Dithiothreitol prevents age-associated decrease in oocyte/conceptus viability in vitro

Tarı́n, Juan J.; Ten, Jorge; Vendrell, Francisco J.; Cano, Antonio

doi:10.1093/humrep/13.2.381

Cited by 74 publications

(64 citation statements)

References 31 publications

(19 reference statements)

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“…The most prominent manifestations of aged oocytes include an increased susceptibility to activating stimuli (Kubiak 1989, Lan et al 2004, a decrease in MPF activity (Wu et al 1997, Xu et al 1997), a partial exocytosis of cortical granules (Szollosi 1971, Ducibella et al 1990, signs of apoptotic cell death such as increased cytoplasmic and DNA fragmentation (Gordo et al 2000) and decreased levels of BCL2 proteins (Gordo et al 2002), the onset of anaphase II (Xu et al 1997, Abbott et al 1998, and impaired embryo development following fertilization ( Juetten & Bavister 1983, Tesarik 1993, Tarin et al 1998. In addition, an increased acetylation on some lysines of histones has also been observed in aged mouse oocytes (Huang et al 2007).…”

Section: Discussionmentioning

confidence: 99%

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Pyruvate prevents aging of mouse oocytes

Liu

Wu²,

Lan³

et al. 2009

Reproduction

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Inhibiting oocyte aging is important not only for healthy reproduction but also for the success of assisted reproduction techniques. Although our previous studies showed that cumulus cells accelerated aging of mouse oocytes, the underlying mechanism is unknown. The objective of this paper was to study the effects of pyruvate and cumulus cells on mouse oocyte aging. Freshly ovulated mouse cumulus-oocyte complexes (COCs) or cumulus-denuded oocytes (DOs) were cultured in Chatot-Ziomek-Bavister (CZB) medium or COC-conditioned CZB medium supplemented with different concentrations of pyruvate before being examined for aging signs and developmental potential. Pyruvate supplementation to CZB medium decreased rates of ethanol-induced activation in both COCs and DOs by maintaining their maturation-promoting factor activities, but more pyruvate was needed for COCs than for DOs. Addition of pyruvate to the COC-conditioned CZB also alleviated aging of DOs. Observations on cortical granules, level of BCL2 proteins, histone acetylation, intracellular concentration of glutathione, and embryo development all confirmed that pyruvate supplementation inhibited aging of mouse oocytes. It is concluded that the aging of mouse oocytes, facilitated by culture in COCs, can be partially prevented by the addition of pyruvate to the culture medium.

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Section: Discussionmentioning

confidence: 99%

“…Postovulatory aging of oocytes significantly affects embryonic development (Juetten & Bavister 1983, Tesarik 1993, Tarin et al 1998). …”

Section: Introductionmentioning

confidence: 99%

Pyruvate prevents aging of mouse oocytes

Liu

Wu²,

Lan³

et al. 2009

Reproduction

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“…Early research by Tarin et al (1998) demonstrated that L-ascorbic acid (vitamin C) and 6-methoxy-2,5,7,8-tetramethylchlormane-2-carboxylic acid (trolox/vitamin E) could not significantly improve fertilization rate nor prevent fragmentation associated with post-ovulatory ageing, while b-mercaptoethanol and L-cysteine in fact decreased the likelihood of aged oocytes reaching the blastocyst stage following fertilization. However, this research also established that addition of the reducing agent dithiothreitol (DTT) to oocyte culture medium resulted in increased fertilization and blastocyst formation rates, potentially by protecting free thiol groups within these 'ageing' oocytes from becoming oxidized.…”

Section: Antioxidantsmentioning

confidence: 99%

Oxidative stress and ageing of the post-ovulatory oocyte

2013

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With extended periods of time following ovulation, the metaphase II stage oocyte experiences deterioration in quality referred to as post-ovulatory oocyte ageing. Post-ovulatory ageing occurs both in vivo and in vitro and has been associated with reduced fertilization rates, poor embryo quality, post-implantation errors and abnormalities in the offspring. Although the physiological consequences of post-ovulatory oocyte ageing have largely been established, the molecular mechanisms controlling this process are not well defined. This review analyses the relationships between biochemical changes exhibited by the ageing oocyte and the symptoms associated with the ageing phenotype. We also discuss molecular events that are potentially involved in orchestrating post-ovulatory ageing with a particular focus on the role of oxidative stress. We propose that oxidative stress may act as the initiator for a cascade of events that create the aged oocyte phenotype. Specifically, oxidative stress has the capacity to cause a decline in levels of critical cell cycle factors such as maturation-promoting factor, impair calcium homoeostasis, induce mitochondrial dysfunction and directly damage multiple intracellular components of the oocyte such as lipids, proteins and DNA. Finally, this review addresses current strategies for delaying post-ovulatory oocyte ageing with a particular focus on the potential use of compounds such as caffeine or selected antioxidants in the development of more refined media for the preservation of oocyte integrity during IVF procedures.

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“…L-cystine, a component of GSH, and -mercaptoethanol decreases oocyte quality to develop to the blastocyst stage, whereas dithiothreitol (DTT) enhances the fertilization rate and the developmental capacity of oocytes (Tarin et al 1998b). Moreover, DTT supports embryonic integrity regarding cell number in inner cell mass cells (Rausell et al 2007).…”

Section: Other Compounds That Affect Oocyte Agingmentioning

confidence: 99%