Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection

Tarchouna, Mouna; Ferjani, Asma; Ben-Selma, Walid; Boukadida, Jalel

doi:10.1016/j.ijid.2013.01.025

Cited by 126 publications

(150 citation statements)

References 16 publications

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“…Additionally, all investigated virulence genes were more prevalent in pathogenic isolates than in commensal isolates, consistent with previous studies [2,31]. The fimH gene (81.6%) was the most frequent virulence gene, particularly as it appeared in 95.8% of uropathogenic isolates, which is in accordance with a previous study that reported that the fimH gene played an important role in the initiation of colonization and infection, particularly in urinary tract infections [24,32]. papG allele I (0.6%) and papG allele II (3.4%) were rarely found compared with the papG allele III (23.6%), indicating that they have little importance in the pathogenesis of E. coli.…”

Section: Discussionsupporting

confidence: 91%

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Liu

et al. 2017

J Infect Dev Ctries

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Introduction: Escherichia coli is not only a commensal organism in humans and animals, but also a causative agent of diarrhea and extraintestinal infections. Information about the relationship between population structure, virulence gene profiles, and fluoroquinolone resistance of E. coli in dogs and cats in China is limited. Methodology: A total of 174 pathogenic and commensal E. coli isolates were evaluated in terms of phylogenetic group, virulence gene profile, sequence types (STs), and fluoroquinolone susceptibility. Results: A total of 46.6% of isolates belonged to phylogenetic group B2. Isolates displayed high resistance to tetracycline (82.2%), amoxicillin/clavulanic acid (73.6%), gentamicin (62.1%), and enrofloxacin (60.9%). fimH (81.6%) was the most prevalent virulence gene, and 83.9% of isolates contained one or more investigated virulence genes. The majority of the investigated virulence genes were more prevalent in fluoroquinolone-susceptible isolates and pathogenic isolates. Multilocus sequence typing (MLST) showed that E. coli isolates analyzed were assigned to 65 STs. Among of them, pathogenic-resistant and pathogenic-susceptible isolates had 44 and 10 STs, respectively, while there were 8 and 3 STs in the commensal resistant and susceptible isolates, respectively. Conclusions: Phylogenetic group B2 was the dominant group, accounting for 46.6% of the isolates. Pathogenic isolates and fluoroquinolonesusceptible isolates possessed more virulence genes. Pathogenic isolates and fluoroquinolone-resistant isolates exhibited high population diversity, and pandemic clone ST131 appeared in 9.8% of isolates.

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Section: Discussionsupporting

confidence: 91%

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Liu

et al. 2017

J Infect Dev Ctries

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“…A subset of E. coli is capable of causing enteric/diarrheal diseases and a different cluster causes extra-intestinal infections, including urinary tract infection (UTI) (1). Uropathogenic E. coli (UPEC), a gram negative bacillus, is the main etiologic agent and predominant microorganism causing UTIs (in 50-80z) (2)(3)(4). UTI is the most common bacterial infectious disease encountered in clinical practice, accounting for considerable morbidity rates and high medical costs (4).…”

Section: Introductionmentioning

confidence: 99%

“…Uropathogenic E. coli (UPEC), a gram negative bacillus, is the main etiologic agent and predominant microorganism causing UTIs (in 50-80z) (2)(3)(4). UTI is the most common bacterial infectious disease encountered in clinical practice, accounting for considerable morbidity rates and high medical costs (4). UTIs are one of the most prevalent and significant community-acquired and nosocomial infections, with 150 million cases of this extraintestinal infection occurring annually worldwide (5,6).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology of Multidrug Resistant Uropathogenic <i>Escherichia coli</i> in Iran: a Systematic Review and Meta-Analysis

et al. 2017

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“…These results agree with published reports, which emphasize the predominance of fimbriae type 1 among the UPEC strains (Jalali et. al., 2015;Tarchouna et al, 2013;Usein et al, 2001). One important virulence factor of E. coli causing UTI is fimbriae-mediated adherence.…”

Section: Discussionmentioning

confidence: 99%

Genotypic detection of the virulence factors of uropathogenic Escherichia coli isolated from diarrheic and urinary tract infected patients in Khartoum State, Sudan

Husam¹,

Altayb²,

Mogahid³

et al. 2018

Afr. J. Microbiol. Res.

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This study aimed to identify some important virulence factors, including pap, fim, sfa, aer and hly genes, typical of uropathogenic Escherichia coli (UPEC) in isolates collected from diarrheic and urinary tract infected patients in Khartoum State by multiplex polymerase chain reaction (PCR) assay. A total of 100 clinical specimens (50 urine and 50 diarrhea) were collected. Samples were cultured and identified by conventional method. Most study population were females 57/100 (57%); 42 suffering from urinary tract infections (UTIs) and 15 from diarrhea, while males were 43/100 (43%); 8 suffering from UTIs and 35 from diarrhea. Among enrolled subjects, 83 were positive for one or more uropathogenic E. coli virulent genes, while 17 isolates were negative for all genes. The results of multiplex PCR revealed that thirty two (n=32) diarrheal samples and fourteen (n=14) urine samples were aer positive. Thirty three (n=33) urine samples and eight (n=8) diarrheal samples appeared as fim positive. The genes pap and hly were found in 24 and 14 urine samples, respectively and in 9 and 3 diarrheal samples, respectively, while sfa gene was detected only in 15 urine specimens. The study concluded that fim gene was highly prevalent among UTI patients while aer gene was highly prevalent among diarrhea patients.

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Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection

Abstract: Our study showed that investigation of the bacterial pathogenicity associated with UTI may contribute to a better medical intervention.

Cited by 126 publications

References 16 publications

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Epidemiology of Multidrug Resistant Uropathogenic <i>Escherichia coli</i> in Iran: a Systematic Review and Meta-Analysis

Genotypic detection of the virulence factors of uropathogenic Escherichia coli isolated from diarrheic and urinary tract infected patients in Khartoum State, Sudan

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