Distribution Patterns of Ornithine Decarboxylase in Cells and Tissues: Facts, Problems, and Postulates

Schipper, Raymond G.; Verhofstad, A.A.J.

doi:10.1177/002215540205000901

Cited by 37 publications

(27 citation statements)

References 158 publications

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“…Eyfp (green) tagged DdODC was cytoplasmic with predominant intense perinuclear staining (Fig. 6A) showing high expression in vesicle like structure close to the nucleus and is in agreement with majority of earlier studies (Schipper and Verhofstad 2002). Notably, the perinuclear signal was very strong and did not overlap with lysotracker staining (data not shown).…”

Section: Overexpression Of Ddodc Inhibits Cell Proliferation and Leadsupporting

confidence: 92%

Cloning, expression and characterization of the ornithine decarboxylase gene from Dictyostelium discoideum

Kumar¹,

Rafia²,

Saran³

2014

Int. J. Dev. Biol.

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Ornithine decarboxylase (ODC) is a rate limiting enzyme in polyamine synthesis that decarboxylates ornithine to form the diamine putrescine. We report here the isolation, expression and characterization of a homolog of ODC from Dictyostelium discoideum. DdODC is conserved and shows sequence and structural homology with that from human. Both ODC transcript and protein are expressed at all stages of development and show high expression in prestalk/stalk cells. It is cytosolic and predominantly perinuclear in localization. Both overexpression of DdODC and putrescine treatment resulted in inhibition of cell proliferation. KEY WORDS: D. discoideum, ODC expression, putrescinePolyamines are ubiquitously present from bacteria to mammals Tabor 1985, Janne 2004) and regulate cell growth, proliferation, differentiation and cell death. The fast proliferating prokaryotes mostly contain putrescine and spermidine (Tabor and Tabor 1976) while the slow proliferating eukaryotes predominantly have spermidine and spermine (Fillingame 1975). A decrease in intracellular polyamines occurs during aging (Minois et al., 2011) but it still remains unclear whether this is a cause or consequence of aging. Addition of exogenous spermidine can extend lifespan in various animal models through epigenetic modifications, induction of autophagy and suppression of necrosis (Eisenberg et al., 2009).Ornithine decarboxylase (ODC, EC 4.1.1.17) catalyses the first and the rate limiting step of polyamine biosynthesis where Lornithine is converted to putrescine (Heller et al., 1976). It is strictly regulated during development (Heby and Persson 1990) and is involved in synthesis of spermidine and spermine. Decarboxylation of ornithine by ODC is the only pathway for de novo synthesis of polyamines in mammals and higher eukaryotes. The ability to strictly regulate cellular polyamine levels within a very narrow range is critical since extreme levels of polyamines (high or low) result in damaging effects, both on life of the cell and organism as a whole (Pegg and McCann 1982).This study was undertaken to understand the role of putrescine, which is the most abundant polyamine found in Dictyostelium discoideum (Saran 1998 Abbreviations used in this paper: DdODC, Dictyostelium discoideum ODC; ODC, ornithine decarboxylase.grow and divide mitotically but upon starvation and in response to the chemoattractant, cAMP, they enter the developmental phase to ultimately form a fruiting body consisting of dead vacuolated stalk cells and viable spores. Since ODC is responsible for the formation of putrescine we characterized this enzyme. Harris and North (1982) did not find any significant ODC activity during development but Campagne and Lowik (1985) have reported high activity at the slug stage. Our earlier report (Saran 1998) has shown high putrescine levels during slug stage. This is the first report on molecular cloning, expression and characterization of a putative ODC-like sequence from D. discoideum. We found it to be structurally conserved and predomina...

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Section: Overexpression Of Ddodc Inhibits Cell Proliferation and Leadsupporting

confidence: 92%

Cloning, expression and characterization of the ornithine decarboxylase gene from Dictyostelium discoideum

Kumar¹,

Rafia²,

Saran³

2014

Int. J. Dev. Biol.

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“…2D). The predominant presence of SAMDC-eYFP in the cytosol is in agreement with the vast majority of earlier studies, which showed cytoplasmic localization 11 . The role of the SAMDC/polyamine system in the cytoplasm still needs to be elucidated, but numerous studies suggest that polyamines play essential roles in the machinery of protein biosynthesis.…”

Section: Cloning Of Samdc Gene From D Discoideum:-supporting

confidence: 92%

Identification and Cloning of S-Adenosylmethionine Decarboxylase (Samdc) Gene From Dictyostelium Discoideum.

Kumar¹,

Sharma²,

Saran³

2018

IJAR

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“…ArgII is present in the mitochondria of PT, as well as of inner medullary collecting ducts (17) and provides l -ornithine for the synthesis of polyamines (56). The cellular balance of polyamines is necessary for DNA stabilization and replication (57), as well as for the maintenance of PT integrity and function (58).…”

Section: Discussionmentioning

confidence: 99%

l-Citrulline Protects from Kidney Damage in Type 1 Diabetic Mice

et al. 2013

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Rationale: Diabetic nephropathy (DN) is a major cause of end-stage renal disease, associated with endothelial dysfunction. Chronic supplementation of l-arginine (l-arg), the substrate for endothelial nitric oxide synthase (eNOS), failed to improve vascular function. l-Citrulline (l-cit) supplementation not only increases l-arg synthesis, but also inhibits cytosolic arginase I, a competitor of eNOS for the use of l-arg, in the vasculature.Aims: To investigate whether l-cit treatment reduces DN in streptozotocin (STZ)-induced type 1 diabetes (T1D) in mice and rats and to study its effects on arginase II (ArgII) function, the main renal isoform.Methods: STZ-C57BL6 mice received l-cit or vehicle supplemented in the drinking water. For comparative analysis, diabetic ArgII knock out mice and l-cit-treated STZ-rats were evaluated.Results: l-Citrulline exerted protective effects in kidneys of STZ-rats, and markedly reduced urinary albumin excretion, tubulo-interstitial fibrosis, and kidney hypertrophy, observed in untreated diabetic mice. Intriguingly, l-cit treatment was accompanied by a sustained elevation of tubular ArgII at 16 weeks and significantly enhanced plasma levels of the anti-inflammatory cytokine IL-10. Diabetic ArgII knock out mice showed greater blood urea nitrogen levels, hypertrophy, and dilated tubules than diabetic wild type (WT) mice. Despite a marked reduction in collagen deposition in ArgII knock out mice, their albuminuria was not significantly different from diabetic WT animals. l-Cit also restored nitric oxide/reactive oxygen species balance and barrier function in high glucose-treated monolayers of human glomerular endothelial cells. Moreover, l-cit also has the ability to establish an anti-inflammatory profile, characterized by increased IL-10 and reduced IL-1β and IL-12(p70) generation in the human proximal tubular cells.Conclusion: l-Citrulline supplementation established an anti-inflammatory profile and significantly preserved the nephron function during T1D.

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Distribution Patterns of Ornithine Decarboxylase in Cells and Tissues: Facts, Problems, and Postulates

Cited by 37 publications

References 158 publications

Cloning, expression and characterization of the ornithine decarboxylase gene from Dictyostelium discoideum

Cloning, expression and characterization of the ornithine decarboxylase gene from Dictyostelium discoideum

Identification and Cloning of S-Adenosylmethionine Decarboxylase (Samdc) Gene From Dictyostelium Discoideum.

l-Citrulline Protects from Kidney Damage in Type 1 Diabetic Mice

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