Distribution of Wolbachia infection in Chorthippus parallelus populations within and beyond a Pyrenean hybrid zone

Zabal‐Aguirre, Mario; Arroyo, F.; Bella, José L.

doi:10.1038/hdy.2009.106

Cited by 28 publications

(53 citation statements)

References 55 publications

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“…S6). In the bat fly, the louse fly, the grasshopper, and the termites, the F Wolbachia infections are not fixed in the host populations, suggesting facultative nature of these host-symbiont associations (32)(33)(34)(35). These observations suggest that (i) the bedbug-wCle nutritional mutualism evolved independently of the nematodeWolbachia mutualism within the Wolbachia F supergroup, (ii) the origin of the bedbug-wCle mutualistic association is more recent than the origin of the nematode-Wolbachia mutualistic association, (iii) plausibly, an ancestor of the cimicid bugs acquired wCle from an unrelated arthropod host, (iv) in the donor arthropod, wCle was likely a facultative endosymbiotic associate, and (v) the establishment of the bedbug-wCle association presumably entailed an evolutionary transition from facultative symbiosis to obligate nutritional mutualism for the symbiont side.…”

Section: Conserved Biotin Operon Inserted In the Wolbachia Genomementioning

confidence: 99%

Evolutionary origin of insect– Wolbachia nutritional mutualism

Nikoh

Hosokawa

Moriyama

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

364

407

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Obligate insect-bacterium nutritional mutualism is among the most sophisticated forms of symbiosis, wherein the host and the symbiont are integrated into a coherent biological entity and unable to survive without the partnership. Originally, however, such obligate symbiotic bacteria must have been derived from free-living bacteria. How highly specialized obligate mutualisms have arisen from less specialized associations is of interest. Here we address this evolutionary issue by focusing on an exceptional insect-Wolbachia nutritional mutualism. Although Wolbachia endosymbionts are ubiquitously found in diverse insects and generally regarded as facultative/parasitic associates for their insect hosts, a Wolbachia strain associated with the bedbug Cimex lectularius, designated as wCle, was shown to be essential for host's growth and reproduction via provisioning of B vitamins. We determined the 1,250,060-bp genome of wCle, which was generally similar to the genomes of insect-associated facultative Wolbachia strains, except for the presence of an operon encoding the complete biotin synthetic pathway that was acquired via lateral gene transfer presumably from a coinfecting endosymbiont Cardinium or Rickettsia. Nutritional and physiological experiments, in which wCle-infected and wCle-cured bedbugs of the same genetic background were fed on B-vitaminmanipulated blood meals via an artificial feeding system, demonstrated that wCle certainly synthesizes biotin, and the wCleprovisioned biotin significantly contributes to the host fitness. These findings strongly suggest that acquisition of a single gene cluster consisting of biotin synthesis genes underlies the bedbug-Wolbachia nutritional mutualism, uncovering an evolutionary transition from facultative symbiosis to obligate mutualism facilitated by lateral gene transfer in an endosymbiont lineage.

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Section: Conserved Biotin Operon Inserted In the Wolbachia Genomementioning

confidence: 99%

Evolutionary origin of insect– Wolbachia nutritional mutualism

Nikoh

Hosokawa

Moriyama

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

364

407

View full text Add to dashboard Cite

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“…In total, 103 individuals from the hybrid population of Portalet were analysed. This population was selected because it has individuals harboring the distinct X morphs [Serrano et al, 1996] and has recently been characterized for the presence of Wolbachia [Zabal-Aguirre et al, 2010]. Wolbachia infection was analysed by PCR, resulting in 73 samples that were infected and 30 that were uninfected.…”

Section: Chromosome Markersmentioning

confidence: 99%

“…In a previous paper [Martínez et al, 2009], we showed that Wolbachia is widely spread in different tissues of the infected individuals of C. parallelus, featuring high bacterial density in gonads of both sexes. Additionally, at least 2 different Wolbachia strains with different geographically-dependent infection patterns have been found in the C. parallelus populations [Zabal-Aguirre et al, 2010], but it is more remarkable that in these populations Wolbachia produces significant unidirectional CI Zabal-Aguirre et al, in preparation].…”

mentioning

confidence: 99%

Wolbachia Induced Cytogenetical Effects as Evidenced in Chorthippus parallelus (Orthoptera)

Sarasa

Bernal²,

Fernández-Calvı́n³

et al. 2012

Cytogenet Genome Res

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The cytoplasmic incompatibility induced by the bacterial endosymbiont Wolbachia is attributed to chromatin modification in the sperm of infected individuals and is only ‘rescued’ by infected females after fertilization. Chorthippus parallelus is a grasshopper with 2 subspecies that form a hybrid zone in the Pyrenees in which this Wolbachia-generated cytoplasmic incompatibility has recently been described. The analysis of certain cytogenetic traits (sex chromosome-linked heterochromatic bands, nucleolar organizing region expression, spermatid size and morphology, and number of chiasmata formed) in pure and hybrid Chorthippus parallelus that are infected and not infected by this bacterium indicates that the infection affects some of these traits and, in the case of the spermatids, reveals a synergism between the infection and the hybrid condition. These results are interpreted as being secondary effects of the chromatin modification induced by Wolbachia which thereby support this model of modification/rescue. The possible effects of these cytogenetic variations on affected individuals are also considered.

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“…Wolbachia влияет на репродуктивную биологию макросимбионтов, в частности может как увеличивать, так и сокращать репродуктивный потенциал особей в попу-ляции. На популяционном уровне присутствие Wolbachia может оказывать влияние на генетическое разнообразие вида (Werren et al, 2008;Zabal-Aguirre et al, 2010).…”

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