Diurnal variation in CREB phosphorylation and PER1 protein levels in lactotroph cells of melatonin-proficient C3H and melatonin-deficient C57BL mice: similarities and differences

Sheynzon, Pjotr; Karolczak, Magdalena; Dehghani, Faramarz; Korf, Horst‐Werner

doi:10.1007/s00441-005-1150-4

Cited by 6 publications

(4 citation statements)

References 47 publications

(45 reference statements)

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“…Although it is unclear which pituitary cells are responsible for the PER2Luc rhythm, clock genes are expressed in corticotrophs, the source of ACTH (9, 45) and lactotrophs, the source of prolactin (39). Since both corticotrophs and lactotrophs are stress-responsive cells, the phase advance in the PER2Luc rhythm might reflect changes in clock rhythms associated with these cell types.…”

Section: Discussionmentioning

confidence: 98%

Chronic subordination stress phase advances adrenal and anterior pituitary clock gene rhythms

Razzoli¹,

Karsten

Yoder

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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Circadian rhythms in glucocorticoids are the product of interactions between the hypothalamic-pituitary-adrenal (HPA) axis and the mammalian clock gene system. The adrenal clock can generate the glucocorticoid rhythm that in turn synchronizes other peripheral clocks to maintain homeostasis. Stress acutely activates and chronically upregulates the HPA axis, suggesting that the adrenal clock could be modulated by stress. However, there is no direct evidence that stress affects the adrenal clock rhythm. We tested the hypothesis that a model of chronic subordination stress (CSS) that has a major impact on HPA axis regulation, metabolism, and emotional behavior alters adrenal and pituitary clock gene rhythms. Clock gene rhythms were assessed using mPER2::Luciferase (PER2Luc) knockin mice in which in vitro bioluminescence rhythms reflect the Per2 clock gene expression. PER2Luc mice that experienced CSS for 2 wk showed positive energy balance reflected by increased body weight and food intake. Additionally, CSS phase advanced the adrenal (∼2 h) and the pituitary (∼1 h) PER2Luc rhythm compared with control mice. The activity rhythm was not affected. The adrenal clock phase shift was associated with increased feed conversion efficiency, suggesting that the metabolic phenotype in CSS mice may be related to altered adrenal clock rhythmicity. Interestingly, a single subordination experience followed by 8 h sensory housing also phase advanced the adrenal, but not the pituitary, PER2Luc rhythm. Overall, these data demonstrate a stress-induced phase shift in a peripheral clock gene rhythm and differential stress sensitivity of two peripheral clocks within the HPA axis, suggesting a link between clock desynchrony and individual vulnerability to stress.

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Section: Discussionmentioning

confidence: 98%

Chronic subordination stress phase advances adrenal and anterior pituitary clock gene rhythms

Razzoli¹,

Karsten

Yoder

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Studies of other groups investigating the impact of melatonin on CREB phosphorylation were initially concentrated on neuronal tissue, for example, the suprachiasmatic nucleus [25–27], the pineal organ [28, 29] and the pars tuberalis [30]; in these tissues, CREB phosphorylation plays an important role in the rhythmic activation and synchronisation of gene expression [31]. McNulty and colleagues [32] were the first authors to show that melatonin is capable of inhibiting, in a dose‐dependent manner, the forskolin‐induced phosphorylation of CREB within primary cell cultures of the pars tuberalis.…”

Section: Introductionmentioning

confidence: 99%

Phosphorylation of cyclic AMP‐response element–binding protein (CREB) is influenced by melatonin treatment in pancreatic rat insulinoma β‐cells (INS‐1)

Bazwinsky-Wutschke

Wolgast

Mühlbauer

et al. 2012

Journal of Pineal Research

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The pineal hormone melatonin exerts its influence on the insulin secretion of pancreatic islets by a variety of signalling pathways. The purpose of the present study was to analyse the impact of melatonin on the phosphorylated transcription factor cAMP-response element-binding protein (pCREB). In pancreatic rat insulinoma β-cells (INS-1), pCREB immunofluorescence intensities in cell nuclei using digitised confocal image analysis were measured to semi-quantify differences in the pCREB immunoreactivity (pCREB-ir) caused by different treatments. Increasing concentrations of forskolin or 3-isobutyl-1-methylxanthine (IBMX) resulted in a dose-dependent rise of the mean fluorescence intensity in pCREB-ir nuclear staining. Concomitant melatonin application significantly decreased pCREB-ir in INS-1 cells after 30-min, 1-hr and 3-hr treatment. The melatonin receptor antagonists luzindole and 4-phenyl-2-propionamidotetraline (4P-PDOT) completely abolished the pCREB phosphorylation-decreasing effect of melatonin, indicating that both melatonin receptor isoforms (MT(1) and MT(2)) are involved. In a transfected INS-1 cell line expressing the human MT(2) receptor, melatonin caused the greatest reduction in pCREB after IBMX treatment compared with nontransfected INS-1 cells, indicating a crucial influence of melatonin receptor density on pCREB regulation. Furthermore, the downregulation of pCREB by melatonin is concomitantly associated with a statistically significant downregulation of Camk2d transcript levels, as measured after 3 hr. In conclusion, the present study provides evidence that the phosphorylation level of CREB is modulated in pancreatic β-cells by melatonin. Mediated via CREB, melatonin regulates the expression of genes that play an important functional role in the regulation of β-cell signalling pathways.

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“…Although we did not measure melatonin in our group of animals, it has been well established that melatonin proficiency is an existing difference between the two strains (Kasahara et al, 2010; Kennaway, 2019; Roseboom et al, 1998; von Gall et al, 2000), and previous articles comparing C57 and C3H mice have discussed the observed differences in this context (Dinet et al, 2007; Homola et al, 2015; Metzger et al, 2020; Pfeffer et al, 2017; Sheynzon et al, 2005; Stehle et al, 2002). Since melatonin is known to be involved in sleep regulation we think it is appropriate to discuss its putative influence shortly.…”

Section: Discussionmentioning

confidence: 99%

“…Comparisons between C57 and C3H mice have been performed previously to approximate the role and effects of melatonin on physiology and behaviour. These comparisons show, for example, differences in retinal clock gene expression (Dinet et al, 2007), spontaneous cage behaviour (Adamah‐Biassi et al, 2013), and seasonal behaviour (Metzger et al, 2020) among many more (Brednow & Korf, 1998; Homola et al, 2015; Pfeffer et al, 2017; Sheynzon et al, 2005; Veasey et al, 2000; von Gall et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Sleep and the sleep electroencephalogram in C57BL/6 and C3H/HeN mice

van Dorp,

Rolleri,

Deboer

2023

Journal of Sleep Research

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SummaryDifferent mouse strains used in biomedical research show different phenotypes associated with their genotypes. Two mouse strains commonly used in biomedical sleep research are C57Bl/6 and C3H/He, the strains differ in numerous aspects, including their ability to secrete melatonin as well as the expression of several sleep‐related genes. However, sleep regulation has only limitedly been compared between C3H/HeN and C57Bl/6 mice. We therefore compared sleep–wake behaviour and EEG‐measured spectral brain activity for C57bl/6 and C3H/HeN mice during a 12:12 h light: dark baseline and during and after a 6 h sleep deprivation. The C3H mice spent more time in NREM sleep around the light–dark transition and more time in REM sleep during the dark phase compared with C57bl/6 mice. The C3H mice also showed more EEG activity in the 4.5–7.5 Hz range during all stages and a stronger 24 h modulation of EEG power density in almost all EEG frequencies during NREM sleep. After the sleep deprivation, C3H mice showed a stronger recovery response, which was expressed in both a larger increase in EEG slow wave activity (SWA) and more time spent in NREM sleep. We show large differences regarding sleep architecture and EEG activity between C3H and C57bl/6 mice. These differences include the amount of waking during the late dark phase, the 24 h amplitude in EEG power density, and the amount of REM sleep during the dark phase. We conclude that differences between mouse strains should be considered when selecting a model strain to improve the generalisability of studies investigating biomedical parameters related to sleep and circadian rhythms.

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Diurnal variation in CREB phosphorylation and PER1 protein levels in lactotroph cells of melatonin-proficient C3H and melatonin-deficient C57BL mice: similarities and differences

Cited by 6 publications

References 47 publications

Chronic subordination stress phase advances adrenal and anterior pituitary clock gene rhythms

Chronic subordination stress phase advances adrenal and anterior pituitary clock gene rhythms

Phosphorylation of cyclic AMP‐response element–binding protein (CREB) is influenced by melatonin treatment in pancreatic rat insulinoma β‐cells (INS‐1)

Sleep and the sleep electroencephalogram in C57BL/6 and C3H/HeN mice

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