Distributions of enterococci and human-specific bacteriophages of enterococci in a tropical watershed

Chyerochana, Natcha; Kongprajug, Akechai; Somnark, Pornjira; Kamphaengthong, Pinida Leelapanang; Mongkolsuk, Skorn; Sirikanchana, Kwanrawee

doi:10.1016/j.ijheh.2020.113482

Cited by 11 publications

(5 citation statements)

References 33 publications

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“…Phages are vital for renewal of organic matters, provisions of nutrient supply cycles, serve as genomic reservoirs and drive the bacterial diversity. Several studies has investigated the occurrence of bacteriophages in various water sources [ 2 , 26 , 27 ]. Moreover, bacteriophages detection has been considered as important indicator for fecal pollution [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Novel findings in context of molecular diversity and abundance of bacteriophages in wastewater environments of Riyadh, Saudi Arabia

et al. 2022

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The diversity among bacteriophages depends on different factors like ecology, temperature conditions and genetic pool. Current study focused on isolation, identification and diversity of phages from 34 sewage water samples collected from two different wastewater treatment plants (WWTPs), King Saud University wastewater treatment plants (KSU-WWTP) and Manfoha wastewater treatment plants (MN-WWTP) in Riyadh, Saudi Arabia. Samples were analyzed by PCR and Next Generation Sequencing (NGS). Siphoviridae, Podoviridae and Myoviridae families were detected by family-specific PCR and highest prevalence of Myoviridae 29.40% was found at MN-WWTP followed by 11.76% at KSU-WWTP. Siphoviridae was detected 11.76% at MN-WWTP and 5.88% at KSU-WWTP. Lowest prevalence for Podoviridae family (5.88%) was recorded at MN-WWTP. Significant influence of temporal variations on prevalence of Myoviridae and Siphoviridae was detected in both WWTP and MN-WWTP, respectively. Highest phage prevalence was obtained in August (75%), followed by September (50%). Highest phage prevalence was recorded at a temperature range of 29–33°C. Significant influence of temperature on the prevalence of Myoviridae phages was detected at MN-WWTP. Four bacteriophages with various abundance levels were identified by NGS. Cronobacter virus Esp2949-1 was found first time with highest abundance (4.41%) in wastewater of Riyadh. Bordetella virus BPP1 (4.14%), Dickeya virus Limestone (1.55%) and Ralstonia virus RSA1 (1.04%) were also detected from samples of MN-WWTP. Highest occurrence of Bordetella virus BPP1 (67%) and (33.33%) was recorded at KSU-WWTP and MN-WWTP, respectively. Highest Bordetella virus BPP1 occurrence was recorded in September (50%) followed by August (40%). The findings of study showed new insights of phage diversity from wastewater sources and further large-scale data studies are suggested for comprehensive understanding.

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Section: Discussionmentioning

confidence: 99%

Novel findings in context of molecular diversity and abundance of bacteriophages in wastewater environments of Riyadh, Saudi Arabia

et al. 2022

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“…This ideal sensitivity, high specificity, and abundance compels us to believe that it has great potential. The third type of humanspecific MST markers is the Enterococcus phage, which has been successfully used in many areas to track pollution sources (Purnell et al, 2011;Wangkahad et al, 2017;Chyerochana et al, 2020). In addition to high specificity and sensitivity, the culture technique of Enterococcus phages is relatively simple and economical (Wangkahad et al, 2017).…”

Section: Tracking Human Fecal Sourcesmentioning

confidence: 99%

Bacteriophages in water pollution control: Advantages and limitations

Liu

Sun

et al. 2020

Front. Environ. Sci. Eng.

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“…Nonetheless, the y -intercept of the standard curve is adjusted based on a DNA standard, a so-called calibration control, in each instrumental run . The mixed model for qPCR absolute quantification has been previously used in MST research. ,− …”

Section: Introductionmentioning

confidence: 99%

Effect of Quantitative Polymerase Chain Reaction Data Analysis Using Sample Amplification Efficiency on Microbial Source Tracking Assay Performance and Source Attribution

Kongprajug

Chyerochana

Mongkolsuk

et al. 2020

Environ. Sci. Technol.

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The widely used microbial source tracking (MST) technique, quantitative polymerase chain reaction (qPCR), quantifies host-specific gene abundance in polluted water to identify and prioritize contamination sources. This study characterized the effects of a qPCR data analysis using the sample PCR efficiencies (the LinRegPCR model) on gene abundance and compared them with the standard curve-based method (the mixed model). Five qPCR assays were evaluated: the universal GenBac3, human-specific HF183/BFDrev and CPQ_056, swine-specific Pig-2-Bac, and cattle-specific Bac3qPCR assays. The LinRegPCR model increased the low-copy amplification, especially in the HF183/BFDrev assay, thus lowering the specificity to 0.34. Up to 1.41 log 10 copies/g and 0.41 log 10 copies/100 mL differences were observed for composite fecal and sewage samples (n = 147) by the LinRegPCR approach, corresponding to an 18.2% increase and 6.4% decrease, respectively. Freshwater samples (n = 48) demonstrated a maximum of 1.95 log 10 copies/100 mL difference between the two models. Identical attributing sources by both models were shown in 54.55% of environmental samples; meanwhile, the LinRegPCR approach improved the inability to identify sources by the mixed model in 29.55% of the samples. This study emphasizes the need for a standardized data analysis protocol for qPCR MST assays for interlaboratory consistency and comparability.

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Distributions of enterococci and human-specific bacteriophages of enterococci in a tropical watershed

Cited by 11 publications

References 33 publications

Novel findings in context of molecular diversity and abundance of bacteriophages in wastewater environments of Riyadh, Saudi Arabia

Novel findings in context of molecular diversity and abundance of bacteriophages in wastewater environments of Riyadh, Saudi Arabia

Bacteriophages in water pollution control: Advantages and limitations

Effect of Quantitative Polymerase Chain Reaction Data Analysis Using Sample Amplification Efficiency on Microbial Source Tracking Assay Performance and Source Attribution

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