Distribution of vascular endothelial growth factor receptor-3/Flt4 mRNA in adult rat central nervous system

Hou, Yun; Shin, Yoo-Jin; Han, Esther Jiwon; Choi, Jeong-Sun; Park, Jang-Mi; Cha, Jung-Ho; Choi, Jinhyeok; Lee, Mun‐Yong

doi:10.1016/j.jchemneu.2011.06.001

Cited by 15 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition to their specific function in lymphangiogenesis, VEGF-C and VEGFR-3 have also been detected in the mouse brain from embryonic day 15.5, particularly in the olfactory bulb and the optic nerve region, acting as a selective growth factor for neural progenitor cells (Le Bras et al, 2006). Interestingly, our group showed that VEGF-C and VEG-FR-3 are expressed in the cortical and hippocampal neurons in the adult rat brain and that their expression is regulated in response to cerebral ischemia (Shin et al, 2008;Hou et al, 2011). In addition, our previous studies showed that VEGF-C mRNA and VEGFR-3 protein expressions were induced in macrophages and reactive astrocytes in the rat brain after middle cerebral artery occlusion-and 4-vessel occlusion-induced lethal ischemia (Shin et al, 2008(Shin et al, , 2010b.…”

Section: Discussionmentioning

confidence: 86%

Ischemic tolerance is associated with VEGF-C and VEGFR-3 signaling in the mouse hippocampus

Bhuiyan¹,

Kim²,

Hwang³

et al. 2015

Neuroscience

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 86%

Ischemic tolerance is associated with VEGF-C and VEGFR-3 signaling in the mouse hippocampus

Bhuiyan¹,

Kim²,

Hwang³

et al. 2015

Neuroscience

View full text Add to dashboard Cite

“…VEGFR3, a receptor for lymphogenesis, is expressed by neural stem cells and is directly required for neurogenesis (Calvo et al ., ). VEGFR3 mRNA is expressed abundantly in tanycytes of the ME of adults (Hou et al ., ), although the present study showed that the expression of VEGFR2 was not observed at tanycytes of the ependymal zone. The present study showed that SU11248 significantly decreased the number of BrdU‐positive PPCs in the ME by 86.4% as compared with the vehicle.…”

Section: Discussionmentioning

confidence: 97%

VEGF‐dependent continuous angiogenesis in the median eminence of adult mice

Morita

Ukai

Miyata

2012

Eur J of Neuroscience

View full text Add to dashboard Cite

Brain vasculature forms the blood-brain barrier (BBB) that restricts the movement of molecules between the brain and blood, but the capillary of the median eminence (ME) lacks the BBB for secretion of adenohypophysial hormone-releasing peptides. In the present study, we aimed to elucidate whether continuous angiogenesis occurs in the ME of adult mice. By using a mitotic marker, bromodeoxyuridine (BrdU), we demonstrated that new endothelial cells were born continuously in the ME of adults. Prominent expression of NG2, platelet-derived growth factor receptor B (PDGFRB), and delta-like ligand 4 was observed at pericytes of adults, although the expression of these angiogenesis-associated proteins has been shown to be at low or trace levels in adult mature capillary. In addition, vascular endothelial growth factor (VEGF), a key regulator of angiogenesis, was expressed highly in the nervous parenchyma of the ME. Expression of VEGF receptor 2 (VEGFR2) was observed at endothelial cells in the external zone and at somatodendrites in the internal zone. Finally, a VEGFR- and PDGFR-associated tyrosine kinase inhibitor, SU11248, significantly decreased the number of BrdU-positive proliferating endothelial cells and parenchyma cells. In conclusion, the present study demonstrates VEGF-dependent continuous angiogenesis in the ME of adult mouse brains under normal conditions, which provides new insight into our understanding of neurosecretion in the ME.

show abstract

“…It is not clear whether the growth factors from PRP acted on the brain or on the spinal cord. It is difficult to clarify this point because of the fact that the receptors of growth factors that enhanced axon growth, including VEGF and IGF-1, have been reported to be present in both the brain cortex and the spinal cord [3,14,15]. In previous studies, although the administration of neurotropic factors markedly enhanced axon growth in cocultures from postnatal day 3 (P3) rats, only a slight enhancement in axon growth has been observed in cocultures from postnatal day 7 (P7) rats.…”

Section: Discussionmentioning

confidence: 99%

Human platelet-rich plasma promotes axon growth in brain–spinal cord coculture

et al. 2012

View full text Add to dashboard Cite

Platelet-rich plasma (PRP) contains several growth factors, including platelet-derived growth factor (PDGF), transforming growth factor-β1 (TGF-β1), insulin-like growth factor-1 (IGF-1), and vascular endothelial growth factor (VEGF), that are associated with repair processes after central nervous system injury. Although PRP have been applied to some regenerative therapies, the regeneration effects of PRP on spinal cord injury have not been reported. This study applied a rat organ coculture system to examine the ability of PRP to enhance axonal growth in spinal cord tissues and to identify the growth factors in PRP that contribute to the regulation of axon growth. PRP from human peripheral blood was added to organ cocultures. Furthermore, neutralizing antibodies against PDGF-AB, TGF-β1, IGF-1, or VEGF were added to the cocultures with PRP. Axon growth from the brain cortex into the spinal cord was assessed quantitatively using anterograde axon tracing with DiI. Addition of PRP to the cocultures promoted axon growth, and the axon growth was significantly suppressed by the addition of neutralizing antibodies against IGF-1 and VEGF, but not PDGF-AB. In contrast, axon growth was promoted significantly by the addition of neutralizing antibodies against TGF-β1. These findings indicate that PRP promotes axon growth in spinal cord tissues through mechanisms associated with IGF-1 and VEGF, and that TGF-β1 in PRP exerts negative effects on axon growth.

show abstract

Distribution of vascular endothelial growth factor receptor-3/Flt4 mRNA in adult rat central nervous system

Cited by 15 publications

References 26 publications

Ischemic tolerance is associated with VEGF-C and VEGFR-3 signaling in the mouse hippocampus

Ischemic tolerance is associated with VEGF-C and VEGFR-3 signaling in the mouse hippocampus

VEGF‐dependent continuous angiogenesis in the median eminence of adult mice

Human platelet-rich plasma promotes axon growth in brain–spinal cord coculture

Contact Info

Product

Resources

About