Diterpenoids from Euphorbia gedrosiaca as Potential Anti-Proliferative Agents against Breast Cancer Cells

Abstract

Isolated diterpenes from various species of Euphorbia are important compounds for drug discovery with a broad spectrum of structures and biological effects. In this study, Euphorbia gedrosiaca, one of the endemic species of Iran, was analyzed in terms of the presence and structural determination of diterpenoid compounds. They were extracted with dichloromethane/acetone (2:1) from aerial parts of this plant and purified by chromatographic methods such as MPLC and HPLC. Four premyrsinane compounds and one myrsinane diterpene were isolated from Euphorbia gedrosiaca. They were characterized by extensive 1D and 2D NMR and HRMS analyses. Additionally, their activities were evaluated against two breast cancer cell lines, MDA-MB-231 and MCF-7, by MTT proliferation assay. They exhibited cytotoxic effects in a dose-dependent manner with promising results, which can help to find possible therapeutic application of diterpenoids in breast cancer treatment.

Myrsinane-Type Diterpenes: A Comprehensive Review on Structural Diversity, Chemistry and Biological Activities

“…These findings demonstrated that Myrs and 84 clearly induce more cell death than Cyclomyrsinanes 53 and 85 [54]. A very recent study led to the isolation of four premyrsinane diterpenes myrsinane diterpene [76]. Premyrsinanes 120, 131, 132, and 136 had previou isolated from E. sanctae-catharinae [77] and E. pithyusa [69].…”

“…When tested for cytotoxicity against these two cell lines, Compound 49 showed less potency than the other four diterpenes. However, the IC 50 value of 33.7 µM can be considered modest on the MDA-MB-231 cell line [ 76 ].…”

“…Several cyclomyrsinane (73)(74)(75)(76)(77)(78) and premyrsinane diterpenes (79-81) (Figure 14) were isolated from the acetone/dichlorometane extract of the aerial parts of E. sogdiana Popov [52]. The in vitro cytotoxic activity of the isolated diterpenes was screened against EJ-138 bladder carcinoma and Jurkat T-leukemia cell lines.…”

“…When tested for cytotoxicity against these two cell lines, Comp showed less potency than the other four diterpenes. However, the IC50 value of can be considered modest on the MDA-MB-231 cell line [76]. A very recent study led to the isolation of four premyrsinane diterpenes and one myrsinane diterpene [76].…”

A Biomimetic Approach to Premyrsinane-Type Diterpenoids: Exploring Microbial Transformation to Enhance Their Chemical Diversity

“…Studies of the biological activities of premyrsinanes are scarce in the literature; this is likely because of the reduced amount of the isolated compounds [ 10 ]. Nevertheless, several studies have reported that premyrsinanes possess promising anticancer [ 17 , 18 , 19 ], multidrug resistance reversal [ 20 ], and anti-inflammatory activities, inhibiting the lipopolysaccharide-induced NO production [ 21 ]. Furthermore, their potential biosynthetic precursors, euphoboetirane A ( 3 ) and epoxyboetirane A ( 4 ), have demonstrated a promising activity as promoters of neural stem cells (NSC) proliferation [ 22 ].…”