Distribution of the cysteinyl leukotriene system components in the human, rat and mouse eye

Brunner, Susanne M.; Schrödl, Falk; Preishuber-Pflügl, Julia; Runge, Christian; Koller, Andreas; Lenzhofer, Markus; Reitsamer, Herbert A.; Trost, Andrea

doi:10.1016/j.exer.2023.109517

Cited by 4 publications

(2 citation statements)

References 70 publications

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“…A homozygous region of length 21,063,873-22,280,900, including the genes associated with muscle, ligament, and tendon development (TRIM13, SPRYD7, and SETDB2), was found at ECA17 in horses of the Trakenen breed [66], which influences the persistence of inflammatory joint disease along an ontology structure similar to humans (EBPL) [67], the presence of cognitive abilities (KPNA3 and RCBTB1) [68,69] and immune response (PHF11 and CYSLTR2) [70,71].…”

Section: Discussionmentioning

confidence: 99%

The Genetic Diversity of Stallions of Different Breeds in Russia

Дементьева

Nikitkina

Shcherbakov

et al. 2023

Genes

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The specifics of breeding and selection significantly affect genetic diversity and variability within a breed. We present the data obtained from the genetic analysis of 21 thoroughbred and warmblood horse breeds. The most detailed information is described from the following breeds: Arabian, Trakehner, French Trotter, Standardbred, and Soviet Heavy Horse. The analysis of 509,617 SNP variants in 87 stallions from 21 populations made it possible to estimate the genetic diversity at the genome-wide level and distinguish the studied horse breeds from each other. In this study, we searched for heterozygous and homozygous ROH regions, evaluated inbreeding using FROH analysis, and generated a population structure using Admixture 1.3 software. Our findings indicate that the Arabian breed is an ancestor of many horse breeds. The study of the full-genome architectonics of breeds is of great practical importance for preserving the genetic characteristics of breeds and managing breeding. Studies were carried out to determine homozygous regions in individual breeds and search for candidate genes in these regions. Fifty-six candidate genes for the influence of selection pressure were identified. Our research reveals genetic diversity consistent with breeding directions and the breeds’ history of origin.

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Section: Discussionmentioning

confidence: 99%

The Genetic Diversity of Stallions of Different Breeds in Russia

Дементьева

Nikitkina

Shcherbakov

et al. 2023

Genes

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“…Therefore, the main goal of this current work was to determine the impact of autophagy induction through CysLTR1 inhibition on aggregated protein degradation and ROS formation in polarized ARPE-19 cells. Furthermore, we examined the potential of targeting autophagic flux via CysLTR1 antagonization to improve resilience to stressors in ocular aging and disease models, especially as CysLTR1 is highly expressed in the retina 34 .…”

Section: Introductionmentioning

confidence: 99%

Inhibition of CysLTR1 reduces the levels of aggregated proteins in retinal pigment epithelial cells

Koller,

Brunner,

Preishuber-Pflügl

et al. 2023

Sci Rep

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The endosomal-lysosomal system (ELS), which carries out cellular processes such as cellular waste degradation via autophagy, is essential for cell homeostasis. ELS inefficiency leads to augmented levels of damaged organelles and intracellular deposits. Consequently, the modulation of autophagic flux has been recognized as target to remove damaging cell waste. Recently, we showed that cysteinyl leukotriene receptor 1 (CysLTR1) antagonist application increases the autophagic flux in the retinal pigment epithelial cell line ARPE-19. Consequently, we investigated the effect of CysLTR1 inhibition–driven autophagy induction on aggregated proteins in ARPE-19 cells using flow cytometry analysis. A subset of ARPE-19 cells expressed CysLTR1 on the surface (SE+); these cells showed increased levels of autophagosomes, late endosomes/lysosomes, aggregated proteins, and autophagy as well as decreased reactive oxygen species (ROS) formation. Furthermore, CysLTR1 inhibition for 24 h using the antagonist zafirlukast decreased the quantities of autophagosomes, late endosomes/lysosomes, aggregated proteins and ROS in CysLTR1 SE- and SE+ cells. We concluded that high levels of plasma membrane–localized CysLTR1 indicate an increased amount of aggregated protein, which raises the rate of autophagic flux. Furthermore, CysLTR1 antagonist application potentially mimics the physiological conditions observed in CysLTR1 SE+ cells and can be considered as strategy to dampen cellular aging.

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