Diuretic and Laxative Activities of Moringa Oleifera Seeds and Pods in Mice

Abstract

Moringa oleifera (Family- Moringaceae) is a well-known vegetable for its health benefits for thousands of years having diverse nutritional bioactive constituents. Phytochemical analysis of ethanolic extract of this plant indicated the presence of carbohydrate, reducing sugar, combined reducing sugar, glycosides, tannins (absent in seeds), alkaloids, flavonoids, saponins and steroids. In diuretic activity test, the extract showed a significant (p<0.001) effect at the dose of 200 & 400 mg/kg body weight in production of total urine volume in comparison with standard drug furosemide (5 mg/kg). Upon electrolyte analysis of excreted urine, the extract was found to increase Na+, K+, Clexcretion and may act as loop diuretics. The pods and seeds extract showed significant (p<0.01) laxative effects in mice in terms of stool production with soft consistency at the dose of 200 & 400 mg/kg in comparison with standard drug bisacodyl (10 mg/kg). The most edible part of this plant is pods which showed better diuretic and laxative effects than seeds. So, the pods may be a good source of bioactive constituents which may be used as parent compound(s) for discovering better diuretic or laxative drugs.

Identification of Potential Diuretic and Laxative Drug Candidates from Avicennia officinalis L. Bark through In Vivo Mice Model Studies and In Vitro Gas Chromatography-Mass Spectrometry and Molecular Docking Analysis

“…Furthermore, some diuretic indices such as natriuretic, kaluretic, and saluretic were calculated to hypothesize the mechanism of such diuretic action and the results supported that the tested samples mechanistically are as like loop diuretic drugs ( Table 3 ). Some earlier scientific reports published that loop diuretics exert their action by modifying natriuresis and kaluresis through inhibition of Na + /K + /Cl − symporter in the nephron's loop of Henle [ 5 , 36 ]. Clinically, loop diuretics are prescribed for treating or managing patients with salt and water overload (pulmonary edema, cardiac edema, hypertension, etc.)…”

“…composition of the renal tubular fluid. These types of drugs are used in many clinical situations such as edema, hypertension, heart failure, renal obstruction, nephrotic syndrome, ascites, obesity, and pulmonary congestion [ 5 ]. Laxative drugs are used to manage constipation which is defined as the hardening of stool; resulting in obstruction in defecation.…”

Exploring the medicinal potential of the mangrove plant Phoenix paludosa: Insights into bacterial anti-biofilm and anti-quorum sensing activities

“…The mixture was kept away for 14 days, with occasional shaking and stirring. Subsequently, the solution was filtered using Whatman filter paper [17] . Following the evaporation of the filtrates, the resulting concentrates, with a yield of 3.84%, were recognized as crude ethanolic extracts derived from the leaves of P. paludosa.…”

Evaluation of Diuretic Property of Argemone mexicana along with Molecular Docking Study

“…The diuretic test procedure followed a pre-established methodology adapted by Golla and colleagues with minor modifications [47][48][49][50] .…”