Distribution of the Phosphoenolpyruvate:Glucose Phosphotransferase System in Bacteria

Abstract

A survey of the occurrence of the phosphoenolpyruvate-dependent glucose phosphotransferase system was carried out in a number of bacteria, representing both gram-positive and gram-negative facultative anaerobic and strictly aerobic types. The system was found to be present in representatives of genera that are characteristically facultative anaerobes, but the system was absent in members of those genera that are strictly aerobic. Thus, although the phosphoenolpyruvate phosphotransferase system is an important system for the transport of sugars in bacteria carrying out anaerobic glycolysis, it plays no role in sugar transport by those organisms having a strictly oxidative physiology. A fundamentally different system, probably not involving phosphorylation during transport, is indicated in this latter group.

Insertion of anE. coli lacZgene inAcetobacter xylinusfor the production of cellulose in whey

“…The phosphoenolpyruvate (PEP)-dependent PTS was ¢rst reported to be absent in a strain of A. xylinus coherent with the ¢ndings by other workers that this system has no role in the transport of sugar in strictly aerobic organisms. Glucose and fructose utilisation for this particular A. xylinus strain involved the exclusive phosphorylation of glucose by a constitutive adenosine triphosphate (ATP)-dependent glucokinase, and of fructose by phosphorylation by an inducible ATP-dependent fructokinase, respectively [45,46]. More recently, PEP-dependent PTS Fruc has been described for A. xylinum BPR 2001 [47].…”

Bacterial phosphoenolpyruvate: sugar phosphotransferase systems: structural, functional, and evolutionary interrelationships.

“…DISTRIBUTION OF THE PTS IN THE PROCARYOTIC WORLD The presence of a PEP-dependent sugar PTS in eucaryotic organisms has not been convincingly demonstrated. However, the system has been found to be widely distributed in the procaryotic world (24). Table 2 summarizes the information presently available.…”

Phosphoenolpyruvate‐dependent glucose phosphotransferase activity in Streptococcus mitis ATCC 903

“…The phosphoryiating activity detected with ATP' as, the energy source contrasts with previous studies using Strep, mutans (21,22) and Strep, salivarius (9) where no such phosphorylation was observed. This activity may represent phosphorylation of 2DG by a hexokinase, as suggested for the low acdvity found for Pseudomonas aeruginosa (16). The hexokinase would only be directly involved in sugar catabolism if the free sugar is transported.…”

“…Glucose PTS activity was determined essentially by the method of ROMANO et al (16). Decryptified cells were prepared according to HAMILTON &.…”