Distribution of T-lymphocyte Subpopulation in Blood and Spleen of Normal Cattle and Cattle with Enzootic Bovine Leukosis

Wu, D.; Takahashi, K.; Liu, N.; Koguchi, A.; Makara, Manami; Sasaki, Jun; Goryo, Masanobu; Okada, Kōsuke

doi:10.1053/jcpa.1998.0259

Cited by 17 publications

(16 citation statements)

References 24 publications

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“…The distribution and population of immunocompetent cells in these lymphoid organs has been well established [7,14,20,29,37,38]. However, the distribution and population of immunocompetent cells in HNs have not been sufficiently investigated to understand the exact role of HN in the immune system.…”

Section: Discussionmentioning

confidence: 99%

Comparative Studies on the Distribution and Population of Immunocompetent Cells in Bovine Hemal Node, Lymph Node and Spleen

Zhang

Nasu

Hosaka

et al. 2012

The Journal of Veterinary Medical Science

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abstract. The distribution and population of immunocompetent cells in bovine hemal node, mesenteric lymph node and spleen were analyzed comparatively by immunohistochemistry and flow cytometry. Many CD8 + cells, CD172a + cells and γδ T cells were found in the lymphatic cord along the sinus of the hemal node and the splenic red pulp. A few CD8 + cells and γδ T cells were distributed diffusely in the paracortex and medullary cord of the mesenteric lymph node. Many germinal centers were recognized in the lymphatic regions such as the cortex and white pulp of these lymphoid organs. The populations of CD8 + cells and γδ T cells in the hemal node and the spleen were higher than those of the mesenteric lymph node. In addition, the populations of CD21 + cells and MHC class II + cells in the hemal node and the mesenteric lymph node were higher than those of the spleen. The results suggest that the hemal node has an important role in both cellular and humoral immunity as well as the lymph node and the spleen in cattle.

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Section: Discussionmentioning

confidence: 99%

Comparative Studies on the Distribution and Population of Immunocompetent Cells in Bovine Hemal Node, Lymph Node and Spleen

Zhang

Nasu

Hosaka

et al. 2012

The Journal of Veterinary Medical Science

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“…4 A proportion of cattle infected with bovine leukemia virus develop persistent lymphocytosis, which is composed of CD5 þ and surface immunoglobulin M þ polyclonal lymphocytes, whereas solid tumors express CD5, surface immunoglobulin M, MHC class II, and only a single light-chain type. 89 …”

Section: Lymphoproliferative Diseasementioning

confidence: 99%

Flow Cytometry in Veterinary Oncology

Reggeti

Bienzle

2010

Vet Pathol

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Flow cytometry is a highly sensitive and specific method for simultaneous analysis of multiple parameters of individual cells in a suspension. It has a range of applications in veterinary medicine, and it is increasingly used in veterinary oncology as more species-specific antibodies are generated and cross-reactivity of antibodies is characterized. Two major applications in veterinary oncology are (1) immunophenotyping with a panel of fluorescently labeled antibodies to assess expression of cell markers and (2) determination of the DNA content of cells with fluorescent dyes that bind nucleic acids. The diagnostic and prognostic value of classifying round cell tumors of animals-especially, lymphocyte proliferations-remains to be fully determined, but studies to date have indicated benefit to patient management. Similarly, determining the proliferating fraction of tumors through DNA analysis remains to be standardized and validated in veterinary oncology but shows promise as an adjunct to morphologic tumor classification. This article reviews technical aspects of flow cytometry, availability of antibodies suitable for studies in domestic animals, and applications in veterinary oncology with emphasis on characterization of round cell tumors.

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“…However, these neoplastic B cells are classified into different phenotypes by immunohistochemical analysis using a monoclonal antibody to leukocyte differentiation antigen [6,7,13,42,43,46]. Furthermore, tumor cells in EBL were classified into three or more types on the basis of histological findings [17,39].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Analysis of Expression Patterns of Tumor Necrosis Factor Receptors on Lymphoma Cells in Enzootic Bovine Leukosis

Ikeda

Konnai

Onuma

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Tumor necrosis factor-alpha (TNF-α) has been reported to be associated with the progression of lymphoproliferative neoplastic diseases and retroviral infections. Hence we examined immunohistochemically the expression patterns of TNF-receptors (TNF-RI and RII) on lymphoma cells derived from the 29 cases of enzootic bovine leukosis (EBL). Lymphomas obtained in 29 animals with EBL were histopathologically classified into three types: diffuse mixed type (10 cases), diffuse large type (9 cases), and diffuse large cleaved type (10 cases). Immunohistochemically using a monoclonal antibody to a bovine lymphocyte surface antigen, the lymphomas were classified into three phenotypes: B-1a (CD5 + /CD11b + ), B-1b (CD5 -/CD11b + ) and B-2 (conventional B) (CD5 -/CD11b -). Interestingly, the lymphoma cells in all animals expressed TNF-RII, but not TNF-RI. Although, in EBL, lymphoma cells of which the histopathological and immunological property differs has been formed, the expression patterns of TNF-Rs had the universality in all lymphoma cells. TNF-RII, which induces cell proliferation, was expressed but TNF-RI, which induces cell apoptosis was not expressed on all lymphoma cells, suggesting that TNF-Rs play an important role in the malignant proliferation of B cells and formation of lymphomas in EBL. KEY WORDS: enzootic bovine leukosis, lymphoma, phenotype, tumor necrosis factor-α, tumor necrosis factor receptor.

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Distribution of T-lymphocyte Subpopulation in Blood and Spleen of Normal Cattle and Cattle with Enzootic Bovine Leukosis

Cited by 17 publications

References 24 publications

Comparative Studies on the Distribution and Population of Immunocompetent Cells in Bovine Hemal Node, Lymph Node and Spleen

Comparative Studies on the Distribution and Population of Immunocompetent Cells in Bovine Hemal Node, Lymph Node and Spleen

Flow Cytometry in Veterinary Oncology

Immunohistochemical Analysis of Expression Patterns of Tumor Necrosis Factor Receptors on Lymphoma Cells in Enzootic Bovine Leukosis

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