Divergence of reactions to arsenic (As) toxicity in tobacco (Nicotiana benthamiana) plants: A lesson from peroxidase involvement

Lukačová, Zuzana; Bokor, Boris; Vávrová, Silvia; Šoltýs, Katarína; Vaculík, Marek

doi:10.1016/j.jhazmat.2021.126049

Cited by 12 publications

(13 citation statements)

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“…Maintaining cell homeostasis during a stress reaction is a key in enabling plants to survive any sub-optimal conditions. To keep ROS content at the nontoxic level, the involvement of antioxidant enzymes is essential; however, stimulation of their activity is not always obvious [28,32,56,57]. A common phenomenon of significantly higher POX activity in the roots than in the leaves was also confirmed in the present study (Figure 7).…”

Section: Discussionsupporting

confidence: 82%

“…In the present study, As stress reaction was evaluated. Basic growth characteristics were negatively changed by As itself, which is a common phenomenon of this toxic metalloid [28,55] (Figures 5 and 6, Table 1). Plants of the priming PAW As treatment accumulated more water than As-treated plants (Figure 5a), but no improvement of the FW was detected in the rolls PAW treatment.…”

Section: Discussionmentioning

confidence: 99%

“…The production of antioxidants in the plants challenged by As is a common phenomenon [58]. However, a divergent reaction of POX activity and pattern in POX expression was documented, caused by different As doses in tobacco (Nicotiana benthamiana) plants [28]. It is clear that plants reacted with elevated POX activities on moderate As stress, but the reaction was different for low and high As doses.…”

Section: Discussionmentioning

confidence: 99%

“…Among them, one of the most dangerous is metalloid arsenic (As), absorbed and translocated in the plant bodies into the edible parts where it threatens the highest trophic levels [26]. A common plant reaction to As stress is an overproduction of various reactive oxygen species (ROS) within the plant body [27] triggering antioxidant systems [28]. H 2 O 2 plays a central role in stress signal transduction [27,29]; a delicate balance between its production and scavenging must be maintained in the plant cells.…”

Section: Introductionmentioning

confidence: 99%

“…Systems joined with ROS in terms of their metabolism are either enzymatic or non-enzymatic. The most active enzymatic scavengers of H 2 O 2 are catalase (CAT), decomposing it directly, and peroxidases (POX), reducing it by oxidizing various substrate, e.g., monolignols in the cell wall, which is an important step in lignin formation [28,[31][32][33]. A promising method of inducing stress resistance in plants is the pre-treatment (priming) of seeds or plants by exposure to a chemical compound acting as a stressor [27,[34][35][36].…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Plasma Activated Water on Maize (Zea mays L.) under Arsenic Stress

Lukačová

Švubová

Selvekova

et al. 2021

Plants

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Plasma activated water (PAW) is a source of various chemical species useful for plant growth, development, and stress response. In the present study, PAW was generated by a transient spark discharge (TS) operated in ambient air and used on maize corns and seedlings in the 3 day paper rolls cultivation followed by 10 day hydroponics cultivation. For 3 day cultivation, two pre-treatments were established, “priming PAW” and “rolls PAW”, with corns imbibed for 6 h in the PAW and then watered daily by fresh water and PAW, respectively. The roots and the shoot were then analyzed for guaiacol peroxidase (G-POX, POX) activity, root tissues for their lignification, and root cell walls for in situ POX activity. To evaluate the potential of PAW in the alleviation abiotic stress, ten randomly selected seedlings were hydroponically cultivated for the following 10 days in 0.5 Hoagland nutrient solutions with and without 150 μM As. The seedlings were then analyzed for POX and catalase (CAT) activities after As treatment, their leaves for photosynthetic pigments concentration, and leaves and roots for As concentration. The PAW improved the growth of the 3 day-old seedlings in terms of the root and the shoot length, while roots revealed accelerated endodermal development. After the following 10 day cultivation, roots from PAW pre-treatment were shorter and thinner but more branched than the control roots. The PAW also enhanced the POX activity immediately after the imbibition and in the 3 day old roots. After 10 day hydroponic cultivation, antioxidant response depended on the PAW pre-treatment. CAT activity was higher in As treatments compared to the corresponding PAW treatments, while POX activity was not obvious, and its elevated activity was found only in the priming PAW treatment. The PAW pre-treatment protected chlorophylls in the following treatments combined with As, while carotenoids increased in treatments despite PAW pre-treatment. Finally, the accumulation of As in the roots was not affected by PAW pre-treatment but increased in the leaves.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%