Dithionite Tube Test—A Rapid, Inexpensive Technique for the Detection of Hemoglobin S and Non-S Sickling Hemoglobin

Nalbandian, Robert M.; Nichols, Bruce M.; Camp, Frank R.; Lusher, Jeanne M.; Conte, Nicholas F.; Henry, Raymond L.; Wolf, Paul L.

doi:10.1093/clinchem/17.10.1028

Cited by 23 publications

(11 citation statements)

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“…Sickle cell screening was also done for participants who provided additional consent. Sickle cell screening was carried out using the solubility test [Biolab, India] [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

Knowledge, perception and practices towards sickle cell disease: a community survey among adults in Lubaga division, Kampala Uganda

Tusuubira¹,

Nakayinga²,

Mwambi³

et al. 2018

BMC Public Health

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BackgroundWorldwide, the burden of Sickle Cell disease (SCD) has not been amply addressed. In Africa, Uganda has the 5th highest burden, a situation aggravated by limited and inaccessible formal social support structures to aid patients and families cope better with the psychosocial burden of SCD. In addition, this has been coupled with stigmatization and discrimination of people living with sickle cell disease causing isolation from family and society.MethodThis cross sectional study therefore set out to determine the attitudes, perception and level of awareness towards Sickle Cell disease in Ugandan communities. The study used an interviewer administered questionnaires to collect the data.ResultsOut of 110 people sampled; 91.2% of the respondents had ever heard of SCD with the highest proportion 38.7% hearing of SCD from friends and family. Close to half of the respondents 48% knew that SCD is inherited, however a large proportion 44.2% did not know the cause of SCD. However, 68.7% of the respondents said they cannot marry a person with SCD.ConclusionThe study results indicate that more effort needs to be done to promote sickle cell awareness in Uganda communities with emphasis on the inclusion of sickle cell in health education campaigns.

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“…Sickle cell screening was also done for participants who provided additional consent. Sickle cell screening was carried out using the solubility test [Biolab, India] [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

Knowledge, perception and practices towards sickle cell disease: a community survey among adults in Lubaga division, Kampala Uganda

Tusuubira¹,

Nakayinga²,

Mwambi³

et al. 2018

BMC Public Health

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“…Haemoglobin electrophoretic variants with mobility indistinguishable from S, C, B, or F on alkaline starch-gel electrophoresis (Efremov et aE. 1969) were considered variants of the p-S-y region, providing that in the case of variants with mobility indistinguishable from S they were also positive in the dithionite tube test (Nalbandian et al 1971). For 68 of the 72 heterozygous individuals the spouse was also typed.…”

mentioning

confidence: 99%

The linkage relationships of the haemoglobin beta, delta and alpha loci with 34 genetic marker systems

Weitkamp

Stamatoyannopoulos

Rowley

et al. 1977

Annals of Human Genetics

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“…Further identification of HbS was made by a precipitation test [12]. Heterozygous thalassaemia was diagnosed by the typical haematological (microcytosis) and electrophoretic patterns: high HbA2 (9%) and HbF (3%), and the alkaline denaturation test [13].…”

Section: Laboratory Methodsmentioning

confidence: 99%

Haemoglobinopathies: a pitfall in the assessment of glycosylated haemoglobin by ion-exchange chromatography

et al. 1984

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Total glycosylated haemoglobin was determined by a minicolumn ion-exchange chromatography technique (Bio-Rad) and correlated with the mean of fasting and post-prandial blood glucose values for the preceding 6 weeks. In 360 diabetic subjects, free of congenital haemoglobinopathies and other detected causes of haemoglobin A1 misinterpretation (reference diabetic group), a highly significant correlation was established between haemoglobin A1 and glucose (y = 0.54 X +4.91; r = 0.791; p less than 0.01). In 28 of the 29 patients with heterozygous haemoglobinopathies (HbS, C, D, E), the apparent haemoglobin A1 values were lower than expected according to the 95% confidence limits of the diabetic reference group. The apparent haemoglobin A1 value was above these limits in patient 29, with beta thalassaemia. Patients with inappropriate glycosylated haemoglobin values should be investigated for causes of haemoglobin A1 misinterpretation, in particular, haemoglobinopathies.

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Dithionite Tube Test—A Rapid, Inexpensive Technique for the Detection of Hemoglobin S and Non-S Sickling Hemoglobin

Cited by 23 publications

References 0 publications

Knowledge, perception and practices towards sickle cell disease: a community survey among adults in Lubaga division, Kampala Uganda

Knowledge, perception and practices towards sickle cell disease: a community survey among adults in Lubaga division, Kampala Uganda

The linkage relationships of the haemoglobin beta, delta and alpha loci with 34 genetic marker systems

Haemoglobinopathies: a pitfall in the assessment of glycosylated haemoglobin by ion-exchange chromatography

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