Diuretic and laxative activities of Kandelia candel and Brownlowia tersa in experimental mice

Sarker, Mohua; Ahmed, Towkir; Islam, Md. Nazmul; Biswas, Bithi; Dev, Shrabanti; Bokshi, Bishwajit; Das, Asish Kumar; Biswas, Nripendra Nath

doi:10.22271/plants.2021.v9.i2a.1267

Cited by 3 publications

(4 citation statements)

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“…The diuretic test was performed in vivo using metabolic cages following the method of Sarker and her coworkers with minor modifications [ 4 ]. Briefly, the mice were kept starved for 18 h before starting the experiment.…”

Section: Methodsmentioning

confidence: 99%

“…have been reported to have many useful pharmacological properties such as anticancer, antibacterial, analgesic, anti-inammatory, antitumor, and antiviral [2,3]. e mangrove forest, Sundarbans, which is situated in the southern part of Bangladesh and possesses many plants of diverse ecological adaptive capacity due to high salinity, might be a new source of novel drug lead [4]. e drugs which promote the rate of urine flow are termed diuretics.…”

Section: Introductionmentioning

confidence: 99%

“…This uncomfortable public health problem may be alleviated by softening the stool and/or facilitating bowel movement [ 6 ]. Plants include a variety of chemicals (terpenoids, flavonoids, alkaloids, and others) that have laxative and diuretic properties [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Potential Diuretic and Laxative Drug Candidates from Avicennia officinalis L. Bark through In Vivo Mice Model Studies and In Vitro Gas Chromatography-Mass Spectrometry and Molecular Docking Analysis

Islam

Hasan

Dey

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Avicennia officinalis is a medicinal plant that has traditionally been used as a diuretic, anti-infective, and antiasthmatic. Our investigation was designed to explore the diuretic and laxative potentials of different fractions of this plant’s bark extract as well as the identification of possible drug candidates for the activity. Methods. Collected bark was extracted in ethanol and fractionated in different polar and nonpolar solvents, i.e., water, chloroform, ethyl acetate, and n-hexane. Phytoconstituents were identified following the published protocols and gas chromatography-mass spectrometry (GC-MS). In the diuretic test, Na+ and K+ ions were measured using a flame photometer whereas the Cl− ion content was measured by titrimetric method against AgNO3. In the laxative test, feces amount and consistency were also measured. Molecular docking analysis was conducted using the “Vina Wizard” program in PyRx-Python Prescription 0.8. Results. Phytochemical analysis indicated that alkaloids, tannins, flavonoids, saponins, glycosides, and terpenoids were detected in the most bioactive crude extracts, whereas alkaloids, terpenoids, saponins, and gums were found in bioactive n-hexane fraction and steroids, glycosides, and terpenoids were found positive in chloroform fraction. Almost all the fractions demonstrated a dose-dependent increment of stool production with a soft consistency; however, the chloroform fraction was found to be the most active ( p < 0.001). The crude extract and n-hexane fractions significantly increased ( p < 0.01) the urinary output at the dose of 200 and 400 mg/kg. The concentrations of Na+, K+, and Cl− in collected urine were found to be more compared with the control group. The GC-MS analysis identified seven compounds in bioactive n-hexane fraction (phenolic and ester-type mainly) whereas seven other compounds (acidic and ester-type mainly) were identified in chloroform fraction. In molecular docking, two drug candidates of this extract (2,4-bis(2-phenylpropan-2-yl)phenol and 2-[4-[2-(dimethylamino)-2-oxo-1,1-diphenylethyl]phenyl]-2-phenylacetic acid) showed excellent binding affinity with the receptor compared with furosemide. Conclusion. A. officinalis bark might be a potential source of bioactive compounds for treating hypertension, edema, and constipation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of Potential Diuretic and Laxative Drug Candidates from Avicennia officinalis L. Bark through In Vivo Mice Model Studies and In Vitro Gas Chromatography-Mass Spectrometry and Molecular Docking Analysis

Islam

Hasan

Dey

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Nonpolar molecules, more soluble in n-hexane than water, dissolved in the top layer collected as n-hexane, while the bottom layer contained water. The water layer was transferred to a new funnel and the process repeated using dichloromethane and ethyl acetate [18] . The water fraction was dried with a Labocon freeze-dryer (Model: LFD-BT-104), and a rotary evaporator removed organic solvents from each fraction.…”

Section: Liquid-liquid Partitioning Of Crude Extractmentioning

confidence: 99%

Exploring the medicinal potential of the mangrove plant Phoenix paludosa: Insights into bacterial anti-biofilm and anti-quorum sensing activities

Ankhi,

Islam,

Biswas

et al. 2024

J. Med. Plants Stud.

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Evaluation of Diuretic Property of Argemone mexicana along with Molecular Docking Study

Sardar,

Devnath,

Biswas

et al. 2023

Jordan j. pharm. sci.

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Background: Argemone mexicana L. (A. mexicana) has traditionally been used to treat hypertension, urinary issues, and constipation. In this study, we assessed the diuretic activity of the ethanolic crude extract of A. mexicana. Methods: Phytochemical tests were conducted using standard reagents and methods widely accepted in the field. The diuretic test was performed in metabolic cages using a mouse model, with furosemide (5 mg/kg) as the standard drug. Molecular docking was carried out in PyRx using Autodock Vina 4.2. To assess the stability of the protein-ligand complexes formed during docking, we conducted molecular dynamics (MD) simulations for the β-amyrin-6PZT protein complex and the furosemide-6PZT protein complex. Various parameters, including RMSD, RMSF, Rg, SASA, and hydrogen bonds, were calculated for all protein-ligand complexes. Results: Phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, steroids, terpenoids, saponins, and tannins in the crude extract. The crude extract exhibited significant (p < 0.05) diuretic activity compared to the control group. Furthermore, we detected the presence of electrolytes (Na+, K+, and Cl-) in the urine of mice treated with the crude extract. In the molecular docking study, among the eighteen compounds studied, β-amyrin displayed superior diuretic potential. The results of the molecular dynamics simulation for the β-amyrin-6PZT protein complex indicated good stability, comparable to the reference drug, furosemide. Conclusion: The crude extract of A. mexicana demonstrates significant diuretic effects that could be valuable for edema treatment. The findings from the molecular docking and molecular dynamics simulations suggest the potential for further research in developing a novel drug.

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Diuretic and laxative activities of Kandelia candel and Brownlowia tersa in experimental mice

Cited by 3 publications

References 0 publications

Identification of Potential Diuretic and Laxative Drug Candidates from Avicennia officinalis L. Bark through In Vivo Mice Model Studies and In Vitro Gas Chromatography-Mass Spectrometry and Molecular Docking Analysis

Identification of Potential Diuretic and Laxative Drug Candidates from Avicennia officinalis L. Bark through In Vivo Mice Model Studies and In Vitro Gas Chromatography-Mass Spectrometry and Molecular Docking Analysis

Exploring the medicinal potential of the mangrove plant Phoenix paludosa: Insights into bacterial anti-biofilm and anti-quorum sensing activities

Evaluation of Diuretic Property of Argemone mexicana along with Molecular Docking Study

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