Distribution of S-100b protein in normal salivary glands and salivary gland tumors

Abstract

Immunohistochemical studies were performed for the presence of S-100b protein in non-neoplastic and neoplastic salivary gland tissues by the peroxidase anti-peroxidase (PAP) method. Some cases of pleomorphic adenoma were investigated by immuno-electron microscopy. S-100b protein could not be detected in epithelial cells of intercalated ducts, acini, striated ducts and excretory ducts of non-neoplastic salivary gland. However, myoepithelial cells surrounding the acini and intercalated ducts were specifically stained by S-100b protein. In pleomorphic adenomas, S-100b protein-positive cells could be mostly observed in the myxoid and chondroid areas, and the basal layer cells of the double-layered ductal cells were also positive. In clear cell adenoma, the clear cells were also S-100b protein positive. In adenoid cystic carcinomas, S-100b protein-positive cells could be found in trabecular areas, but not in tumor cells showing cribriform-pattern. In other tumors (Warthin's tumor, oxyphilic adenoma, basal cell adenoma, mucoepidermoid tumor and acinar cell carcinoma), S-100b protein positive cells were seldom observed. Immuno-electron microscopically, S-100b protein was diffusely distributed in the cytoplasm of myoepithelial cells as well as of tumor cells of pleomorphic adenoma, being distributed especially on the membrane of endoplasmic reticulum and the outer nuclear membrane.

Sialadenoma papilliferum: a case report and immunohistochemical study review

“…Initially, S-100 protein was the most popular marker for MEC, but subsequent studies found it unreliable, because it can also be expressed in ductal cells 18 . Accordingly, SMA was adopted as a standard marker for normal and neoplastic MEC, but later study showed that neoplastic MEC may not express SMA 19 .…”

Immunohistochemical Study of the Distribution of S-100 Protein in Pleomorphic Adenomas of Minor Salivary Glands.

“…HARA et al [13] and TAKAHASHI et al [20] found no S-100 protein in epithelial cells of intercalated ducts, acini, striated ducts and excretory ducts. However , myoepithe- lial cells surrounding acini and intercalated ducts were specifically positive for S-100 protein [19][20][21].…”

“…Rare or no staining has been found in oxyphilic adenoma, basal cell adenoma, Warthin's tumor, mucoepidermoid carcinomas and acinic cell carcinomas [13,18]. However interdigitating cells identified as Langerhan's cells, which stained positive for S-100 protein, have been found in the lymphoid component of Warthin's tumor [13,20].…”

“…However interdigitating cells identified as Langerhan's cells, which stained positive for S-100 protein, have been found in the lymphoid component of Warthin's tumor [13,20].…”

Primary Epithelial Tumors of Salivary Glands.Histomorphological and Immunohistochemical Implications.

“…Most of the acinic cell carcinoma show immunoreactive cytokeratins suggesting the tumor cells as originating from the ductal cells [176]. Immunoreactivity for 5-100 protein has been found in intercalated duct like cells while some studies have suggested no immunoreactivity for the 5-100 protein [82,98,126,199]. Immunoreactive vasoactive intestinal peptide, associated with neuroendocrine or neuroepithelial differentiation has also been reported in acinic cell carcinomas [87,176].…”