Distribution of Tetracycline Resistance Genes and Transposons among Phylloplane Bacteria in Michigan Apple Orchards

Schnabel, Elise; Jones, Alan L.

doi:10.1128/aem.65.11.4898-4907.1999

Cited by 133 publications

(72 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lack of transfer of other tet genes in the present study may be because of the fact that only two different recipients were tested. Moreover, we also recognize that in future studies the association between detected genes and transposons should be investigated in order to better characterize their potential dissemination (Schnabel and Jones 1999).…”

Section: Discussionmentioning

confidence: 99%

Tetracycline-resistance genes in Gram-negative isolates from estuarine waters

Henriques

Fonseca

Alves

et al. 2008

Letters in Applied Microbiology

View full text Add to dashboard Cite

Aims: To investigate the diversity and dissemination of tetracycline resistance genes in isolates from estuarine waters. Methods and Results: Forty‐two out of 164 multi‐resistant isolates previously obtained were resistant or less‐susceptible to tetracycline, as evaluated by the disc diffusion method. Minimal inhibitory concentration for resistant bacteria ranged from 16 to 256 mg l−1. Screening of tet genes by polymerase chain reaction showed that 88% of the isolates carried at least one of the genes tested, namely tet(A) (present in 13 isolates), tet(B) (present in 13 isolates), tet(C) (present in 3 isolates), tet(D) (present in 1 isolate), tet(E) (present in 6 isolates) and tet(M) (present in 1 isolate). One isolate carried tet(A) and tet(M). To our knowledge, this study presents the first description of a tet(D) gene in Morganella morganii. Hybridization revealed that tet genes were plasmid‐located in 31% of the isolates. Those isolates were included as donors in conjugation experiments and 38% transferred tetracycline resistance. Conclusions: A considerable diversity of tet genes was detected in the estuary. Frequently, these genes were associated with plasmids and could be transferred to Escherichia coli. Significance and Impact of the Study: The results presented provide further evidence of the role played by estuarine reservoirs in antibiotic resistance maintenance and dissemination.

show abstract

Section: Discussionmentioning

confidence: 99%

Tetracycline-resistance genes in Gram-negative isolates from estuarine waters

Henriques

Fonseca

Alves

et al. 2008

Letters in Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…The fact that no e¡ect of selective pressure by Sm or copper treatment [38,41] was found can be explained by several hypotheses: (1) dilution or inactivation of the selective compounds, (2) a possible e¡ect was obscured by rapid decay of the Sm-resistant populations, or (3) the e¡ect was too small to be detected at all. Sm-resistant populations in natural habitats may £uctuate in time and space, occasionally dropping to below the limit of detection, and the populations targeted, which were pos-sibly favoured by selection, may have followed the same trends.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of streptomycin-resistance genes in bacterial populations in European habitats

VANOVERBEEK¹,

WELLINGTON²,

EGAN³

et al. 2002

FEMS Microbiology Ecology

View full text Add to dashboard Cite

The prevalence of selected streptomycin (Sm)-resistance genes, i.e. aph (3''), aph (6)-1d, aph (6)-1c, ant (3'') and ant (6), was assessed in a range of pristine as well as polluted European habitats. These habitats included bulk and rhizosphere soils, manure from farm animals, activated sludge from wastewater treatment plants and seawater. The methods employed included assessments of the prevalence of the genes in habitat-extracted DNA by PCR, followed by hybridisation with specific probes, Sm-resistant culturable bacteria and exogenous isolation of plasmids carrying Sm-resistance determinants. The direct DNA-based analysis showed that aph (6)-1d genes were most prevalent in the habitats examined. The presence of the other four Sm-modifying genes was demonstrated in 58% of the tested habitats. A small fraction of the bacterial isolates (8%) did not possess any of the selected Sm-modifying genes. These isolates were primarily obtained from activated sludge and manure. The presence of Sm-modifying genes in the isolates often coincided with the presence of IncP plasmids. Exogenous isolation demonstrated the presence of plasmids of 40-200 kb in size harbouring Sm-resistance genes from all the environments tested. Most plasmids were shown to carry the ant (3'') gene, often in combination with other Sm-resistance genes, such as aph (3'') and aph (6)-1d. The most commonly found Sm-modifying gene on mobile genetic elements was ant (3''). Multiple Sm-resistance genes on the same genetic elements appeared to be the rule rather than the exception. It is concluded that Sm-resistance genes are widespread in the environmental habitats studied and often occur on mobile genetic elements and ant (3'') was most often encountered.

show abstract

“…sobria and A. salmonicida . In these isolates, the presence of class A family Tet tetracycline resistance genes ( tet A, tet B, tet C, tetD, tet E and tet H) was assessed by PCR and HaeIII digestion of amplicons (Jacobs & Chenia ; Schnabel & Jones ). The tet E determinant was detected most frequently among the isolates (78%), while 22% of the isolates possessed tetD/H determinants.…”

Section: Effect Of Antibioticsmentioning

confidence: 99%

Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story?

et al. 2015

View full text Add to dashboard Cite

It is well known that healthy gut microbiota is essential to promote host health and well‐being. The intestinal microbiota of endothermic animals as well as fish are classified as autochthonous or indigenous, when they are able to colonize the host's epithelial surface or are associated with the microvilli, or as allochthonous or transient (associated with digesta or are present in the lumen). Furthermore, the gut microbiota of aquatic animals is more fluidic than that of terrestrial vertebrates and is highly sensitive to dietary changes. In fish, it is demonstrated that [a] dietary form (live feeds or pelleted diets), [b] dietary lipid (lipid levels, lipid sources and polyunsaturated fatty acids), [c] protein sources (soybean meal, krill meal and other meal products), [d] functional glycomic ingredients (chitin and cellulose), [e] nutraceuticals (probiotics, prebiotics, synbiotics and immunostimulants), [f] antibiotics, [g] dietary iron and [h] chromic oxide affect the gut microbiota. Furthermore, some information is available on bacterial colonization of the gut enterocyte surface as a result of dietary manipulation which indicates that changes in indigenous microbial populations may have repercussion on secondary host–microbe interactions. The effect of dietary components on the gut microbiota is important to investigate, as the gastrointestinal tract has been suggested as one of the major routes of infection in fish. Possible interactions between dietary components and the protective microbiota colonizing the digestive tract are discussed.

show abstract

Distribution of Tetracycline Resistance Genes and Transposons among Phylloplane Bacteria in Michigan Apple Orchards

Cited by 133 publications

References 56 publications

Tetracycline-resistance genes in Gram-negative isolates from estuarine waters

Tetracycline-resistance genes in Gram-negative isolates from estuarine waters

Prevalence of streptomycin-resistance genes in bacterial populations in European habitats

Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story?

Contact Info

Product

Resources

About