Distribution pattern of nicotinic acetylcholine receptors in developing cerebellum of rat neonates born of diabetic mothers

Acetylcholine Neurons Become Cholinergic during Three Time Windows in the Developing Mouse Brain

“…ACh neurons and nAChRs can be detected from an early time point in the embryonic brain but the findings vary based on the experimental conditions ( Cimino, 1995 ; Jaarsma et al, 1997 ; Zhang, 1998 ; Abreu-Villaca et al, 2011 ; Zhang et al, 2016 ; Broide et al, 2019 ; Abbasi et al, 2020 ; Alzu’bi et al, 2020 ; Colangelo et al, 2022 ; Saad et al, 2022 ). While it has been shown that hindbrain CN neurons are born approximately at E9, basal forebrain ACh neuron between E10 and E13.5, and neurons in the habenula between E14 and E18, the date of birth does not explain when ACh neurons become cholinergic ( Finlay and Darlington, 1995 ; Allaway et al, 2020 ; Ananth et al, 2023 ; Funato et al, 2000 ).…”

“…Despite recent breakthroughs, such as reconstruction of individual ACh neuron projection fields, the generation of whole-brain atlases of ACh neurons or input/output mapping to/from the basal forebrain ACh neurons, and RNA expression patterns in the developed brain, very little progress has been made regarding a comprehensive assessment of developmental changes in ACh neurons ( Oh et al, 2014 ; Wu et al, 2014 ; Gielow and Zaborszky, 2017 ; Li et al, 2018 ; Cizeron, 2020 ; Wang et al, 2020 ; Tomas-Roca et al, 2022 ; Gamage et al, 2023 ). While there is a trove of literature about nAChR mRNA or protein level changes in many brain regions in the pre- or postnatal rodent brain, there is little data about how onset of cholinergic signaling overlaps with the nAChR expression patterns in these brain regions ( Cimino, 1995 ; Jaarsma et al, 1997 ; Zhang, 1998 ; Abreu-Villaca et al, 2011 ; Zhang et al, 2016 ; Broide et al, 2019 ; Abbasi et al, 2020 ; Alzu’bi et al, 2020 ; Colangelo et al, 2022 ; Saad et al, 2022 ).…”