Disulfiram Suppresses Invasive Ability of Osteosarcoma Cells Via the Inhibition of MMP-2 and MMP-9 Expression

Cho, Hyun-Ji; Lee, Tae-Sung; Park, Jae-Bok; Park, Kwan‐Kyu; Choe, Jung‐Yoon; Sin, Doo-Il; Park, Yoon-Yub; Moon, Yun-Seob; Lee, Kwang-Gill; Yeo, Joo‐Hong; Han, Sang‐Mi; Cho, Young-Su; Choi, Myeong-Rak; Park, Nam‐Gyu; Lee, Yun-Sik; Chang, Young‐Chae

doi:10.5483/bmbrep.2007.40.6.1069

Cited by 78 publications

(74 citation statements)

References 23 publications

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“…After 24 h, the cells on the apical side were wiped off with a Q-tip. Cells on the bottom of the filter were stained with hematoxylin (SigmaAldrich) and counted (three fields of each triplicate filter) with an inverted microscope (20).…”

Section: Matrigel Invasion Assaymentioning

confidence: 99%

Anti-invasive activity of histone deacetylase inhibitors via the induction of Egr-1 and the modulation of tight junction-related proteins in human hepatocarcinoma cells

Kim

Choi

Choi³

et al. 2009

BMB Reports

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Section: Matrigel Invasion Assaymentioning

confidence: 99%

Anti-invasive activity of histone deacetylase inhibitors via the induction of Egr-1 and the modulation of tight junction-related proteins in human hepatocarcinoma cells

Kim

Choi

Choi³

et al. 2009

BMB Reports

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“…Soluble MICA and B are produced by the proteolytic cleavage of cell-surface MICA and MICB by proteases, including matrix metalloproteinases (MMPs) (9,10), and human osteosarcomas have been reported to produce MMP-2 and -9 (33,34). Since it has been reported that HDAC inhibitors including VPA down-regulate the activity of MMPs in gastric, thyroid and lung cancer cells (36)(37)(38), the inhibitory effect of VPA on the production of soluble MICs may be ascribed to its inhibitory action on the activity of MMPs.…”

Section: Discussionmentioning

confidence: 99%

Valproic acid cooperates with hydralazine to augment the susceptibility of human osteosarcoma cells to Fas- and NK cell-mediated cell death

et al. 2012

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Abstract.We investigated the effects of valproic acid (VPA), a histone deacetylase inhibitor, in combination with hydralazine, a DNA methylation inhibitor, on the expression of cell-surface Fas and MHC-class I-related chain molecules A and B (MICA and B), the ligands of NKG2D which is an activating receptor of NK cells, and on production of their soluble forms in HOS, U-2 OS and SaOS-2 human osteosarcoma cell lines. We also examined the susceptibility of these cells to Fas-and NK cellmediated cell death. VPA did not increase the expression of Fas on the surface of osteosarcoma cells, while hydralazine did, and the combination of VPA with hydralazine increased the expression of cell-surface Fas. In contrast, the combination of VPA with hydralazine did not increase the production of soluble Fas by osteosarcoma cells. Both VPA and hydralazine increased the expression of cell-surface MICA and B in osteosarcoma cells, and their combination induced a greater increase in their expression. VPA inhibited the production of both soluble MICA and MICB by osteosarcoma cells while hydralazine produced no effect. Both VPA and hydralazine enhanced the susceptibility of osteosarcoma cells to Fas-and NK cell-mediated cell death and the combination of VPA with hydralazine further enhanced the effects. The present results suggest that combined administration of VPA and hydrazine is valuable for enhancing the therapeutic effects of immunotherapy for osteosarcomas.

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“…The aldehyde dehydrogenase inhibitory activity of disulfiram is the basis of its therapeutic efficacy against alcoholism (Edwards et al, 1991;Cvek & Dvorak, 2007). Disulfiram also suppresses matrix metalloproteinase (MMP) expression in osteosarcoma cells through modulation of NF-kB and activator protein-1, and possibly its metal chelating properties (Cho et al, 2007).…”

Section: Molecular and Cellular Effectsmentioning

confidence: 99%

Dithiocarbamate Toxicity - An Appraisal

Rath¹,

Rasaputra²,

Liyanage³

et al. 2011

Pesticides in the Modern World - Effects of Pesticides Exposure

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Disulfiram Suppresses Invasive Ability of Osteosarcoma Cells Via the Inhibition of MMP-2 and MMP-9 Expression

Cited by 78 publications

References 23 publications

Anti-invasive activity of histone deacetylase inhibitors via the induction of Egr-1 and the modulation of tight junction-related proteins in human hepatocarcinoma cells

Anti-invasive activity of histone deacetylase inhibitors via the induction of Egr-1 and the modulation of tight junction-related proteins in human hepatocarcinoma cells

Valproic acid cooperates with hydralazine to augment the susceptibility of human osteosarcoma cells to Fas- and NK cell-mediated cell death

Dithiocarbamate Toxicity - An Appraisal

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