Distribution of serotonin and dopamine in the central nervous system of the female mud crab, Scylla olivacea (Herbst)

Khornchatri, Kanjana; Kornthong, Napamanee; Saetan, Jirawat; Tinikul, Yotsawan; Chotwiwatthanakun, Charoonroj; Cummins, Scott F.; Hanna, Peter; Sobhon, Prasert

doi:10.1016/j.acthis.2014.12.006

Cited by 20 publications

(19 citation statements)

References 42 publications

(48 reference statements)

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“…Previously researchers have localized both serotonin and dopamine immunoreactivity in female mud crab brain. 36 To the best of our knowledge, our study represents the first study on crustacean brain that allows simultaneous visualization of multiple primary amine neurotransmitters and metabolites. The crab brain anatomy (Supporting Information Figure S1) facilitates better understanding of tissue-specific in situ distribution.…”

Section: Resultsmentioning

confidence: 97%

Visualization and Identification of Neurotransmitters in Crustacean Brain via Multifaceted Mass Spectrometric Approaches

Cao

Wang

Chen

et al. 2019

ACS Chem. Neurosci.

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Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has emerged as a label-free analytical tool for fast biomolecule profiling on tissue sections. Among various functional molecules, mapping neurotransmitters and related metabolites is of tremendous significance, as these compounds are critical to signaling in the central nervous system. Here, we demonstrated the use of both derivatization and reaction-free approaches that greatly reduced signal complexity and thus enabled complementary signaling molecule visualization on crab brain sections via MALDI-LTQ-Orbitrap XL platform. Pyrylium salt served as a primary amine derivatization reagent and produced prominent signal enhancement of multiple neurotransmitters, including dopamine, serotonin, γ-aminobutyric acid, and histamine that were not detected in underivatized tissues. Molecules with other functional groups, such as acetylcholine and phosphocholine, were directly imaged after matrix application. The identities of discovered neurotransmitters were verified by standards using LC-MS/MS. This study broadens our understanding of metabolic signaling in the crustacean nervous system and highlights potential of multifaceted MS techniques for unambiguous neurotransmitter characterization.

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Section: Resultsmentioning

confidence: 97%

Visualization and Identification of Neurotransmitters in Crustacean Brain via Multifaceted Mass Spectrometric Approaches

Cao

Wang

Chen

et al. 2019

ACS Chem. Neurosci.

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“…Serotonin (5-HT) and dopamine (DA) are the neurotransmitter or neurohormones that synthesized from the eyestalks (Kornthong et al, 2019). The 5-HT and DA activity has been recognized in central nervous system (CNS), eyestalk, brain and thoracic ganglia (Khornchatri et al, 2015). Numerous physiological functions, including reproduction, are modulated by these neurotransmitters (Khornchatri et al, 2015).…”

Section: Neurotransmittersmentioning

confidence: 99%

“…The 5-HT and DA activity has been recognized in central nervous system (CNS), eyestalk, brain and thoracic ganglia (Khornchatri et al, 2015). Numerous physiological functions, including reproduction, are modulated by these neurotransmitters (Khornchatri et al, 2015).…”

Section: Neurotransmittersmentioning

confidence: 99%

“…5-HT has been studied extensively in the crustacean with various functions such as growth, aggression, behaviours, neuronal activity, neurogenesis, colour changes, osmotic adjustments and ovarian maturation (Nakeima et al, 2020). 5-HT acted as a reproductive inducer in both females and males, stimulating ovarian maturation and increased testicular growth (Khornchatri et al, 2015). 5-HT induced reproduction by inhibiting the secretion of the gonad-inhibiting hormone (GIH) from the X-organ sinus gland (eyestalk) (Khornchatri et al, 2015).…”

Section: Neurotransmittersmentioning

confidence: 99%

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Sexual dimorphism of mud crab, genusScyllabetween sexes based on morphological and physiological characteristics

et al. 2021

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“…Urea is also considered an excellent candidate for safe storage of ammonia and hydrogen. It is a non‐toxic chemical that can be found in natural systems as well as mammal waste (urine) . The oxidation of urea contained in urine by means of self‐powered electrodes may be suitable for feeding various detection devices that are able to quantify the concentration of biomolecules that may be present in urine itself, as in the case of neurotransmitters .…”

Section: Introductionmentioning

confidence: 99%

NiAl Layered Double Hydroxides and PdNiO as Multifunctional Anodes for Prospective Self‐Powered Lab‐on‐a‐Chip Dopamine Sensors

et al. 2018

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The purpose of this work is the evaluation of two bimetallic materials, PdNiO and NiAl-layered double hydroxides (NiAl-LDHs) for their prospective use in Lab-on-a-Chip devices, in which urea contained in human urine is used as fuel to provide the energy required by the device and dopamine in the sample is detected. A urea microfluidic fuel cell, using human urine as fuel and NiAl-LDHs and PdNiO as anodes, was constructed and evaluated, resulting in a cell potential of 1 V with 50.19 mW/cm 2 power density for NiAl-LDHs and 0.9 V and 32.94 mW/cm 2 for PdNiO. The multifunctionality of these anodes was extended for detecting dopamine with detection limits of 7.38 10 À8 M and 5.46 10 À6 M for NiAl-LDHs and PdNiO, respectively. The higher content of hydroxides on NiAl-LDHs than PdNiO material allowed a better activity for urea oxidation and dopamine detection. These results show the prospects for self-powered biomedical Lab-on-a-Chip device development.

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Distribution of serotonin and dopamine in the central nervous system of the female mud crab, Scylla olivacea (Herbst)

Cited by 20 publications

References 42 publications

Visualization and Identification of Neurotransmitters in Crustacean Brain via Multifaceted Mass Spectrometric Approaches

Visualization and Identification of Neurotransmitters in Crustacean Brain via Multifaceted Mass Spectrometric Approaches

Sexual dimorphism of mud crab, genusScyllabetween sexes based on morphological and physiological characteristics

NiAl Layered Double Hydroxides and PdNiO as Multifunctional Anodes for Prospective Self‐Powered Lab‐on‐a‐Chip Dopamine Sensors

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