Distribution Patterns of Nonhistone Chromosomal Proteins on Polytene Chromosomes: Functional Correlations

Elgin, Sarah C. R.; Amero, Sally Ann; Eissenberg, Joel C.; Fleischmann, Gerhard; Gilmour, David S.; James, Tharappel C.

doi:10.1007/978-1-4613-1037-2_6

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…For instance, it is widely accepted that at the level of polytene chromosome cytology, many proteins and protein complexes map to the interband regions. In particular, this applies to interband-specific proteins Z4 and Chriz (CHRO) [6] – [8] , insulator protein BEAF-32 [9] , [10] , topoisomerase I [11] , “open chromatin”- and transcription-associated proteins, such as actively elongating and initiating RNA polymerase II, P-TEFb, TFIIH, TFIIF, SPT4, SPT5, SPT6, FACT, dMediator, GAF, TRX [12] – [26] , nucleoporins [27] . Likewise, interbands are the home base for many nucleosome-remodeling and histone-modifying enzymes, such as WDS highly conservative protein [28] – [30] and NURF, which increases accessibility of chromatin templates [31] .…”

Section: Introductionmentioning

confidence: 99%

Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster

et al. 2011

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Salivary gland polytene chromosomes demonstrate banding pattern, genetic meaning of which is an enigma for decades. Till now it is not known how to mark the band/interband borders on physical map of DNA and structures of polytene chromosomes are not characterized in molecular and genetic terms. It is not known either similar banding pattern exists in chromosomes of regular diploid mitotically dividing nonpolytene cells. Using the newly developed approach permitting to identify the interband material and localization data of interband-specific proteins from modENCODE and other genome-wide projects, we identify physical limits of bands and interbands in small cytological region 9F13-10B3 of the X chromosome in D. melanogaster, as well as characterize their general molecular features. Our results suggests that the polytene and interphase cell line chromosomes have practically the same patterns of bands and interbands reflecting, probably, the basic principle of interphase chromosome organization. Two types of bands have been described in chromosomes, early and late-replicating, which differ in many aspects of their protein and genetic content. As appeared, origin recognition complexes are located almost totally in the interbands of chromosomes.

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Section: Introductionmentioning

confidence: 99%

Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster

et al. 2011

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“…Fixed tissue was transferred to 45% acetic acid plus 2% formaldehyde for 3 min, a coverslip was added and the glands were squashed (Elgin et al 1988). Slides were frozen on dry ice, the coverslip popped off, and the slides immersed in TBST (10 mM TRIS-HCl, pH 8.0, 150 mM NaCl, 0.05% Tween 20).…”

Section: Immunostaining Of Polytene Chromosomesmentioning

confidence: 99%

Expression and functional analysis of three isoforms of human heterochromatin-associated protein HP1 in Drosophila

Hwang

Worman

et al. 2001

Chromosoma

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Heterochromatin-associated protein 1 (HP1) is a nonhistone chromosomal protein associated with pericentromeric heterochromatin in Drosophila. HP1-like proteins have also been found associated with heterochromatin in human cells. The goal of this study was to determine whether proteins of the structurally conserved human HP1 family exhibit conserved heterochromatin targeting and silencing properties in Drosophila. We established transgenic lines of Drosophila melanogaster expressing each of the three human HP1 proteins, HP1Hsalpha, HP1HSbeta, and HP1Hsgamma, under the Hsp70 heat shock promoter. We show that all three isoforms of human HP1 are stably expressed in Drosophila and are associated with heterochromatin in Drosophila chromosomes. Like Drosophila HP1, all three human HP1 proteins are delocalized by an HP1-POLYCOMB chimeric protein, implying that both human HP1 and Drosophila HP1 interact in a common protein complex, and that at least some aspects of heterochromatin structure are highly conserved throughout the evolution of eukaryotes. Ectopic expression of two of the three human HP1 family proteins significantly enhances heterochromatic silencing in Drosophila.

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“…For instance, it is widely accepted that at the level of polytene chromosome cytology, many proteins and protein complexes map to the interband regions. In particular, this applies to interband-specific proteins Z4 and Chriz (CHRO) [6][7][8], insulator protein BEAF-32 [9,10], topoisomerase I [11], ''open chromatin''-and transcription-associated proteins, such as actively elongating and initiating RNA polymerase II, P-TEFb, TFIIH, TFIIF, SPT4, SPT5, SPT6, FACT, dMediator, GAF, TRX [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26], nucleoporins [27]. Likewise, interbands are the home base for many nucleosome-remodeling and histone-modifying enzymes, such as WDS highly conservative protein [28][29][30] and NURF, which increases accessibility of chromatin templates [31].…”

Section: Introductionmentioning

confidence: 99%

Correction: Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster

Ty¹,

Lv²,

Ov³

et al. 2011

PLoS ONE

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Salivary gland polytene chromosomes demonstrate banding pattern, genetic meaning of which is an enigma for decades. Till now it is not known how to mark the band/interband borders on physical map of DNA and structures of polytene chromosomes are not characterized in molecular and genetic terms. It is not known either similar banding pattern exists in chromosomes of regular diploid mitotically dividing nonpolytene cells. Using the newly developed approach permitting to identify the interband material and localization data of interband-specific proteins from modENCODE and other genomewide projects, we identify physical limits of bands and interbands in small cytological region 9F13-10B3 of the X chromosome in D. melanogaster, as well as characterize their general molecular features. Our results suggests that the polytene and interphase cell line chromosomes have practically the same patterns of bands and interbands reflecting, probably, the basic principle of interphase chromosome organization. Two types of bands have been described in chromosomes, early and late-replicating, which differ in many aspects of their protein and genetic content. As appeared, origin recognition complexes are located almost totally in the interbands of chromosomes.

show abstract

Distribution Patterns of Nonhistone Chromosomal Proteins on Polytene Chromosomes: Functional Correlations

Cited by 7 publications

References 14 publications

Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster

Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster

Expression and functional analysis of three isoforms of human heterochromatin-associated protein HP1 in Drosophila

Correction: Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster

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