Diuretic hormone 44 receptor in Malpighian tubules of the mosquito <i>Aedes aegypti</i>: evidence for transcriptional regulation paralleling urination

Jagge, Christopher; Pietrantonio, Patricia V.

doi:10.1111/j.1365-2583.2008.00817.x

Cited by 31 publications

(18 citation statements)

References 97 publications

(146 reference statements)

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“…As expected, the transcriptome of the Malpighian tubules alters significantly between these two opposing states, particularly for a subset of diuretic peptide receptors, ion transporters and water channels (Table 3). Receptors for the diuretic peptides Anoga-DH 31 and Anoga-DH 44 have been identified in the Anopheles tubules; GPRDIH1 is the putative receptor for Anoga-DH 44 (Jagge and Pietrantonio, 2008), and GPRCAL1 for Anoga-DH 31 (Coast et al, 2005). Our data show that in the larval tubules GPRDIH1 is strongly expressed, with very low GPRCAL1 expression, and in adults the opposite -GPRCAL1 is strongly expressed, with lower GPRDIH1 (Table 3).…”

Section: Cell Signalling In Larval Versus Adult Tubulesmentioning

confidence: 58%

A comprehensive transcriptomic view of renal function in the malaria vector, Anopheles gambiae

Overend

Cabrero

Halberg

et al. 2015

Insect Biochemistry and Molecular Biology

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Renal function is essential to maintain homeostasis. This is particularly significant for insects that undergo complete metamorphosis; larval mosquitoes must survive a freshwater habitat whereas adults are terrestrial, and mature females must maintain ion and fluid homeostasis after blood feeding. To investigate the physiological adaptations required for successful development to adulthood, we studied the Malpighian tubule transcriptome of Anopheles gambiae using Affymetrix arrays. We assessed transcription under several conditions; as third instar larvae, as adult males fed on sugar, as adult females fed on sugar, and adult females after a blood meal. In addition to providing the most detailed transcriptomic data to date on the Anopheles Malpighian tubules, the data provide unique information on the renal adaptations required for the switch from freshwater to terrestrial habitats, on gender differences, and on the contrast between nectar-feeding and haematophagy. We found clear differences associated with ontogenetic change in lifestyle, gender and diet, particularly in the neuropeptide receptors that control fluid secretion, and the water and ion transporters that impact volume and composition. These data were also combined with transcriptomics from the Drosophila melanogaster tubule, allowing meta-analysis of the genes which underpin tubule function across Diptera. To further investigate renal conservation across species we selected four Drosophila melanogaster genes with orthologues highly enriched in the Anopheles tubules, and generated RNAi knockdown flies. Three of these genes proved essential, showing conservation of critical functions across 150 million years of phylogenetic separation. This extensive data-set is available as an online resource, MozTubules.org, and could potentially be mined for novel insecticide targets that can impact this critical organ in this pest species.

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Section: Cell Signalling In Larval Versus Adult Tubulesmentioning

confidence: 58%

A comprehensive transcriptomic view of renal function in the malaria vector, Anopheles gambiae

Overend

Cabrero

Halberg

et al. 2015

Insect Biochemistry and Molecular Biology

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“…The potential to generate null alleles for CG12370 in Drosophila will offer further insight into the roles of this receptor molecule in refining the specific aspects of DH44-R2 signaling pertaining to osmoregulation and osmotic homeostasis. Of interest, expression of the mosquito CG12370 homolog is altered during times of increased urination (Jagge and Pietrantonio, 2008), suggesting that the expression of DH44-R2 may be linked to the hormonal modulation of tubule physiology. By contrast, DH44-R1 appears to be limited in its expression in the brain, and is specifically expressed in neurons that express the corazonin neuropeptide .…”

Section: Discussionmentioning

confidence: 99%

Functional differences between two CRF-related diuretic hormone receptors in Drosophila

Hector

Bretz

Zhao

et al. 2009

Journal of Experimental Biology

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SUMMARYIn Drosophila, two related G-protein-coupled receptors are members of the corticotropin releasing factor (CRF) receptor subfamily. We have previously reported that one of these receptors, encoded by CG8422 is a functional receptor for a diuretic hormone, DH 44 . Here, we report that the other CRF receptor subfamily member, encoded by CG12370, is also a receptor for the DH 44 neuropeptide. The lines of evidence to support this identification include increases in cAMP levels due to CG12370 receptor activation and the recruitment of β-arrestin-GFP to the plasma membrane in response to DH 44 application. We compared these features of the receptors DH44-R2 (encoded by CG12370) and DH44-R1 (encoded by CG8422) and found fundamental differences in signaling, association with the arrestins, and peptide sensitivity. We found that the sensitivity of DH44-R2 to the DH 44 peptide is lower than that of DH44-R1, specifically an estimated EC 50 of 7.98E-07 mol l -1 for DH 44 by DH44-R2 to an EC 50 of 5.12E-09 mol l -1 by DH44-R1 and found that previous reports on the sensitivity of the tubule to DH 44 is in agreement with our measurements of DH44-R2 sensitivity. We employed a specific RNAi construct to selectively knock-down DH44-R2 expression and this led to heightened sensitivity to osmotic challenges. The functional characterization of this diuretic hormone receptor in Drosophila demonstrates a high degree of conservation of CRF-like signaling.

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“…Within the first hour after taking a blood meal (post blood meal - PBM) mosquito females can discharge more than 40% of water and sodium enclosed in the blood plasma [1]. Diuresis is under hormonal control by neuropeptide hormones secreted by the central nervous system [2]. In the current model, diuretic hormones, released seconds after start of the blood meal, stimulate the MT cells to produce the second messenger molecule cAMP which activates transcellular diuresis by increasing transepithelial cation (Na + , K + ) transport [2].…”

Section: Introductionmentioning

confidence: 99%

“…Diuresis is under hormonal control by neuropeptide hormones secreted by the central nervous system [2]. In the current model, diuretic hormones, released seconds after start of the blood meal, stimulate the MT cells to produce the second messenger molecule cAMP which activates transcellular diuresis by increasing transepithelial cation (Na + , K + ) transport [2]. Another class of neuropeptide hormones, the kinins, increase intracellular calcium levels that regulate anion movement (Cl − ) into the MT lumen [3], [4].…”

Section: Introductionmentioning

confidence: 99%

The Aquaporin Gene Family of the Yellow Fever Mosquito, Aedes aegypti

et al. 2010

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BackgroundThe mosquito, Aedes aegypti, is the principal vector of the Dengue and yellow fever viruses. During feeding, an adult female can take up more than its own body weight in vertebrate blood. After a blood meal females excrete large amounts of urine through their excretion system, the Malpighian tubules (MT). Diuresis starts within seconds after the mosquito starts feeding. Aquaporins (AQPs) are a family of membrane transporters that regulate the flow of water, glycerol and other small molecules across cellular membranes in both prokaryotic and eukaryotic cells. Our aim was to identify aquaporins that function as water channels, mediating transcellular water transport in MTs of adult female Ae. aegypti.Methodology/Principal FindingsUsing a bioinformatics approach we screened genome databases and identified six putative AQPs in the genome of Ae. aegypti. Phylogenetic analysis showed that five of the six Ae. aegypti AQPs have high similarity to classical water-transporting AQPs of vertebrates. Using microarray, reverse transcription and real time PCR analysis we found that all six AQPs are expressed in distinct patterns in mosquito tissues/body parts. AaAQP1, 4, and 5 are strongly expressed in the adult female MT. RNAi-mediated knockdown of the MT-expressed mosquito AQPs resulted in significantly reduced diuresis.Conclusions/SignificanceOur results support the notion that AQP1, 4, and 5 function as water transporters in the MTs of adult female Ae. aegypti mosquitoes. Our results demonstrate the importance of these AQPs for mosquito diuresis after blood ingestion and highlight their potential as targets for the development of novel vector control strategies.

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Diuretic hormone 44 receptor in Malpighian tubules of the mosquito Aedes aegypti: evidence for transcriptional regulation paralleling urination

Cited by 31 publications

References 97 publications

A comprehensive transcriptomic view of renal function in the malaria vector, Anopheles gambiae

A comprehensive transcriptomic view of renal function in the malaria vector, Anopheles gambiae

Functional differences between two CRF-related diuretic hormone receptors in Drosophila

The Aquaporin Gene Family of the Yellow Fever Mosquito, Aedes aegypti

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