutamine in Nitrogen-Induced Susceptibility (NIS) To Rice Blast

The oxalyl‐CoA synthetase‐regulated oxalate and its distinct effects on resistance to bacterial blight and aluminium toxicity in rice

Engineering a New Chloroplastic Photorespiratory Bypass to Increase Photosynthetic Efficiency and Productivity in Rice

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