Diterpenoids Isolated from Podocarpus macrophyllus Inhibited the Inflammatory Mediators in LPS-Induced HT-29 and RAW 264.7 Cells

Kim, ChoEen; Le, DucDat; Lee, Mina

doi:10.3390/molecules26144326

Cited by 9 publications

(4 citation statements)

References 34 publications

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“…Our results, in line with the findings described by other authors, showed that HT-29 cells, subjected to LPS stimuli, release in vitro inflammatory cytokines, including IL-8, TNF-α, and IL-6 [ 47 , 48 ]. The anti-inflammatory activity of PD was evaluated by pretreating or treating HT-29 cells in combination with LPS.…”

Section: Discussionsupporting

confidence: 93%

Influence of Polydatin on the Tumor Microenvironment In Vitro: Studies with a Colon Cancer Cell Model

Gregorio

Krasnowska

Zonfrillo

et al. 2022

IJMS

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The tumor microenvironment of colon carcinoma, the site at which tumor cells and the host immune system interact, is influenced by signals from tumor cells, immunocompetent cells, and bacterial components, including LPS. A large amount of LPS is available in the colon, and this could promote inflammation and metastasis by enhancing tumor cell adhesion to the endothelium. Polydatin (PD), the 3-β-D-glucoside of trans-resveratrol, is a polyphenol with anti-cancer, anti-inflammatory, and immunoregulatory effects. This study was designed to explore whether PD is able to produce antiproliferative effects on three colon cancer lines, to reduce the expression of adhesion molecules that are upregulated by LPS on endothelial cells, and to decrease the proinflammatory cytokines released in culture supernatants. Actually, we investigated the effects of PD on tumor growth in a coculture model with human mononuclear cells (MNCs) that mimics, at least in part, an in vitro tumor microenvironment. The results showed that PD alone or in combination with MNC exerts antiproliferative and proapoptotic effects on cancer cells, inhibits the production of the immunosuppressive cytokine IL-10 and of the proinflammatory cytokines upregulated by LPS, and reduces E-selectin and VCAM-1 on endothelial cells. These data provide preclinical support to the hypothesis that PD could be of potential benefit as a therapeutic adjuvant in colon cancer treatment and prevention.

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Section: Discussionsupporting

confidence: 93%

Influence of Polydatin on the Tumor Microenvironment In Vitro: Studies with a Colon Cancer Cell Model

Gregorio

Krasnowska

Zonfrillo

et al. 2022

IJMS

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“…Recent studies have found that CASP activation can be observed in tumor cell apoptosis. CASP refers to a family of cysteine aspartate-specific proteases, and these proteases are the main enzymes that perform cell apoptosis and are involved in the occurrence and development of various diseases, particularly tumors and autoimmune diseases[ 26 ]. As a key protease, CASP8 participates in the transmission of exogenous apoptosis signals in mammals, and CASP9 plays a crucial role in the mitochondria-mediated endogenous apoptosis pathway[ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology and molecular docking reveal zedoary turmeric-trisomes in Inflammatory bowel disease with intestinal fibrosis

Ji¹,

Dai²,

Wen³

et al. 2022

WJCC

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BACKGROUND Inflammatory bowel disease (IBD) is a complex chronic IBD that is closely associated with risk factors such as environment, diet, medications and lifestyle that may influence the host microbiome or immune response to antigens. At present, with the increasing incidence of IBD worldwide, it is of great significance to further study the pathogenesis of IBD and seek new therapeutic targets. Traditional Chinese medicine (TCM) treatment of diseases is characterized by multiple approaches and multiple targets and has a long history of clinical application in China. The mechanism underlying the effect of zedoary turmeric-trisomes on inducing mucosal healing in IBD is not clear. AIM To explore the effective components and potential mechanism of zedoary turmeric-trisomes in the treatment of IBD with intestinal fibrosis using network pharmacology and molecular docking techniques. METHODS The chemical constituents and targets of Rhizoma zedoary and Rhizoma sanarum were screened using the TCMSP database. The GeneCards database was searched to identify targets associated with intestinal fibrosis in IBD. The intersection of chemical component targets and disease targets was obtained using the Venny 2.1 online analysis platform, and the common targets were imported into the STRING 11.0 database to construct a protein interaction regulatory network. A “zedoary turmeric-trisomes-chemical composition-target-disease” network diagram was subsequently constructed using Cytoscape 3.7.2 software, and the topological properties of the network were analyzed using the “Network Analysis” plug-in. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the common targets were performed using the DAVID 6.8 database to elucidate the mechanism of zedoary turmeric-trisomes in the treatment of IBD. Subsequently, molecular docking of the compounds and targets with the highest intermediate values in the “zedoary turmeric-trisomes-chemical composition-target-disease” network was performed using Sybyl-x 2.1.1 software. RESULTS A total of 5 chemical components with 60 targets were identified, as well as 3153 targets related to IBD and 44 common targets. The protein-protein interaction network showed that the core therapeutic targets included JUN, MAPK14, CASP3, AR, and PTGS2. The GO enrichment analysis identified 759 items, and the KEGG enrichment analysis yielded 52 items, including the cancer pathway, neuroactive ligand-receptor interaction, hepatitis B, and the calcium signaling pathway, reflecting the complex biological processes of the multicomponent, multitarget and multipathway treatment of diseases with zedoary turmeric-trisomes. Molecular docking showed that the compound bonded with the target through hydrogen bond interactions and exhibited good docking activity. CONCLUSION This study identified the potential m...

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“…Equal volumes of both supernatant and Griess reagent were mixed in a dark condition at room temperature for 10 min. Nitric oxide concentration was detected by measuring the absorbance of mixture at 540 nm using an ELISA plate reader (aBMGLABTECH's FLUOstar Omega microplate reader (Ortenberg, Germany), and the percentage of inhibition was calculated based on the following equation (Kim et al, 2021;Ahmed et al, 2022): Inhibition (%) = (absorbance of control cellsabsorbance of sample) x 100/absorbance of control.…”

Section: C) Measurement Of Nitric Oxidementioning

confidence: 99%

Antiradical and anti-inflammatory activity of Saccharomyces cerevisiae- mediated selenium nanoparticles

Fath-Alla,

Khalil,

Mohamed

et al. 2024

Egypt. J. Bot.

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HE PRESENT research study aimed for the biosynthesis of selenium nanoparticles (SeNPs) using Saccharomyces cerevisiae (baker's yeast) as a sustainable, green, eco-friendly, and economically suitable source. The yeast-mediated SeNPs were characterized to detect their physicochemical properties by using UV-Vis, TEM, DLS, FTIR, and XRD analyses. The biosynthesized SeNPs were spherical in shape with sizes ranging from 34 to 125 nm, carrying a negative charge that equals -22.4 mV, and had an amorphous nature. FTIR indicated that SeNPs were surrounded with bioactive proteins as reducing and capping agents produced by yeast cells. Various biological capacities of biosynthesized SeNPs were measured including cytotoxic, antiradical, antiinflammatory, and antimicrobial activities. The cytotoxicity results showed that the yeast-mediated SeNPs are nontoxic particles with IC 50 value above 300 µg/mL. Therefore, they can be applied as a safe and green therapeutic agent. An antiradical assay revealed that SeNPs had the scavenging ability of DPPH. In addition, anti-inflammatory tests ensured the capacity of SeNPs to inhibit nitric oxide released from macrophage cells due to induced inflammation. Both antiradical and anti-inflammatory actions were shown at noncytotoxic SeNP concentrations. The highest antiradical activity (63.26%) was shown at 150 µg/mL SeNPs, and the highest anti-inflammatory action (41.34%) was observed at 100 µg/mL SeNPs. Subsequently, yeast-derived SeNPs are a safe, alternative, and sustainable agent that can be used as an antiradical and anti-inflammatory drug at their noncytotoxic levels (1-300 µg/mL).

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Diterpenoids Isolated from Podocarpus macrophyllus Inhibited the Inflammatory Mediators in LPS-Induced HT-29 and RAW 264.7 Cells

Cited by 9 publications

References 34 publications

Influence of Polydatin on the Tumor Microenvironment In Vitro: Studies with a Colon Cancer Cell Model

Influence of Polydatin on the Tumor Microenvironment In Vitro: Studies with a Colon Cancer Cell Model

Network pharmacology and molecular docking reveal zedoary turmeric-trisomes in Inflammatory bowel disease with intestinal fibrosis

Antiradical and anti-inflammatory activity of Saccharomyces cerevisiae- mediated selenium nanoparticles

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