Distribution of type A and type B EBV in normal individuals and patients with head and neck carcinomas in Taiwan

Shu, Ching‐Fong; Chang, Ya-Ru; Liang, Chunshui; Liu, S.T.; Lin, Chung-Chi; Chang, Peter Mu Hsin

doi:10.1016/0166-0934(92)90175-d

Cited by 12 publications

(6 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This study using PCR on fresh-frozen samples from NPCs showed that EBV type A is predominant and del-LMP-1 is common in the Japanese population, findings which agree with data acquired from the same sort of specimens collected in Southeast Asia and North Africa [19] , [20] , [52] , [53] . The opposite has been observed in samples from European EBV-associated NPC patients and healthy donors, where wild-type LMP-1 isolates are more prominent than del-LMP-1 variants [54] .…”

Section: Discussionsupporting

confidence: 88%

Epstein-Barr Virus and Human Papillomavirus Infections and Genotype Distribution in Head and Neck Cancers

et al. 2014

View full text Add to dashboard Cite

ObjectiveTo investigate the prevalence, genotypes, and prognostic values of Epstein-Barr virus (EBV) and human papillomavirus (HPV) infections in Japanese patients with different types of head and neck cancer (HNC).Methods and MaterialsHPV and EBV DNA, EBV genotypes and LMP-1 variants, and HPV mRNA expression were detected by PCR from fresh-frozen HNC samples. HPV genotypes were determined by direct sequencing, and EBV encoded RNA (EBER) was examined by in situ hybridization.ResultsOf the 209 HNC patients, 63 (30.1%) had HPV infection, and HPV-16 was the most common subtype (86.9%). HPV E6/E7 mRNA expression was found in 23 of 60 (38.3%) HPV DNA-positive cases detected. The site of highest prevalence of HPV was the oropharynx (45.9%). Among 146 (69.9%) HNCs in which EBV DNA was identified, 107 (73.3%) and 27 (18.5%) contained types A and B, respectively, and 124 (84.9%) showed the existence of del-LMP-1. However, only 13 (6.2%) HNCs were positive for EBER, 12 (92.3%) of which derived from the nasopharynx. Co-infection of HPV and EBER was found in only 1.0% of HNCs and 10.0% of NPCs. Kaplan-Meier survival analysis showed significantly better disease-specific and overall survival in the HPV DNA+/mRNA+ oropharyngeal squamous cell carcinoma (OPC) patients than in the other OPC patients (P = 0.027 and 0.017, respectively). Multivariate analysis showed that stage T1–3 (P = 0.002) and HPV mRNA-positive status (P = 0.061) independently predicted better disease-specific survival. No significant difference in disease-specific survival was found between the EBER-positive and -negative NPC patients (P = 0.155).ConclusionsOur findings indicate that co-infection with HPV and EBV is rare in HNC. Oropharyngeal SCC with active HPV infection was related to a highly favorable outcome, while EBV status was not prognostic in the NPC cohort.

show abstract

Section: Discussionsupporting

confidence: 88%

Epstein-Barr Virus and Human Papillomavirus Infections and Genotype Distribution in Head and Neck Cancers

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The close association of EBV and NPC has been demonstrated for decades. [5][6][7][8][9][10][11][12][13][14][15][16][17] In the 1970s, EBV genome was demonstrated in the undifferentiated type of NPC only. 12,13 Recently, by use of more sensitive methods such as PCR and EBER in situ hybridization, EBV has been demonstrated in almost every NPC tumor.…”

Section: Discussionmentioning

confidence: 99%

“…The association of Epstein-Barr virus (EBV) and NPC has been demonstrated for decades. [5][6][7][8][9][10][11] EBV harboring in the NPC cells was restricted to undifferentiated and nonkeratinizing carcinomas in some reports. 12,13 After wide application of polymerase chain reaction (PCR) and EBVencoded ribonucleic acid (EBER) in situ hybridization, it has now been demonstrated that EBV is harbored in almost every NPC tumor irrespective of histologic differentiation or geographic distribution.…”

mentioning

confidence: 99%

Nasopharyngeal swab and pcr for the screening of nasopharyngeal carcinoma in the endemic area: A good supplement to the serologic screening

Sheen

Chang

et al. 1998

Head Neck

View full text Add to dashboard Cite

Background Nasopharyngeal carcinoma (NPC) is a common head and neck cancer in Taiwan. Early detection is the best way to improve survival for this disease. A prospective study was designed to assess the feasibility of a new screening method for NPC by use of polymerase chain reaction (PCR) based on the close association of NPC and Epstein‐Barr virus (EBV). Methods One hundred thirty‐three different tissues from nasopharynx, nose, and sinus were investigated by use of PCR to check for the presence of EBV genome. Subsequently, from April 1996 to March 1997, 55 patients were enrolled in a prospective screening study. All patients underwent nasopharyngeal swabs before biopsy. Polymerase chain reaction detection of EBV genome was conducted on swab samples. Anti‐EBV viral capsid antigen (VCA) in IgA and IgG class were checked at the same visit. Results The EBV genome was present in 91.4% (85/93) of NPC tissues and in 25.0% (10/40) of non‐NPC tissues (p< .001, χ2 test). Of the 55 swabs, 49 (89.1%) specimens obtained enough cells for PCR examination. Thirty of these 49 patients were pathologically proved NPC. The presence of EBV were 86.7% (26/30) in NPC group and 42.1% (8/19) in non‐NPC group. The sensitivity and specificity were 86.7% and 57.9%, respectively, which were similar to those of serologic method (87.5% and 43.5%) when the cut‐off point was set at anti‐VCA IgG ≥ 160 and IgA ≥ 10. Combining both methods elevates the specificity to 84.2%. Conclusions The sensitivity of this PCR screening method is similar to that of the serologic method. Combining both methods can greatly increase the specificity, indicating this new method is a good supplement to the serologic screening of this endemic disease. © 1998 John Wiley & Sons, Inc. Head Neck 20: 732–738, 1998.

show abstract

“…Although the epidemiological data remain sparse, the geographic distribution of both virus types seems to vary extensively across the world. Type 1 viruses seem to largely dominate in Asia and in Europe, but both type 1 and type 2 viruses are found at more comparable rates in Africa, New Guinea, Australia, the USA, and more specifically Alaska, as well as in transplant recipients and in HIV-positive individuals (Aitken et al 1994;Young et al 1987;Sculley et al 1988;Sixbey et al 1989;Kunimoto et al 1992;Shu et al 1992;Kyaw et al 1992;Apolloni and Sculley 1994). In the latter group, EBV type 2 is transmitted by sexual route in individuals with multiple partners, some of which of non-European origin.…”

Section: Ebv Type 1 and Type 2 Two Strains With Different Biologicalmentioning

confidence: 96%

Epstein–Barr Virus: From the Detection of Sequence Polymorphisms to the Recognition of Viral Types

Feederle

Klinke

Kutikhin

et al. 2015

Current Topics in Microbiology and Immunology

View full text Add to dashboard Cite

The Epstein-Barr virus is etiologically linked with the development of benign and malignant diseases, characterized by their diversity and a heterogeneous geographic distribution across the world. The virus possesses a 170-kb-large genome that encodes for multiple proteins and non-coding RNAs. Early on there have been numerous attempts to link particular diseases with particular EBV strains, or at least with viral genetic polymorphisms. This has given rise to a wealth of information whose value has been difficult to evaluate for at least four reasons. First, most studies have looked only at one particular gene and missed the global picture. Second, they usually have not studied sufficient numbers of diseased and control cases to reach robust statistical significance. Third, the functional significance of most polymorphisms has remained unclear, although there are exceptions such as the 30-bp deletion in LMP1. Fourth, different biological properties of the virus do not necessarily equate with a different pathogenicity. This was best illustrated by the type 1 and type 2 viruses that markedly differ in terms of their transformation abilities, yet do not seem to cause different diseases. Reciprocally, environmental and genetic factors in the host are likely to influence the outcome of infections with the same virus type. However, with recent developments in recombinant virus technology and in the availability of high throughput sequencing, the tide is now turning. The availability of 23 complete or nearly complete genomes has led to the recognition of viral subtypes, some of which possess nearly identical genotypes. Furthermore, there is growing evidence that some genetic polymorphisms among EBV strains markedly influence the biological and clinical behavior of the virus. Some virus strains are endowed with biological properties that explain crucial clinical features of patients with EBV-associated diseases. Although we now have a better overview of the genetic diversity within EBV genomes, it has also become clear that defining phenotypic traits evinced by cells infected by different viruses usually result from the combination of multiple polymorphisms that will be difficult to identify in their entirety. However, the steadily increasing number of sequenced EBV genomes and cloned EBV BACS from diseased and healthy patients will facilitate the identification of the key polymorphisms that condition the biological and clinical behavior of the viruses. This will allow the development of preventative and therapeutic approaches against highly pathogenic viral strains.

show abstract

Distribution of type A and type B EBV in normal individuals and patients with head and neck carcinomas in Taiwan

Cited by 12 publications

References 12 publications

Epstein-Barr Virus and Human Papillomavirus Infections and Genotype Distribution in Head and Neck Cancers

Epstein-Barr Virus and Human Papillomavirus Infections and Genotype Distribution in Head and Neck Cancers

Nasopharyngeal swab and pcr for the screening of nasopharyngeal carcinoma in the endemic area: A good supplement to the serologic screening

Epstein–Barr Virus: From the Detection of Sequence Polymorphisms to the Recognition of Viral Types

Contact Info

Product

Resources

About