Distribution of T-lymphocyte subsets in Hodgkin's disease characterized by monoclonal antibodies

Abstract

Summary.-Mononuclear-cell suspensions of lymph nodes, spleen and blood from 24 patients with active Hodgkin's disease (HD) were studied for possible imbalance of T and B lymphocytes, and T-lymphocyte subsets, using monospecific anti-T antibodies and other reagents. A profile showing T-cell predominance was demonstrated ln lymph nodes and blood, with total T-cells ranging from 50-70% of the cell count. As defined by monoclonal antibodies, 70-85% of the latter comprised the "inducer" subclass, the remainder being "suppressor" cells. There were no essential differences between histologically involved and uninvolved lymph nodes from HD patients, though total T-cell proportions were lower in "normal lymph node" controls. The profiles of spleens electively removed, as part of pre-treatment staging procedures, showed reduced total T-cell numbers, whether these were involved with HD or not. These differences are accounted for principally by fewer T "inducer" cells (24%, in spleen, v. 54% in involved lymph nodes and 47% in "normal" control nodes).Possible explanations for these findings are discussed. Our results demonstrate similar profiles in histologically diseased and normal tissue, rather than any clear imbalance of T-cell proportions which might explain the profound disturbances of T-cell function frequently demonstrated in vivo and in vitro.

The Reed-Sternberg cell/lymphocyte rosette. I. Properties of rosettes formed between Hodgkin's cell lines and allogeneic lymphocytes

“…Morris & Stuart (1984) found that CD4+ T-cells attached more frequently to RS/HM cells than CD8 + T-cells in fresh cell suspensions prepared from Hodgkin's involved lymph nodes. Previous studies have shown that for the majority of HD cases of all subtypes studied, with the exception of the lymphocyte depleted subtype, the majority of the T-cell population in the involved node are of the CD4+ subset (Dorreen et al, 1982;Poppema et al, 1982;Abdulaziz et al, 1984) and thus, the apparently more frequent association of CD4 + T-cells with RS/HM cells may simply reflect the higher proportion of CD4+ cells available to compete for attachment.…”

The cellular content of non Hodgkin lymphomas: A comprehensive analysis using monoclonal antibodies and other surface marker techniques

“…Even T lymphoblastic lymphomas show some evidence of being composed of mixtures of phenotypically distinct subsets of cells, which may mimic the normal patterns of phenotypic variation occurring during intrathymic differentiation. The T cell component in Hodgkin's Disease (Dorreen et al, 1982) or in follicular lymphoma is often not demonstrably abnormal in respect of the relative proportions of T "helper" or "suppressor" cells, or in their distribution as assessed by frozen section. Nonetheless, mixtures of such cells do, in individual patients, show the capacity for progressive growth, ultimately with the emergence of a dominant B cell population showing light chain class restriction, and probably derived from a single B cell clone.…”

Generation of human monoclonal antibodies recognising membranous antigens of the lung adenocarcinoma cell line A549 using an AMeX immunohistostaining method

“…For 28K29 and 28B49 (a negative control) cells, HT was further added to the serumfree medium. (Dorreen et al, 1982). Then, the wells were washed three times with PBS containing 0.05% Tween 20 (Tween 20/PBS) and blocked with PBS containing 3% BSA.…”