Diterpenoid Alkaloids from the Aerial Parts of Aconitum flavum Hand.-Mazz

Abstract

Sixteen diterpenoid alkaloids (DAs), including six aconitine-type alkaloids (5 and 9 − 13), seven 7,17-seco-aconitine-type alkaloids (1 − 4, 6 − 8), two napelline-type alkaloids (14 and 15) as well as one veatchine-type alkaloid (16), were isolated from the aerial parts of Aconitum flavum Hand.-Mazz. In which, flavumolines A − D (1 − 4) were four new ones, and flavumoline E (5) was reported as natural compound for the first time. Their chemical structures were elucidated by the analysis of extensive spectroscopic data. The inhibitory activities of these isolates on Cav3.1 low voltage-gated Ca2+ channel, NO production in LPS-activated RAW264.7cells, five human tumor cell lines, as well as acetylcholinesterase (AChE) were tested.

Tandem mass tag labeled quantitative proteomic analysis of differential protein expression on total alkaloid of Aconitum flavum Hand.-Mazz. against melophagus ovinus

“…The AFHM total alkaloids were detected by HPLC/QTOF-MS ( Figure 1 ), and the mass/charge ratio and molecular weight of the detected components were compared with the database of traditional Chinese medicine. There were 11 alkaloids ( Table 1 ), namely 6-O-demethylneoline, songorine, 12-epi-napelline, neoline, 10-hydroxyneoline, jesaconine ( 25 ), benzoylaconitin, 3-deoxyaconitine ( 26 ), mesaconitine, acetylaconitine, and aconitine ( 27 ), which were consistent with existing research reports. The contents of aconitine and 3-deoxyaconitine in total alkaloids were determined by HPLC.…”

Species Identification and Genetic Diversity Analysis of Medicinal Plants Aconitum pendulum Busch and Aconitum flavum Hand.-Mazz.

“…In our laboratory, genomic DNA was extracted using the modified CTAB method [ 55 , 56 ] in 2021 and 2022. The concentration and purity of the extracted DNA were determined by spectrophotometer using NanoDrop 2000c (Thermo Scientific, Waltham, MA, USA) equipment and 0.8% agarose gel.…”

Exploring the Biomedical Potential of Terpenoid Alkaloids: Sources, Structures, and Activities

“…Franchetine-type C19-DAs are distinguished from aconitines by an additional ether between C-6 and C-17. Ten novel alkaloids (159-168) are classified as franchetine-type C19-DAs [43,56,76]. The plant sources are shown in Table 7.…”

“…Detailed chemical structures are shown in Figure 7. Compounds 159, 161-166, and 168 have a double bond between C-7 and C-8, whereas compounds 160 and 167 have a 7,8-epoxy unit [43,56,76]. In addition, Compounds 161 and 163 have a characteristic hydroxyl group at C-16 instead of the methoxy group joint in DAs.…”