Distribution patterns of the glucose transporters GLUT4 and GLUT1 in skeletal muscles of rats (Rattus norvegicus), pigs (Sus scrofa), cows (Bos taurus), adult goats, goat kids (Capra hircus), and camels (Camelus dromedarius)

Duehlmeier, R.; Sammet, Katharina; Widdel, Andrea; Engelhardt, W; Wernery, U.; Kinne, Jörg; Sallmann, H.‐P.

doi:10.1016/j.cbpa.2006.10.029

Cited by 29 publications

(29 citation statements)

References 43 publications

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“…These bands are thought to possibly result from posttranslational modifications of GLUT4, such as glycosylation (10,26). Glycosylation has an important role in the maturation of glucose transporters (26).…”

Section: Discussionmentioning

confidence: 99%

Participation of ERα and ERβ in glucose homeostasis in skeletal muscle and white adipose tissue

Barros

Gabbi

Morani

et al. 2009

American Journal of Physiology-Endocrinology and Metabolism

128

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Glucose uptake and homeostasis are regulated mainly by skeletal muscle (SM), white adipose tissue (WAT), pancreas, and the liver. Participation of estradiol in this regulation is still under intense investigation. We have demonstrated that, in SM of male mice, expression of the insulinregulated glucose transporter (GLUT)4 is reduced by estrogen receptor (ER)␤ agonists. In the present study, to investigate the relative contributions of ER␣ and ER␤ in glucose homeostasis, we examined the effects of tamoxifen (Tam) on GLUT4 expression in SM and WAT in wild-type (WT) and ERϪ/Ϫ mice. ER␤Ϫ/Ϫ mice were characterized by fasting hypoglycemia, increased levels of SM GLUT4, pancreatic islet hypertrophy, and a belated rise in plasma insulin in response to a glucose challenge. ER␣Ϫ/Ϫ mice, on the contrary, were hyperglycemic and glucose intolerant, and expression of SM GLUT4 was markedly lower than in WT mice. Tam had no effect on glucose tolerance or insulin sensitivity in WT mice. In ER␣Ϫ/Ϫ mice, Tam increased GLUT4 and improved insulin sensitivity. i.e., it behaved as an ER␤ antagonist in SM but had no effect on WAT. In ER␤Ϫ/Ϫ mice, Tam did not affect GLUT4 in SM but acted as an ER␣ antagonist in WAT, decreasing GLUT4. Thus, in the interplay between ER␣ and ER␤, ER␤-mediated repression of GLUT4 predominates in SM but ER␣-mediated induction of GLUT4 predominates in WAT. This tissue-specific difference in dominance of one ER over the other is reflected in the ratio of the expression of the two receptors. ER␣ predominates in WAT and ER␤ in SM.estrogen receptor-␣; estrogen receptor-␤; glucose transporter 4; tamoxifen FOR SEVERAL DECADES the multiple and sometimes contradictory effects of estrogens on human physiology and disease have puzzled endocrinologists. Even today, the modulatory effects of estrogen on glucose homeostasis are not completely understood. The effects of estrogen on gene regulation are mediated by two sometimes opposing forces, estrogen receptor (ER)␣ and ER␤ (20, 28), in both females and males (19,25). Recent experimental evidence has revealed that estradiol (E 2 ) is an important modulator in tissues previously not considered to be classical estrogen targets.In mice, insulin resistance develops when there is no estrogen (in aromatase-knockout mice) and when ER␣ is inactivated (ER␣Ϫ/Ϫ mice) (5,17,19). In women, a clear relationship between E 2 and glycemia has been observed during the menstrual cycle (32), in gestation (7), in gestational diabetes mellitus, and in polycystic ovarian syndrome (11). In normal males, because of the absence of estrogen cyclicity, the effect of estrogen on glycemia is less obvious, but men with hypoestrogenism or with mutations in the aromatase (13) or ER␣ (31) genes do develop insulin resistance.Insulin is the most important hormone for the maintenance of euglycemia. It regulates carbohydrate metabolism in the liver and glucose uptake in insulin-sensitive tissues, i.e., skeletal muscle (SM) and white adipose tissue (WAT) (12). On binding to its receptors on the cell membrane o...

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Section: Discussionmentioning

confidence: 99%

Participation of ERα and ERβ in glucose homeostasis in skeletal muscle and white adipose tissue

Barros

Gabbi

Morani

et al. 2009

American Journal of Physiology-Endocrinology and Metabolism

128

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“…Camel has unique characteristics enable it to adapt its desert environment such as fluctuation of its body temperature, tolerance of water loss and capability of drinking more water in little time (Schmidt-Nielsen, 1979). The physiology of camel is also unique and interesting (Holler et al, 1989;Shirazi-Beechy et al, 1994;Elmahdi et al, 1997;Zierath et al, 1998;Abdel-Fattah et al, 1999;Kaske et al, 2001;Duehlmeier et al, 2007) so that it needs further biological investigation.…”

Section: Introductionmentioning

confidence: 99%

Molecular Study of Energy Related Mitochondrial Genes in Arabian and Bactrian Camels

Ahmed¹,

El-Shazly²,

Sayed³

2013

American Journal of Biochemistry and Biotechnology

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The single-humped camel, Camelus dromedaries inhabiting Afro-Arabia and the double-humped camel, Camelus bactrianus inhabiting central Asia are the only species in their genus. The present study aimed to amplify and partially sequence the mitochondrial DNA genes encoding for NADH dehydrogenase subunit 1, cytochrome c oxidase subunit 1, ATP synthase subunit 6 (ATP6), cytochrome b and displacement region (d-loop) in the single-humped camel and compare it to their counterparts already sequenced for the doublehumped camel. These energy-related genes showed amino acid substitutions gradually increased according to their locations among macromolecular energy transducers. Both ATP synthase 6 in the central core and cytochrome b in the inner mitochondrial membrane acquired the greatest substitutions of 5 and 7 amino acids, respectively. Cytochrome c oxidase is the terminal complex of the electron transport chain of the inner mitochondrial membrane and it showed no substitutions. These substitutions seemed to be correlated with the energy metabolism in both camel phenotypes. The d-loop showed tandem repeats of six nucleotides at its 3` end with polymorphism between both species without any evidence relates such variation to energy production.

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“…Insulin-insensitive uptake via GLUT1 appears to dominate in ruminating species like cattle, goat and camel, while insulin-sensitive uptake via GLUT4 is prevalent in monogastric species like pig and man (11,16). Thus, especially in monogastric species, skeletal muscle can act as an insulin-regulated storage organ when excess glucose is available (50).…”

mentioning

confidence: 99%

Expression of mRNA for glucose transport proteins in jejunum, liver, kidney and skeletal muscle of pigs

et al. 2009

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Although pigs are adapted to starch-rich diets and have high turnover rates of glucose, very scarce information is available on the molecular basis of glucose transport. Therefore, the present study attempted a systematic screening for the presence of mRNA of glucose transport proteins in main organs of glucose absorption, production and conservation. From the members of the solute carrier family SLC5A (sodium glucose cotransporter), the porcine jejunum was positive for SGLT1 and SGLT3, but also contained detectable levels of SGLT5. Liver contained SGLT1, SGLT5, traces of SGLT3 and, in one of five pigs, SGLT2. Kidney contained SGLT1, SGLT2, SGLT3, SGLT5 and hardly detectable levels of SGLT4. Skeletal muscle showed weak signals for SGLT3 and SGLT5. Screening for members of the SLC2A family (facilitated glucose transporter) in intestine revealed the presence of mRNA for GLUT1, GLUT2, GLUT5, GLUT7 and GLUT8, while GLUT3, GLUT4, GLUT10 and GLUT11 were also detectable. The liver contained GLUT1, GLUT2 and GLUT8 mRNA, while GLUT3, GLUT4, GLUT5, GLUT10 and GLUT11 were poorly detectable. The kidney was positive for GLUT1, GLUT2, GLUT5, GLUT8 and GLUT11, but traces of GLUT3, GLUT4 and GLUT10 could also be detected. Skeletal muscle had the strongest signal for GLUT4, while GLUT1, GLUT3, GLUT5, GLUT8, GLUT10 and GLUT11 showed weak signals. A total of 12 unique partial cDNA sequences were submitted to GenBank. In conclusion, this study provides molecular insight into the organ-specific expression of glucose transporters in pigs and thus sheds light on the way of glucose handling in this omnivorous species.

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Distribution patterns of the glucose transporters GLUT4 and GLUT1 in skeletal muscles of rats (Rattus norvegicus), pigs (Sus scrofa), cows (Bos taurus), adult goats, goat kids (Capra hircus), and camels (Camelus dromedarius)

Cited by 29 publications

References 43 publications

Participation of ERα and ERβ in glucose homeostasis in skeletal muscle and white adipose tissue

Participation of ERα and ERβ in glucose homeostasis in skeletal muscle and white adipose tissue

Molecular Study of Energy Related Mitochondrial Genes in Arabian and Bactrian Camels

Expression of mRNA for glucose transport proteins in jejunum, liver, kidney and skeletal muscle of pigs

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