Disturbance of Antioxidant Enzymes and Purine Metabolism in the Ejaculate of Men Living in Disadvantaged Areas of Kyzylorda Region

Abstract

AIM:Objective of the study was to evaluate the state of the main indicators of antioxidant status and enzymes of purine metabolism in the germ cells of men living in the zone of ecological catastrophe Aral Sea region.METHODS:The criterion for inclusion is the stay of an adult in the Aral Sea area is not less than 5 years, employment in occupations with no more than 2 hazard class. Determination of the activity of adenosine deaminase (ADA) was conducted in semen by the method of Nemechek et al., 1993. Determination of the activity of catalase (CAT) was performed according by the method of Korolyuk et al., 1988.RESULTS:Results of the study indicate a change in the activity of catalase and adenosine deaminase, due to increased levels of oxidative stress and the development of the pathological process.CONCLUSIONS:According to the results of study, it was put the influence of negative factors of the Aral Sea region in men’s sperm of reproductive age gives to disability free-radical processes, that proves changing of ferments of ant oxidative protection Catalase and adenosine deaminase (ADA). This disturbance in men’s sperm of reproductive age leading to increased level of oxidative stress and impaired activity of antioxidant enzymes and purine metabolism, responsible for the abnormal transmembrane and intracellular processes, reflecting the degree of imbalance of enzymes.

An Exploratory Study of the Metabolite Profiling from Pesticides Exposed Workers

“…Kislitskaya et al [ 81 ] analyzed disorders of antioxidant enzymes and purine metabolism in the ejaculate of men exposed to pesticides and air pollutants. The authors claimed that this exposure disturbs the balance of lipid peroxidation and antioxidant activity, activating the formation of free radicals in male germ cells, which leads to increased levels of oxidative stress and decreased purine metabolism.…”

Lakes Drying and Their Adverse Effects on Human Health: A Systematic Review

“…In a study the researchers calculated activity of adenosine deaminase (ADA) and activity of catalase (CAT) indexes by sampling the sperm of those men exposed to Aral Lake drying pollution at least for 5 years. ADA and CAT indexes were changed due to increase in oxidative stress and development of the pathological processes (35). About 39% of the participants had delayed/irregular periods.…”