Distribution patterns of cannabinoid CB1 receptors in the hippocampus of APPswe/PS1ΔE9 double transgenic mice

Kalifa, Sara; Polston, Eva K.; Allard, Joanne; Manaye, Kebreten F.

doi:10.1016/j.brainres.2010.12.061

Cited by 38 publications

(29 citation statements)

References 54 publications

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“…Finally, sections were mounted on glass slides, cover slipped with Vectashield (Vector Laboratories, Burlingame, CA) and sealed with nail polish. Incubating tissues with cocktails of the primary antibodies and their corresponding antigenic peptides resulted in abolished immunoreactivity and confirmed antibody specificities to their target proteins that were also reported in previous studies (Giuliano et al, 2009; Kalifa et al, 2011; Shahid et al, 2012). …”

Section: Methodssupporting

confidence: 86%

A pivotal role for enhanced brainstem Orexin receptor 1 signaling in the central cannabinoid receptor 1-mediated pressor response in conscious rats

Ibrahim

Abdel‐Rahman

2015

Brain Research

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Orexin receptor 1 (OX 1 R) signaling is implicated in cannabinoid receptor 1 (CB 1 R) modulation of feeding. Further, our studies established the dependence of the central CB 1 R-mediated pressor response on neuronal nitric oxide synthase (nNOS) and extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylation in the RVLM. We tested the novel hypothesis that brainstem orexin-A/OX 1 R signaling plays a pivotal role in the central CB 1 R-mediated pressor response. Our multiple labeling immunofluorescence findings revealed co-localization of CB 1 R, OX 1 R and the peptide orexin-A within the C1 area of the rostral ventrolateral medulla (RVLM). Activation of central CB 1 R following intracisternal (i.c.) WIN55,212-2 (15 µg/rat) in conscious rats caused significant increases in BP and orexin-A level in RVLM neuronal tissue. Additional studies established a causal role for orexin-A in the central CB 1 R-mediated pressor response because (i) selective blockade of central CB 1 R (AM251, 30 µg/rat; i.c.) abrogated WIN55,212-2-evoked increases in RVLM orexin-A level, (ii) the selective OX 1 R antagonist SB-408124 (10 nmol/rat; i.c.) attenuated orexin-A (3 nmol/rat; i.c.) or WIN55,212-2 (15 µg/rat; i.c.)-evoked pressor response while selective CB 1 R blockade (AM251) had no effect on orexin-A (3 nmol/rat; i.c.)-evoked pressor response, (iii) direct CB 1 R activation in the RVLM (WIN55,212-2; 0.1 µg/rat) increased RVLM orexin-A and BP. Finally, SB-408124 attenuated WIN55,212-2-evoked increases in RVLM nNOS and ERK1/2 phosphorylation and BP. Our findings suggest that orexin-A/OX 1 R dependent activation of the RVLM nNOS/ERK1/2 cascade is essential neurochemical mechanism for the central CB 1 R-mediated pressor response in conscious rats.

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Section: Methodssupporting

confidence: 86%

A pivotal role for enhanced brainstem Orexin receptor 1 signaling in the central cannabinoid receptor 1-mediated pressor response in conscious rats

Ibrahim

Abdel‐Rahman

2015

Brain Research

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“…Huntington's disease [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17]. The neuroprotective effects of cannabinoids are mediated through CB1 receptors, located in neurons, which modulate neuronal function, and CB2 receptors, mostly located in microglia, which play an important role in neuroinflammation.…”

Section: Mj Casarejos Et Al / Pk −/− /Tau Vlw Mice and Phytocannabmentioning

confidence: 99%

Natural Cannabinoids Improve Dopamine Neurotransmission and Tau and Amyloid Pathology in a Mouse Model of Tauopathy

Casarejos

Perucho

Gómez

et al. 2013

JAD

107

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Abstract. Cannabinoids are neuroprotective in models of neurodegenerative dementias. Their effects are mostly mediated through CB1 and CB2 receptor-dependent modulation of excitotoxicity, inflammation, oxidative stress, and other processes. We tested the effects of Sativex ® , a mixture of 9 -tetrahydrocannabinol and cannabidiol, acting on both CB1 and CB2 receptors, in parkin-null, human tau overexpressing (PK −/− /Tau VLW ) mice, a model of complex frontotemporal dementia, parkinsonism, and lower motor neuron disease. The animals received Sativex ® , 4.63 mg/kg, ip, daily, for one month, at six months of age, at the onset of the clinical symptoms. We evaluated the effects of Sativex ® on behavior, dopamine neurotransmission, glial activation, redox state, mitochondrial activity, and deposition of abnormal proteins. PK −/− /Tau VLW mice developed the neurological deficits, but those treated with Sativex ® showed less abnormal behaviors related to stress, less auto and hetero-aggression, and less stereotypy. Sativex ® significantly reduced the intraneuronal, MAO-related free radicals produced during dopamine metabolism in the limbic system. Sativex ® also decreased gliosis in cortex and hippocampus, increased the ratio reduced/oxidized glutathione in the limbic system, reduced the levels of iNOS, and increased those of complex IV in the cerebral cortex. With regard to tau and amyloid pathology, Sativex ® reduced the deposition of both in the hippocampus and cerebral cortex of PK −/− /Tau VLW mice and increased autophagy. Sativex ® , even after a short administration in animals with present behavioral and pathological abnormalities, improves the phenotype, the oxidative stress, and the deposition of proteins in PK −/− /Tau VLW mice, a model of complex neurodegenerative disorders.

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“…In the present study, the binding levels of [ 3 H] SR141716A in hippocampus were higher than most of other regions, indicating the strong influence of CB1 receptors on hippocampus belonging to the limbic system [22]. CB1 receptors are believed as a critical modulator for stress responses and found to decrease in the hippocampus of rats exposed to chronic stress accompanied by impairment of reversal learning and spatial memory, which is reversed by CB1 receptor agonist [21].…”

Section: Simvastatin Attenuated the Declined Cb1 Receptor Expressionsmentioning

confidence: 40%

Reversal effect of simvastatin on the decrease in cannabinoid receptor 1 density in 6-hydroxydopamine lesioned rat brains

et al. 2016

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Distribution patterns of cannabinoid CB1 receptors in the hippocampus of APPswe/PS1ΔE9 double transgenic mice

Cited by 38 publications

References 54 publications

A pivotal role for enhanced brainstem Orexin receptor 1 signaling in the central cannabinoid receptor 1-mediated pressor response in conscious rats

A pivotal role for enhanced brainstem Orexin receptor 1 signaling in the central cannabinoid receptor 1-mediated pressor response in conscious rats

Natural Cannabinoids Improve Dopamine Neurotransmission and Tau and Amyloid Pathology in a Mouse Model of Tauopathy

Reversal effect of simvastatin on the decrease in cannabinoid receptor 1 density in 6-hydroxydopamine lesioned rat brains

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