Distribution of some adsorbed and intrinsic enzymes between the mucosal cells of the rat small intestine and the apical glycocalyx separated from them

Ugolev, A. M.; Smirnova, L F; Iezuitova, N N; Тимофеева, Н. А.; Mityushova, N.M.; Egorova, V. V.; Паршков, Е. М.

doi:10.1016/0014-5793(79)81080-7

Cited by 32 publications

(11 citation statements)

References 5 publications

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“…This fully agrees with results obtained with rats using the same method [ 10]. Similarly to alkaline phosphatase, 16.1% of the CaBP is removed from the intestinal mucosa by the replica.…”

Section: Discussionsupporting

confidence: 90%

“…Electron microscopic investigations in [ 10] showed that forming an agar replica in rat intestinal segments left intact the ultrastructure of the epithelium cells and the brush border. Similar electron micrographs have been obtained for chick intestine in our laboratory [ 16].…”

Section: Discussionmentioning

confidence: 99%

“…Agar replicas were obtained according to [10]. After decapitation, the abdomen of the chick was opened, the duodenum removed and rinsed with an ice-cold solution of NaC1 (0.75%).…”

Section: Methodsmentioning

confidence: 99%

“…Here, we report the application to the problem of CaBP localization of a new and direct method, the agar replica method [ 10] to study the distribution of some enzymes in the intestinal mucosa.…”

Section: Introductionmentioning

confidence: 99%

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Detection of vitamin D‐dependent calcium‐binding protein in the region of apical glycocalyx of chick enterocytes

Galvanovsky¹,

Valinietse²,

Bauman³

1981

FEBS Letters

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“…This fully agrees with results obtained with rats using the same method [ 10]. Similarly to alkaline phosphatase, 16.1% of the CaBP is removed from the intestinal mucosa by the replica.…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

“…Agar replicas were obtained according to [10]. After decapitation, the abdomen of the chick was opened, the duodenum removed and rinsed with an ice-cold solution of NaC1 (0.75%).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of vitamin D‐dependent calcium‐binding protein in the region of apical glycocalyx of chick enterocytes

Galvanovsky¹,

Valinietse²,

Bauman³

1981

FEBS Letters

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“…It has been reported that the pancreatic enzymes capable of degrading proteins resided in the mucous layer. 21,22) Hence, the effect of PLA may also be influenced by the enzymes in the mucous layer. 20) The highest F 0-4 h was obtained in the jejunum when aprotinin was co-administered with PLA.…”

Section: Methodsmentioning

confidence: 99%

Effect of Poly-<small>L</small>-arginine on Intestinal Absorption of Hydrophilic Macromolecules in Rats

Yamaki

Uchida

Kuwahara

et al. 2013

Biological & Pharmaceutical Bulletin

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We have already reported that poly-l-arginine (PLA) remarkably enhanced the in vivo nasal absorption of hydrophilic macromolecules without producing any significant epithelial damage in rats. In the present study, we examined whether PLA could enhance the absorption of a model hydrophilic macromolecule, fluorescein isothiocyanate-dextran (FD-4), across the intestinal mucosa, as well as the nasal mucosa, by an in situ closed-loop method using the rat intestine. PLA was found to enhance the intestinal absorption of FD-4 in a concentration-dependent manner within the concentrations investigated in this study, but segment-specific differences were found to be associated with this effect (ileum>jejunum>duodenum≧colon). The factors responsible for the segment-specific differences were also investigated by intestinal absorption studies using aprotinin, a trypsin inhibitor, and an analysis of the expression of occludin, a tight junction protein. In the small intestine, the differences in the effect of PLA on the absorption of FD-4 may be related to the enzymatic degradation of PLA. In the colon, the reduced effect of PLA on the absorption of FD-4 may be related to the smaller surface area for absorption and the higher expression of occludin compared with other segments.Key words poly-l-arginine; intestinal absorption; enhancer; fluorescein isothiocyanate-dextran (FD-4); occludin; aprotininWe have already reported that poly-l-arginine (PLA), a polycation, remarkably enhanced the in vivo nasal absorption of hydrophilic macromolecules in rats and increased their in vitro permeation across the rabbit nasal epithelium without producing any significant epithelial damage. [1][2][3][4][5] This effect is caused by the opening of tight junctions (TJ) in the epithelial cells. PLA plays an important role in opening the TJ in these cells, and the reaction induced by PLA appears to be reversible.4,6) Therefore, PLA is considered to be promising as an absorption enhancer for transnasal drug delivery. Although some studies have examined whether PLA can also enhance the absorption of hydrophilic macromolecules across the nasal epithelium, conjunctiva, alveolar epithelium, and Caco-2 cell sheets, [7][8][9][10] there have been no studies looking at PLA᾿s absorption enhancing effect across the intestinal mucosa. The intestinal mucosa is an important tissue particularly for the absorption of orally administered drugs.The intestinal absorption of hydrophilic macromolecules is well known to be typically very poor.11) The small intestine has an extensive surface area for absorption, but various digestive enzymes exist in the gastrointestinal tract. The property of intestinal mucosa differs from that of the nasal mucosa in some respects. Therefore, in order to improve the absorption of hydrophilic macromolecules, various strategies such as the use of absorption enhancers, protease inhibitors and chemical modification have been examined in the intestinal mucosa as well as the nasal mucosa. [12][13][14][15] It was recently reported that the TJ ba...

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Aminopeptidases and Proteolipids of Intestinal Brush Border

Maroux

Feracci

Gorvel

et al. 1983

Ciba Foundation Symposium 95 ‐ Brush Border Membranes

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Distribution of some adsorbed and intrinsic enzymes between the mucosal cells of the rat small intestine and the apical glycocalyx separated from them

Cited by 32 publications

References 5 publications

Detection of vitamin D‐dependent calcium‐binding protein in the region of apical glycocalyx of chick enterocytes

Detection of vitamin D‐dependent calcium‐binding protein in the region of apical glycocalyx of chick enterocytes

Effect of Poly-<small>L</small>-arginine on Intestinal Absorption of Hydrophilic Macromolecules in Rats

Aminopeptidases and Proteolipids of Intestinal Brush Border

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