Distribution of the calcium-binding proteins parvalbumin and calbindin-D28k in the sensorimotor cortex of the rat

Brederode, J. F. M. Van; helliesen, M.K.; Hendrickson, Anita E.

doi:10.1016/0306-4522(91)90258-p

Cited by 172 publications

(107 citation statements)

References 40 publications

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“…This result is consistent with the report that 14% of all neurons were parvalbumin-immunoreactive in the rat somatosensory cerebral cortex (Ren et al, 1992). Because parvalbumin is found virtually only in GABA neurons in the cerebral cortex (Celio, 1986(Celio, , 1990van Brederode et al, 1991;Ren et al, 1992), type 2A neurons must be GABAergic. This is consistent with the finding via the single-cell PCR technique that the type II neurons corresponding to type 2A neurons in cultured hippocampal neurons contained glutamic acid decarboxylase (GAD) mRNA (Bochet et al, 1994).…”

Section: The Relation Between the Four Types Of Cortical Neurons And supporting

confidence: 92%

Combinations of AMPA Receptor Subunit Expression in Individual Cortical Neurons Correlate with Expression of Specific Calcium-Binding Proteins

1997

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The functional properties of AMPA-type glutamate receptors are determined by their subunit composition. We detected the expression of the AMPA receptor subunits (GluR1-GluR4) in neurons in the somatosensory cortex of adult rats by combining nonradioactive in situ hybridization using digoxigenin-labeled RNA probes of GluR1 and GluR2 with immunocytochemistry using specific antibodies against GluR1, GluR2/3, and GluR4. On the basis of differential expression of the GluR1 and GluR2 subunits, we classified the cortical neurons into four categories. To correlate the differential expression of AMPA receptor subunits in each neuron with that of two calcium-binding proteins, parvalbumin and calbindin-D28k, we used a triple-labeling method. The majority of cortical neurons (ϳ2/3) showed expression of GluR2 and undetectable expression of GluR1. GluR1-/GluR2-expressing neurons and GluR1-expressing/GluR2-undetectable neurons comprised ϳ1/10 each. Regarding the morphology, most GluR1-undetectable/GluR2-expressing neurons were pyramidal cells in layers II/III, V, and VI, whereas most GluR1-expressing/GluR2-undetectable neurons were nonpyramidal cells in layers II-VI. The GluR1-/GluR2-expressing neurons were either pyramidal or nonpyramidal. The majority of GluR1-/GluR2-expressing nonpyramidal cells was intensely stained with monoclonal antibody against calbindin-D28k, and one-half of the GluR1-undetectable/GluR2-expressing pyramidal neurons in layer II/III were lightly stained with this antibody. Most of GluR1-expressing/GluR2-undetectable neurons possessed parvalbumin immunoreactivity. These results indicate that neurons in the rat somatosensory cortex express differential combinations of GluR subunits, which correlate with the specific expression of the calcium-binding proteins.

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Section: The Relation Between the Four Types Of Cortical Neurons And supporting

confidence: 92%

Combinations of AMPA Receptor Subunit Expression in Individual Cortical Neurons Correlate with Expression of Specific Calcium-Binding Proteins

1997

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“…However, a wealth of data indicate that pyramidal and nonpyramidal neurons are distinct populations of postmitotic cells since their generation and, in accordance with a number of lineage studies (Parnavelas et al, 199 1;Luskin et al, 1993) even before. The development of the Glu-and GABAimmunoreactive neurons in the embryonic cortex is consistent with the notion of two classes of pyramidal and nonpyramidal neurons of the adult cortex (Lauder et al, 1986;Erdii and Wolff, 1989;Van Eden et al, 1989;Blanton and Kriegstein, 1991;Cobas et al, 1991). It has been suggested that the two neurotransmitters play critical roles in development by regulating differentiation and survival of other cells (Mattson, 1988;Mattson and Kater, 1989;for review, see Redburn and Schousboe, 1987;McDonald and Johnston, 1990).…”

Section: Assignment Of Clonal Boundariessupporting

confidence: 82%

Lineage analysis reveals neurotransmitter (GABA or glutamate) but not calcium-binding protein homogeneity in clonally related cortical neurons

et al. 1994

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Studies of cell lineage in the rat cerebral cortex have provided new insights into the mechanisms of neuronal and glial determination. They have shown that clonally related cells, marked with retrovirus injection at embryonic day 16 (E16), express the same glial or neuronal phenotype, suggesting that separate progenitors for each of these cell phenotypes exist in the ventricular zone at that stage of corticogenesis. However, it is not known if such committed progenitors are present in the ventricular zone before E16. Another important question concerns which neurochemical features are shared by clonally related cells of the adult cerebral cortex. In this study we have addressed the first question by injecting a retroviral vector expressing beta-galactosidase into the telencephalic ventricles of rat embryos at different stages (E14-E19). In order to classify clonally related neurons in the cerebral cortex of these rats, we have used postembedding immunohistochemistry for the amino acid neurotransmitters glutamate, aspartate, and GABA. Glutamate and GABA immunoreactivity marked nonoverlapping populations of cells that corresponded to the pyramidal and nonpyramidal neuron types of the rat cerebral cortex. Clonally related neurons, marked by retrovirus injection at any day between E14 and E19, homogeneously expressed one or other phenotype and accordingly displayed glutamate or GABA immunoreactivity. This finding indicates that committed progenitor cells for pyramidal and nonpyramidal neurons are present in the ventricular zone before E16. To investigate whether lineage dictates other features in clonally related neurons, we performed an immunohistochemical analysis for the calcium- binding proteins calbindin, parvalbumin, and calretinin in clusters of clonally related nonpyramidal neurons. The same calcium-binding protein was rarely found in members of the same cluster, suggesting that lineage does not control the expression of calcium-binding proteins in cortical nonpyramidal neurons. As a result of examining a large number of clonally related neurons from brains injected at different ages, we observed remarkable differences in number and laminar distribution of pyramidal and nonpyramidal neurons marked with retrovirus. Clusters of nonpyramidal neurons were usually composed of two or three cells, and resided in the cortical layers that were just being generated at the time of injection. Clusters of pyramidal neurons were larger and dispersed in several layers in the earlier injections; their size and laminar distribution were progressively reduced for later injections. These observations suggest the existence of different mechanisms that generate the pyramidal and nonpyramidal neurons of the cerebral cortex.

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“…This suggests a similar proportion of FSUs and RSUs in AI of cat and rabbit. Lower proportions of FSUs in layers 2/3 and upper layer 5 also echo distributions obtained in somatosensory and visual cortex (Demeulemeester et al, 1989;van Brederode et al, 1991).…”

Section: Classification Of Fsus and Rsussupporting

confidence: 69%

Spectrotemporal Processing Differences between Auditory Cortical Fast-Spiking and Regular-Spiking Neurons

Atencio¹,

Schreiner²

2008

J. Neurosci.

109

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Excitatory pyramidal neurons and inhibitory interneurons constitute the main elements of cortical circuitry and have distinctive morphologic and electrophysiological properties. Here, we differentiate them by analyzing the time course of their action potentials (APs) and characterizing their receptive field properties in auditory cortex. Pyramidal neurons have longer APs and discharge as regular-spiking units (RSUs), whereas basket and chandelier cells, which are inhibitory interneurons, have shorter APs and are fast-spiking units (FSUs). To compare these neuronal classes, we stimulated cat primary auditory cortex neurons with a dynamic moving ripple stimulus and constructed single-unit spectrotemporal receptive fields (STRFs) and their associated nonlinearities. FSUs had shorter latencies, broader spectral tuning, greater stimulus specificity, and higher temporal precision than RSUs. The STRF structure of FSUs was more separable, suggesting more independence between spectral and temporal processing regimens. The nonlinearities associated with the two cell classes were indicative of higher feature selectivity for FSUs. These global functional differences between RSUs and FSUs suggest fundamental distinctions between putative excitatory and inhibitory interneurons that shape auditory cortical processing.

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Distribution of the calcium-binding proteins parvalbumin and calbindin-D28k in the sensorimotor cortex of the rat

Cited by 172 publications

References 40 publications

Combinations of AMPA Receptor Subunit Expression in Individual Cortical Neurons Correlate with Expression of Specific Calcium-Binding Proteins

Combinations of AMPA Receptor Subunit Expression in Individual Cortical Neurons Correlate with Expression of Specific Calcium-Binding Proteins

Lineage analysis reveals neurotransmitter (GABA or glutamate) but not calcium-binding protein homogeneity in clonally related cortical neurons

Spectrotemporal Processing Differences between Auditory Cortical Fast-Spiking and Regular-Spiking Neurons

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