Distribution of transient receptor potential vanilloid 1 channel-expressing nerve fibers in mouse rectal and colonic enteric nervous system: relationship to peptidergic and nitrergic neurons

Matsumoto, Kenjiro; Hosoya, Takuji; Tashima, Kimihito; Namiki, Takao; Murayama, Toshihiko; Horie, Syunji

doi:10.1016/j.neuroscience.2010.10.024

Cited by 58 publications

(49 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies, however, were unable to confirm this intrinsic expression in the rodent GI tract (Patterson et al 2003;Ward et al 2003;Schicho et al 2004;Suckow and Caudle 2008;Matsumoto et al 2011) or the human colon (Yiangou et al 2001). Using RT-PCR, we have shown the presence of TRPV1 mRNA in the mouse GI tract, which suggests intrinsic expression of TRPV1, although others demonstrated that TRPV1 mRNA disappears from the rat GI tract after vagotomy and celiac ganglionectomy (Schicho et al 2004).…”

Section: Discussionmentioning

confidence: 54%

“…pigs (Patterson et al 2003;Ward et al 2003;Kadowaki et al 2004;Schicho et al 2004;Matsumoto et al 2011), whereas the other two studies (originating from the same group) described an intrinsic expression of TRPV1 in the guinea pig ENS (Anavi-Goffer et al 2002;Anavi-Goffer and Coutts 2003). These different findings might be related to species differences.…”

Section: Discussionmentioning

confidence: 98%

“…Based on previous studies in the mouse by our group and others and the limited IR in perikarya, these fibers are presumed to originate extrinsically in the spinal and vagal ganglia (De Jonge et al 2003;Ward et al 2003;Matsumoto et al 2011). The intracellular staining by Gp-anti-TRPV1 hints at a splice variant because this splicing affects translocation to the membrane (Hellwig et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, the effects of TRPV1 on GI functioning have mostly been linked to its expression in extrinsic nerve fibers. Regardless of GI region or species, TRPV1 is widely known to be expressed in afferent nerve fibers (Guo et al 1999;Yiangou et al 2001;Poonyachoti et al 2002;Anavi-Goffer and Coutts 2003;Chan et al 2003;De Jonge et al 2003;Ward et al 2003;Schicho et al 2004;Kadowaki et al 2004;Faussone-Pellegrini et al 2005;Matsumoto et al 2011). Some of these studies also reported an intrinsic expression of TRPV1 in the GI tract of several mammalian species (Akiba et al 2001;Kulkarni-Narla and Brown 2001;AnaviGoffer et al 2002;Poonyachoti et al 2002;Anavi-Goffer and Coutts 2003;Chan et al 2003;Faussone-Pellegrini et al 2005), as further corroborated by a physiological study in the mouse by Penuelas et al (2007).…”

mentioning

confidence: 84%

See 3 more Smart Citations

Transient Receptor Potential Vanilloid Type 1 Channel (TRPV1) Immunolocalization in the Murine Enteric Nervous System Is Affected by the Targeted C-terminal Epitope of the Applied Antibody

Buckinx

Nassauw

Avula

et al. 2013

J Histochem Cytochem.

View full text Add to dashboard Cite

The expression of transient receptor potential vanilloid type 1 channel (TRPV1) in the enteric nervous system is still the subject of debate. Although a number of studies have reported that TRPV1 is limited to extrinsic afferent fibers, other studies argue for an intrinsic expression of TRPV1. In the present study, reverse transcriptase PCR was employed to establish the expression of TRPV1 mRNA throughout the gastrointestinal tract. Using two antibodies directed against different epitopes of TRPV1, we were able to show at the protein level that the observed distribution pattern of TRPV1 is dependent on the antibody used in the immunohistochemical staining. A first antibody indeed mainly stained neuronal fibers, whereas a second antibody exclusively stained perikarya of enteric neurons throughout the mouse gastrointestinal tract. We argue that these different distribution patterns are due to the antibodies discriminating between different modulated forms of TRPV1 that influence the recognition of the targeted immunogen and as such distinguish intracellular from plasmalemmal forms of TRPV1. Our study is the first to directly compare these two antibodies within the same species and in identical conditions. Our observations underline that detailed knowledge of the epitope that is recognized by the antibodies employed in immunohistochemical procedures is a prerequisite for correctly interpreting experimental results.

show abstract

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 84%

See 2 more Smart Citations

Transient Receptor Potential Vanilloid Type 1 Channel (TRPV1) Immunolocalization in the Murine Enteric Nervous System Is Affected by the Targeted C-terminal Epitope of the Applied Antibody

Buckinx

Nassauw

Avula

et al. 2013

J Histochem Cytochem.

View full text Add to dashboard Cite

show abstract

“…Our findings show that TRPV-1 contributes to protection of tumor development in the lower gastrointestinal tract of mice subjected to chronic inflammation and in mice genetically predisposed to develop colon adenomas, which implies that TRPV-1 may be also relevant to modulate the homeostasis of the colonic immune system. It has been recently shown that TRPV-1-immunoreactive nerve fibers present in the rectum colocalize with CGRP, confirming the neuropeptidergic nature of TRPV-1 expressing sensory fibers (38). However, it has been reported that colon neurons expressing markers for A-and C-fibers express VIP but not TRPV-1, suggesting the existence of separate populations of neurons projecting from colon to CNS (39).…”

Section: Discussionmentioning

confidence: 85%

Vanilloid Receptor-1 Regulates Neurogenic Inflammation in Colon and Protects Mice from Colon Cancer

et al. 2012

View full text Add to dashboard Cite

Neuroinflammation driven by the vanilloid-type ion channel receptor transient receptor potential vanilloid type 1 (TRPV-1) is suspected to play a role in the pathophysiology of inflammatory bowel disease. Because inflammatory bowel disease is known to elevate the risk of colon cancer, we examined postulated roles for TRPV-1-driven neuroinflammation in promoting colitis-associated and spontaneous colon cancer development. Using a well-established model of colitis-associated cancer (CAC), we found that mice genetically deficient in TRPV-1 showed a higher incidence and number of tumors in the distal colon. In like manner, genetic deficiency of TRPV-1 in the APC Min/þ model of spontaneous colon cancer accentuated the number of colonic adenomas formed.Mechanistic analyses in the CAC model revealed an increased infiltration of inflammatory cells into the tumors along with elevated expression of interleukin (IL)-6 and IL-11 and activation of the STAT3 and NF-kB signaling pathways. Notably, TPRV-1-deficient mice exhibited a defect in expression of the anti-inflammatory neuropeptides, vasoactive intestinal peptide (VIP), and pituitary adenylate cyclase-activating peptide (PACAP) which contributed to the generation of a local proinflammatory environment. Together, our findings argue that by limiting neuroinflammatory processes, TRPV-1 exerts a protective role that restricts the initiation and progression of colon cancer. Cancer Res; 72(7);

show abstract

TRPV1 in Cell Signaling: Molecular Mechanisms of Function and Modulation

Rosenbaum

Jara-Oseguera

2012

Mechanically Gated Channels and Their Regulation

View full text Add to dashboard Cite

Distribution of transient receptor potential vanilloid 1 channel-expressing nerve fibers in mouse rectal and colonic enteric nervous system: relationship to peptidergic and nitrergic neurons

Cited by 58 publications

References 50 publications

Transient Receptor Potential Vanilloid Type 1 Channel (TRPV1) Immunolocalization in the Murine Enteric Nervous System Is Affected by the Targeted C-terminal Epitope of the Applied Antibody

Transient Receptor Potential Vanilloid Type 1 Channel (TRPV1) Immunolocalization in the Murine Enteric Nervous System Is Affected by the Targeted C-terminal Epitope of the Applied Antibody

Vanilloid Receptor-1 Regulates Neurogenic Inflammation in Colon and Protects Mice from Colon Cancer

TRPV1 in Cell Signaling: Molecular Mechanisms of Function and Modulation

Contact Info

Product

Resources

About