Disturbances of Reproduction and Ovarian Changes in the Guinea-Pig in Relation to Vitamin C Deficiency

Kramer, M. M.; Harman, Mary T.; Brill, Alice K.

doi:10.1152/ajplegacy.1933.106.3.611

Cited by 42 publications

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“…These observations of ovarian chances in the vitamin Cdeficient animals of this study seemed to confirm the work of Kramer, Harman and Brill (1933), and they indicate that the…”

Section: Blood Sodium Levelsupporting

confidence: 90%

“…As an example, Turner (1948, p. 230, 232) reports that adrenalectomy causes a decrease in the volume of blood plasma and an interruption in reproductive functions, while for vitamin C..deficieney, Harman and Kordisch (1945) report. a circulatory chance that may be due to a in blood volume, and Goettsch (1930) and Kramer, Harman and Brill (1933) report interference with reproduction and degenerative chances in ovaries and testes.…”

Section: Introduction Ant? Review Cf Iteraturementioning

confidence: 99%

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An Investigation into the Possible Relationship between Vitamin C and the Adrenal Cortex of the Guinea Pig

Harman¹,

Bascom²

1951

Transactions of the Kansas Academy of Science (1903-)

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“…These observations of ovarian chances in the vitamin Cdeficient animals of this study seemed to confirm the work of Kramer, Harman and Brill (1933), and they indicate that the…”

Section: Blood Sodium Levelsupporting

confidence: 90%

Section: Introduction Ant? Review Cf Iteraturementioning

confidence: 99%

An Investigation into the Possible Relationship between Vitamin C and the Adrenal Cortex of the Guinea Pig

Harman¹,

Bascom²

1951

Transactions of the Kansas Academy of Science (1903-)

Self Cite

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“…However, a reduced level of ascorbic acid in the follicular fluid of acyclic buffaloes might possibly lead to follicular atresia as observed in the present study, as a consequence of inadequate protection of maturing granulosa cells, oocytes, and steroids from the damaging effects of reactive oxygen species (Tilly and Tilly 1995). Ascorbic acid has also been implicated in the regulation of the follicular basement membrane and its deficiency causes degeneration of the membrane (Kramer et al 1933;Espey and Coons 1976). It has been speculated that growing follicle would have a high requirement for ascorbic acid if it is to produce sufficient basal lamina components to maintain expansion of the membrane during development (Murray et al 2001).…”

Section: Discussionmentioning

confidence: 55%

Follicular characteristics and intrafollicular concentrations of nitric oxide and ascorbic acid during ovarian acyclicity in water buffalo (Bubalus bubalis)

Khan

Das

2011

Trop Anim Health Prod

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The objective of this study was to examine the follicular characteristics and intrafollicular concentrations of nitric oxide and ascorbic acid during ovarian acyclicity in buffaloes. Ovaries were collected from 56 acyclic and 95 cyclic buffaloes at slaughter, surface follicle number was counted and follicles were classified into small (5.0-6.9 mm), medium (7.0-9.9 mm), and large (≥ 10.0 mm) size categories based on their diameter. Follicular fluid was aspirated and assayed for nitric oxide, ascorbic acid, estradiol, and progesterone. Acyclic buffaloes had a higher (P<0.05) number of medium-sized follicles and a lower (P<0.001) number of large follicles than the cyclic ones. In acyclic animals, the number of large follicles was lower (P<0.01) than in medium size category which in turn was lower (P<0.001) than the number of small follicles. In contrast, the number of medium and large follicles was not different (P>0.05) in the cyclic control. However, the number of small-sized follicles was higher (P<0.001) compared to the other two categories. The incidence of large-sized follicles was lower (P<0.05) in acyclic buffalo population compared to the cyclic control. Evaluation of estrogenic status demonstrated that all the follicles of acyclic buffaloes are estrogen-inactive (E (2)/P (4) ratio<1). Small- and medium-sized follicles of acyclic buffaloes had higher concentrations of nitric oxide (P<0.05 and P<0.001, respectively) and lower concentrations of ascorbic acid (P<0.05 and P<0.01, respectively) than the corresponding size estrogen-active follicles of their cyclic counterparts. In conclusion, this study indicates that follicular development continues during acyclicity in buffaloes. Although follicles in some acyclic buffaloes attain a size corresponding to morphological dominance, they are unable to achieve functional dominance, perhaps due to an altered balance of intrafollicular nitric oxide and ascorbic acid and, as a result, these follicles instead of progressing to ovulation undergo atresia.

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“…In diabetes mellitus, for example, blood levels of DHAA are persistently high [2] and may in part reflect the inhibition of cellular DHAA uptake by increased serum glucose, which in the diabetic patient can reach concentrations as high as 20 mM [48]. Ample levels of ascorbic acid are crucial for fertility, as reviewed previously [49].…”

Section: Discussionmentioning

confidence: 96%

Accumulation of Ascorbate by Endocrine-Regulated and Glucose-Sensitive Transport of Dehydroascorbic Acid in Luteinized Rat Ovarian Cells1

Kodaman¹,

Aten²,

Behrman³

1998

Biology of Reproduction

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The corpus luteum is notable for very high levels of ascorbic acid. In luteal cells, ascorbic acid depletion occurs as a result of consumption during radical scavenging, inhibition of ascorbic acid uptake, and stimulation of its secretion. Oxidation of ascorbic acid generates dehydroascorbic acid (DHAA). Although levels of DHAA in blood are much lower than those of ascorbic acid, DHAA serves as the major transportable form of ascorbate for certain cell types. The aim of the present studies was to investigate whether DHAA transport is a potential mechanism for conserving ascorbic acid in the corpus luteum. DHAA uptake by rat luteal cells precultured for 24 h was linear for up to 30 min. Kinetics studies showed that uptake of DHAA was a concentration-dependent and saturable process with an estimated Michaelis constant (Km) of 830 microM and a maximum velocity (Vmax) of 700 pmol/min per 10(6) cells, a rate 50 times that of ascorbate transport. More than 90% of DHAA was reduced to ascorbic acid within 2 h of cellular uptake. DHAA uptake was energy- and microfilament-dependent, as transport was inhibited by 2,4-dinitrophenol (1 mM) and cytochalasin B (10 microM). Menadione (50 microM), an intracellular generator of reactive oxygen species, also markedly reduced DHAA uptake. In contrast to ascorbic acid transport, DHAA uptake was potently inhibited by glucose and phloretin, an inhibitor of glucose transporters, with IC50s of approximately 5 mM and 10 microM, respectively. DHAA uptake appears to occur via an insulin-insensitive transporter, as insulin (10 nM) had no effect on uptake. However, 24-h preincubation with insulin-like growth factor (IGF)-I dose-dependently (10-100 ng/ml) stimulated DHAA uptake; similar concentrations of IGF-II had no effect. The secretion of radioactivity by cells preloaded with radiolabeled DHAA was significantly increased by prostaglandin F2alpha (1 microM). The ability of luteal cells to transport DHAA in a regulated manner may serve to maintain vital levels of ascorbic acid within the corpus luteum.

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Disturbances of Reproduction and Ovarian Changes in the Guinea-Pig in Relation to Vitamin C Deficiency

Cited by 42 publications

References 0 publications

An Investigation into the Possible Relationship between Vitamin C and the Adrenal Cortex of the Guinea Pig

An Investigation into the Possible Relationship between Vitamin C and the Adrenal Cortex of the Guinea Pig

Follicular characteristics and intrafollicular concentrations of nitric oxide and ascorbic acid during ovarian acyclicity in water buffalo (Bubalus bubalis)

Accumulation of Ascorbate by Endocrine-Regulated and Glucose-Sensitive Transport of Dehydroascorbic Acid in Luteinized Rat Ovarian Cells1

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