Divergence and conservation of defensins and lipid transfer proteins (LTPs) from sugarcane wild species and modern cultivar genomes

Silva, Leandro de Oliveira; Pereira, Lídia da Silva; Pereira, Jacymara Lopes; Gomes, Valdirene Moreira; Grativol, Clícia

doi:10.1007/s10142-022-00832-0

Cited by 4 publications

(4 citation statements)

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“…In this study, we identified 71 nsLTP genes with 66 alleles in S. spontaneum AP85-441; Type I had largest number of nsLTP genes (22). Recently, de Oliveira Silva et al (2022) used 16 sequences of Type I LTPs from sorghum ( S. bicolor L. Moench) ( Wang et al, 2012 ) as bait to search for nsLTP members in S. spontaneum and identified 21 gene members of nsLTP Type I. High variability of nsLTP family members among different plant species might be due to different polyploidization or the duplication of entire genomes during plant evolution ( Van de Peer et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…Overexpression of NtLTPI.4/III.11 in tobacco enhanced tolerance to Ralstonia solanacearum ( Yang et al, 2022 ). Notably, expression of ShnsLTPI.8 ( Sspon.07G0018180-2B ) and ShnsLTPI.10 ( Sspon.07G0018180-3C ) was upregulated in sugarcane infected with A. avenae ( de Oliveira Silva et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…vasinfectum ) in transgenic Arabidopsis and silenced cotton by modulating phenylpropanoid homeostasis and/or ROS accumulation ( Chen et al, 2021 ). Recently, de Oliveira Silva et al (2022) demonstrated that SsnsLtpI.3 ( Sspon.03G0003670-4D ) and SsnsLtpV.4 ( Sspon.05G0025940-2C ) play a negative regulatory role in response of sugarcane to A. avenae infection. Overall, functional redundancy and divergence within one gene family in plants, particularly sugarcane, which is a complex polyploidy crop, is frequent.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a nsLTP (Cluster-4871.183445) from sugarcane was shown to act as a potentially positive regulator of the defense response to Xanthomonas albilineans ( Xa ), the bacterial pathogen of leaf scald ( Meng et al, 2020 ). Furthermore, numerous nsLTP genes were identified in sugarcane in response to infection with beneficial bacteria ( Gluconacetobacter diazotrophicus ) and Acidovorax avenae , the causative bacterial pathogen for red stripe disease, as well as water depletion ( de Oliveira Silva et al, 2022 ). Apart from these observations, systematic identification and expression analysis of the nsLTP gene family in sugarcane under diverse environmental stress is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Systematic and functional analysis of non-specific lipid transfer protein family genes in sugarcane under Xanthomonas albilineans infection and salicylic acid treatment

Zhao²,

Shi³

et al. 2022

Front. Plant Sci.

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Plant non-specific lipid transfer proteins (nsLTPs) are small basic proteins that play a significant regulatory role in a wide range of physiological processes. To date, no genome-wide survey and expression analysis of this gene family in sugarcane has been performed. In this study we identified the nsLTP gene family in Saccharum spontaneum and carried out expression profiling of nsLTPs in two sugarcane cultivars (Saccharum spp.) that have different resistance to leaf scald caused by Xanthomonas albilineans (Xa) infection. The effect of stress related to exogenous salicylic acid (SA) treatment was also examined. At a genome-wide level, S. spontaneum AP85-441 had 71 SsnsLTP genes including 66 alleles. Tandem (9 gene pairs) and segmental (36 gene pairs) duplication events contributed to SsnsLTP gene family expansion. Five SsnsLTP proteins were predicted to interact with five other proteins. Expression of ShnsLTPI.8/10/Gb.1 genes was significantly upregulated in LCP85-384 (resistant cultivar), but downregulated in ROC20 (susceptible cultivar), suggesting that these genes play a positive regulatory role in response of sugarcane to Xa infection. Conversely, ShnsLTPGa.4/Ge.3 appears to act as a negative regulator in response Xa infection. The majority (16/17) of tested genes were positively induced in LCP85-384 72 h after SA treatment. In both cultivars, but particularly in LCP85-384, ShnsLTPIV.3/VIII.1 genes were upregulated at all time-points, suggesting that the two genes might act as positive regulators under SA stress. Meanwhile, both cultivars showed downregulated ShnsLTPGb.1 gene expression, indicating its potential negative role in SA treatment responses. Notably, the ShnsLTPGb.1 gene had contrasting effects, with positive regulation of gene expression in response to Xa infection and negative regulation induced by SA stress. Together, our results provide valuable information for elucidating the function of ShnsLTP family members under two stressors and identified novel gene sources for development of sugarcane that are tolerant of environmental stimuli.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%